Megastigmane glycosides from Heterosmilax yunnanensis and their neuroprotective activity.

Ten previously undescribed megastigmane glycosides (1-5, 8-12) and four known compounds (6-7, 13-14) were isolated from the roots of Heterosmilax yunnanensis Gagnep. Their planar structures were determined by NMR, HRESIMS, UV and IR spectroscopic data. The absolute configurations were established through Rh(OCOCF)-induced ECD, quantum computational chemistry, experimental ECD and C chemical shift.

Heteryunine A, an amidated tryptophan-catechin-spiroketal hybrid with antifibrotic activity from Heterosmilax yunnanensis.

An unprecedented spiro-C-glycoside adduct, heteryunine A (1), along with two uncommon alkaloids featuring a 2,3-diketopiperazine skeleton, heterpyrazines A (2) and B (3), were discovered in the roots of Heterosmilax yunnanensis. The detailed spectroscopic analysis helped to clarify the planar structures of these compounds. Compound 1, containing 7 chiral centers, features a catechin fused with a spiroketal and connects with a tryptophan derivative by a CC bond.

Fourteen new 2-benzylbenzofuran glycosides with cardioprotective activity from Heterosmilax yunnanensis.

Fourteen new 2-benzylbenzofuran O-glycosides (1-13, 15) and one new key precursor, diarylacetone (14) were isolated from the roots of Heterosmilax yunnanensis Gagnep, which all have characteristic 2,3,4-O-trisubstituted benzyl. Their structures were elucidated by 1D and 2D NMR, HRESIMS, UV and IR. The isolated compounds were assessed for their cardioprotective activities and compounds 1, 3 and 6 could significantly improve cardiomyocytes viability.

Association of Interleukin-6 Genetic Polymorphisms (rs1800795, -174C > G and rs1800796, -572G > C) With Risk of Essential Hypertension in the Chinese Population.

Background Interleukin-6 () plays a critical role in essential hypertension (EH) and cardiovascular disease. Evidence suggests two hotspot single nucleotide polymorphisms (SNPs) of the  gene (rs1800795, -174C > G and rs1800796, -572G > C) might be associated with the susceptibility of EH. However, no consensus has yet been established.

[Anti-tumor and analgesic activity evaluation and mechanism of Compound Kushen Injection].

This study aims to evaluate the anti-tumor and analgesic activities of Compound Kushen Injection(CKI) based on zebrafish model in vivo and investigate the anti-tumor mechanism. To be specific, zebrafish tumor xenotransplantation model was established by microinjection of murine LPC H12 cells into yolk sac. Then the high-dose CKI(H-CKI), medium-dose CKI(M-CKI), low-dose CKI(L-CKI) groups, and the model group were set.

[Arthroscopic debridement combined with platelet-rich plasma injection for the treatment of Kellgren-Lawrence gradeⅠ-Ⅲ knee osteoarthritis].

Objective: To explore clinical effect of arthroscopic debridement combined with platelet-rich plasma (PRP) injection for Kellgren-Lawrence(K-L) gradeⅠ-Ⅲ knee osteoarthritis (KOA) .

Methods: Totally 117 patients with KOA who underwent arthroscopic debridement combined with injection from November 2015 to January 2019 were retrospectively analyzed. According to different injection drugs, the patients were divided into sodium hyaluronate group(group A) and PRP group(group B).

Switching dynamics analysis of forest-pest model describing effects of external periodic disturbance.

A periodically forced Filippov forest-pest model incorporating threshold policy control and integrated pest management is proposed. It is very natural and reasonable to introduce Filippov non-smooth system into the ecosystem since there were many disadvantageous factors in pest control at fixed time and the threshold control according to state variable showed rewarding characteristics. The main aim of this paper is to quest the association between pests dynamics and system parameters especially the economical threshold , the amplitude and frequency of periodic forcing term.

Complexity dynamics and simulations in a discrete switching ecosystem induced by an intermittent threshold control strategy.

Pest control is a worldwide challenge. An approach that has been developed to meet this challenge is the integrated pest management (IPM) strategy, which aims to offer environmentally sensitive solutions to pest problems, and takes into account the complex dynamics involved in the design of controlling pests. In this paper, we propose a discrete switching host-parasitoid model with a threshold control strategy, meanwhile, provide some qualitative analyses of the complexity of dynamic behaviors of the model that includes single and multi-parameter bifurcations and chaos.

[Safety evaluation of heavy metals contaminated Astragalus membranaceus using health risk assessment model].

To evaluate the safety of heavy metals contaminated Astragalus membranaceus,an appropriate protocol was established to study the heavy metals pollution level by health risk assessment. This study provided a detailed procedure to assess the medicinal herbs in quality control and safety evaluation,and expected to create awareness among the public on the safety of consuming of A. membranaceus or any other kinds of medicinal herbs.

Anti-tumor activities of active ingredients in Compound Kushen Injection.

Kushen (Radix Sophorae Flavescentis) has a long history of use for the treatment of tumors, inflammation and other diseases in traditional Chinese medicine. Compound Kushen Injection (CKI) is a mixture of natural compounds extracted from Kushen and Baituling (Rhizoma Smilacis Glabrae). The main principles of CKI are matrine (MT) and oxymatrine (OMT) that exhibit a variety of pharmacological activities, including anti-inflammatory, anti-allergic, anti-viral, anti-fibrotic and cardiovascular protective effects.

Rapid clinical induction of bupropion hydroxylation by metamizole in healthy Chinese men.

Aims: This study aimed to investigate the effect of metamizole on bupropion hydroxylation related to different CYP2B6 genotype groups in healthy volunteers.

Methods: Sixteen healthy male volunteers (6 CYP2B6*1/*1, 6 CYP2B6*1/*6 and 4 CYP2B6*6/*6) received orally administered bupropion alone and during daily treatment with metamizole 1500 mg day(-1) (500 mg tablet taken three times daily) for 4 days. Serial blood samples were obtained up to 48 h after each bupropion dose.

[Studies on chemical constituents of Heterosmilax yunnanensis].

Objective: To study the chemical constituents of Heterosmilax yunianensis.

Methods: The compounds were isolated and repeatedly purified with chromatograph and the structures were elucidated by physico-chemical properties and spectral analysis.

Results: Eight compounds were obtained and elucidated as beta-sitosterol (I), glycerol monopalmitate (II), daucosterol (IIl), hexacosanoic acid (IV), 5-hydroxymethyl furaldehyde (V), hergapen (VI), ursolic acid (VII), liquiritigenin (VIII).

[Determination of papaverine in Qiangli Pipalu by HPLC].

Objective: To establish a method for the determination of the papaverine content in Qiangli Pibalu by HPLC.

Method: A C18 column with a solvent system of acetonitrile-0.02 mol x L(-1) sodium dihydrogen phosphate (0.