Pre-diabetes virtual health management community (VHMC) intervention and group interaction management model in China: a randomised clinical trial protocol.

Introduction: Individuals with pre-diabetes are at high risk for developing type 2 diabetes mellitus (T2DM), which makes them prone to serious complications such as stroke, kidney failure, blindness and lower-limb amputation. Pre-diabetes can be reversed, and lifestyle modification is considered the best intervention method for diabetes prevention. However, it is difficult for individuals with pre-diabetes to maintain a long-term modified healthy lifestyle owing to psychological burnout in daily management over time due to poor adherence.

A multi-parametric MRI study on changes in the structure, function, and connectivity of thalamic subregions and their relationship with cognitive impairment in patients with subcortical ischemic vascular disease.

Introduction: Prior researches have reported abnormal changes of thalamus in patients with subcortical ischemic vascular disease (SIVD), which was usually analyzed as a whole. However, it was currently unclear whether the structure, function and connectivity of thalamic subregions were differentially affected by this disease and affected different cognitive functions.

Methods: This study recruited 30 SIVD patients with cognitive impairment (SIVD-CI), 30 SIVD patients with cognitive unimpaired (SIVD-CU) and 32 normal controls.

LIX1L aggravates MASH-HCC progression by reprogramming of hepatic metabolism and microenvironment via CD36.

Limb expression 1-like protein (LIX1L) is an essential player in liver disorders, but its function in metabolic dysfunction-associated steatohepatitis (MASH) and associated hepatocellular carcinoma (HCC) progression remains obscure. Here, we identify LIX1L as a key integrative regulator linking lipid metabolism and inflammation, adipose tissue and hepatic microenvironment, which promotes MASH progression. LIX1L significantly upregulates in MASH patients, mouse models, and palmitic acid-stimulated hepatocytes.

Global and Regional Burden of Bloodstream Infections Caused by Carbapenem-Resistant Gram-Negative Bacteria in 2019: A Systematic Analysis from the MICROBE Database.

Objectives: To quantify the global and regional burden of bloodstream infections associated with and attributable to carbapenem-resistant Gram-negative bacteria.

Methods: We extracted data from the MICROBE database, which includes the estimated burden of 23 pathogens and 88 pathogen-drug combinations across 12 major infectious syndromes globally in 2019. The number and rate of deaths, as well as disability-adjusted life-years linked to bloodstream infections, were systematically analyzed.

Structural features, physiological functions and digestive properties of phosphorylated corn starch: A comparative study of four phosphorylating agents and two preparation methods.

Phosphorylation is an important modification to modulate functional and digestive properties of starches. We systematically investigated starch phosphorylation process parameters by using two different preparation methods (slurry and semi-dry conditions) and four commonly used phosphorylating agents, namely sodium tripolyphosphate (STPP), sodium trimetaphosphate (STMP), STMP/STPP (99: 1), and sodium phytate (SP). The effects of phosphorylation on physicochemical characteristics, techno-functionalities, digestive properties and structural features of corn starch were analyzed.

Regulation of lactate accumulation in bovine mammary epithelial cells by LPS-induced HIF-1α/MCT1 pathway.

Lactate has been increasingly recognized for its role in diseases progression, necessitating a deeper understanding of its metabolic processes and regulatory mechanisms. This study aimed to evaluate the impact of lipopolysaccharide (LPS) on lactate accumulation in bovine mammary epithelial cells (BMECs) and to elucidate the underlying regulatory mechanisms. Further optimization of LPS treatment points was achieved by assessing the content of key glycolytic enzymes-hexokinases (HK), pyruvate kinase (PK) and pyruvate dehydrogenase (PDH)-as well as the expression levels of HK2, pyruvate dehydrogenase kinase4 (PDK4) and lactate dehydrogenase (LDHA).

Structural Basis of the Bifunctionality of Marinobacter salinexigens ZYF650 Glucosylglycerol Phosphorylase in Glucosylglycerol Catabolism.

2-O-α-Glucosylglycerol (GG) is a natural heteroside synthesized by many cyanobacteria and a few heterotrophic bacteria under salt stress conditions. Bacteria produce GG in response to stimuli and degrade it once the stimulus diminishes. Heterotrophic bacteria utilize GG phosphorylase (GGP), a member of the GH13_18 family, via a two-step process consisting of phosphorolysis and hydrolysis for GG catabolism.

Consistent presence of cutaneous artery angiosome of the caudal gluteal artery suggests the usefulness of a caudal gluteal axial pattern flap in dogs.

Objective: The purpose of this study was to define the angiosome of a cutaneous artery arising from the caudal gluteal artery and identify landmarks for its use as an axial pattern flap.

Methods: This was an experimental anatomic study done between July 2019 and July 2021 with a retrospective review of CT scans. Twenty postcontrast CT scans in client-owned dogs, unrelated to this study, were evaluated for identification of a potential angiosome over the hip region.

Green and sustainable production of isofraxidin from Acanthopanax senticosus with cellulose-based immobilized probiotics.

This study utilizes deep eutectic solvent (DES)-assisted enhancement of cellulose-based immobilized probiotics for efficient biotransformation of isofraxidin from Acanthopanax senticosus. Among seven probiotic strains tested, Lactiplantibacillus plantarum CICC 20767 exhibited the best catalytic activity. We explored the effects of 12 different DESs with varying hydrogen bond donors on the conversion capacity of the immobilized probiotics, with the highest efficiency observed using ChCl/EG (4.

Inhibiting HnRNP L-mediated alternative splicing of EIF4G1 counteracts immune checkpoint blockade resistance in Castration-resistant prostate Cancer.

Immunotherapy with checkpoint inhibitors produced significant clinical responses in a subset of cancer patients who were resistant to prior therapies. However, Castration-resistant prostate cancer (CRPC) is seriously lack of T cell infiltration, which greatly limits the clinical application of immunotherapy, but the mechanism is unclear. In the present study, in silico analyses and experimental data show that HnRNP L was significantly negatively correlated with CD4+ and CD8+ T cells infiltration in patients; besides, we found deficiency of HnRNP L recruites CD4+ and CD8+ T cells infiltration and impairs tumorigenesis.

Targeting IL-20 alleviates inflammatory mechanical allodynia and reduces epidural fibrosis in post-laminectomy syndrome rat model.

Background: Post-laminectomy syndrome (PLS) manifests as recurrent chronic back pain, with or without radiating leg pain, affecting 10-40% of patients following laminectomy. While surgical interventions can alleviate recurrent disc herniation or joint instability, medical management of PLS remains challenging due to unsatisfactory outcomes. Epidural fibrosis is a frequent cause of PLS, leading to nerve root tethering and dural sac compression.

Reduced graphene oxide prepared by cold plasma green treatment in liquid phase for fluorescence biosensing of tropomyosin in shrimp.

Graphene oxide (GO), renowned for its two-dimensional structure and exceptional fluorescence quenching capabilities, is a preferred choice for the construction of fluorescence biosensors. As the sensitivity demands for these sensors escalate, enhancing the fluorescence quenching performance of GO and reducing background fluorescence become paramount to optimize the sensor sensitivity. In this study, the use of cold plasma (CP) treatment with glucose solution as a reducing agent to refine GO into reduced graphene oxide (r-GO) with optimal fluorescence quenching abilities was explored.

Bacterial surface informatics reliant on multi-mechanism simultaneous detection for Salmonella typhimurium and Staphylococcus aureus.

Fully excavating and utilizing the rich information presented on bacterial surfaces can open innovative solutions for the multi-mechanism detection of food-borne pathogens. In this work, a colorimetric-fluorescence dual-signal lateral flow immunoassay was used to establish a simultaneous detection strategy integrating five physical, chemical, and biometric combining mechanisms for Salmonella typhimurium (S. typhimurium) and Staphylococcus aureus (S.

A bibliometric analysis of research trends and hotspots of pilocytic astrocytoma from 2004 to 2023.

Pilocytic astrocytoma (PA) is a WHO grade I neoplasm with a favorable prognosis. It is the most common pediatric benign tumor. Recently, PA has attracted more and more attention and discussion from scholars.

Anticancer effects of PEP06 (TB01) in combination with Trifluridine/Tipiracil (TAS-102) in a xenograft model of human colorectal cancer.

Background: Colorectal cancer (CRC) is the third most common cancer globally, with advanced stages presenting significant treatment challenges. Recently years, drug combination therapy has become a promising strategy for cancer treatment.

Objective: To evaluate the therapeutic efficacy of the combination of the anti-angiogenic drug PEP06 (TB01) and the cytotoxic drug Trifluridine/Tipiracil (TAS-102) in human CRC HCT-116 xenograft mouse model.

Health Communication and Adherence to Noninvasive Ventilation in Chronic Hypercapnic Respiratory Failure: A Randomized Clinical Trial.

Importance: Domiciliary noninvasive ventilation (NIV) is a standard treatment for improving health outcomes among patients with chronic hypercapnic respiratory failure (CHRF). However, poor adherence substantially limits its therapeutic effectiveness.

Objective: To determine the effect of an information-motivation-behavioral (IMB) skills-based intervention (IMB-NIV program) on NIV adherence, patient-reported health outcomes, and health service use among patients with CHRF.

Elevated Astrocytic NFAT5 of the hippocampus increases epilepsy susceptibility in hypoxic-ischemic mice.

Objective: Hypoxic-ischemic brain damage (HIBD) is a leading cause of neonatal mortality, resulting in brain injury and persistent seizures that can last into the late neonatal period and beyond. Effective treatments and interventions for infants affected by hypoxia-ischemia remain lacking. Clinical investigations have indicated an elevation of nuclear factor of activated T cells 5 (NFAT5) in whole blood from umbilical cords of severely affected HIBD infants with epilepsy.

Investigation of a hemophilia family with one female hemophilia A patient and 12 male hemophilia A patients.

Hemophilia A (HA) is an X-chromosome-linked recessive genetic disorder. Female carriers may have bleeding symptoms, but rarely have moderate or severe disease. We identified a female patient with moderate HA by pedigree tracking and genetic testing in a HA family involving consanguineous marriage.

A Multisite Randomized Feasibility Trial of a Remotely Delivered Pelvic Yoga Program for Women with Chronic Pelvic Pain Syndrome.

Yoga has been recommended as a complementary management strategy for women with chronic pelvic pain (CPP), but many women lack access to specialized yoga instruction for this indication, and few data are available to evaluate changes in CPP with yoga. This feasibility trial evaluated the acceptability and tolerability of a remotely delivered yoga program for CPP in women and examined data quality and interpretability for measures of pelvic pain intensity and impact with yoga instruction. Ambulatory women with CPP were recruited from northern California in 2020-2022 and randomly assigned to a 2-month program involving twice weekly group classes delivered by videoconference supplemented by individual practice of pelvic yoga techniques versus a control program involving equivalent-time instruction and practice of nonspecific skeletal muscle stretching-strengthening exercises.

Synergistic Ultrasound-Activable Artificial Enzyme and Precision Gene Therapy to Suppress Redox Homeostasis and Malignant Phenotypes for Controllably Combating Hepatocellular Carcinoma.

Hepatocellular carcinoma (HCC) remains one of the most lethal malignant tumors. Multimodal therapeutics with synergistic effects for treating HCC have attracted increasing attention, for instance, designing biocompatible porphyrin-based nanomedicines for enzyme-mimetic and ultrasound (US)-activable reactive oxygen species (ROS) generation. Despite the promise, the landscape of such advancements remains sparse.

The inclusion membrane protein Cpn0308 interacts with host protein ACBD3.

is an obligate intracellular bacterium of eukaryotic cells characterized by a unique biphasic life cycle; its biosynthesis and replication must occur within a cytoplasmic vacuole or inclusion. Certain inclusion membrane proteins have been demonstrated to mediate the interactions between intra-inclusion chlamydial organisms and the host cell. It has been demonstrated previously that the -encoded Cpn0308 localizes to the inclusion membrane; however, its function remains unknown.

Scalable Top-Down Approach for Recycling Highly Degraded Spent LiFePO via Lattice Fragmentation-Regeneration.

Designing efficient, scalable, and eco-friendly recycling technologies is crucial for addressing the widespread decommissioning of spent lithium-ion batteries. Here, an innovative top-down regeneration method is introduced to rejuvenate highly degraded LiFePO. Initially, the crystal structure of spent LiFePO is destroyed via the oxidation process, followed by the reconstruction of the LiFePO lattice through the reduction process.