Ag/Pyridine Co-Mediated Oxidative Arylthiocyanation of Activated Alkenes.

An efficient Ag/pyridine co-mediated oxidative arylthiocyanation of activated alkenes via radical addition/cyclization cascade process was developed. This reaction could be carried out under mild conditions to provide biologically interesting 3-alkylthiocyanato-2-oxindoles in good to excellent yields. Mechanistic studies suggested a unique NCS• radical addition path and clarified the dual roles of catalytic pyridine as base and crucial ligand to accelerate the oxidation of Ag(I) to Ag(II), which is likely oxidant responsible for the formation of NCS• radical.

A novel FBN2 mutation in a Chinese family with congenital contractural arachnodactyly.

Congenital contractural arachnodactyly (CCA, OMIM: 121050) is an autosomal dominant condition that shares skeletal features with Marfan syndrome (MFS, OMIM: 154700), including contractures, arachnodactyly, dolichostenomelia, scoliosis, crumpled ears and pectus deformities but excluding the ocular and cardiovascular complications that characterize MFS. These two similar syndromes result from mutations in two genes belonging to the fibrillin family, FBN1 and FBN2, respectively. We successfully identified a novel FBN2 mutation (C1406R) in a Chinese family with CCA for over five generations.

[Expression of HCGbeta-CTP polymeride by the methylotropic yeast, pichia pastoris and structure prediction of the expressed products].

To investigate the expression of CTP encoding gene in the methylotropic yeast, pichia pastoris and the possibility of CTP acting as an antifertility vaccine or anti-cancer vaccine, we strung two, three or four CTP cDNA to construct CTP polymeric cDNA in order to enhance the immunogenicity of the CTP. Then, the recombinant genes were subcloned into a pichia pastoris expression vector pPIC9K to construct pPIC9K-(hCGbeta-CTP37)n(n = 2,3,4). After identified by restriction endonuclease digestion and DNA sequencing, the recombinant vectors were linearized and transferred into GS115 by electroporation.

[Biotransformation of dehydroepiandrosterone by hairy root cultures of Anisodus tanguticus].

Aim: To modify the structure of dehydroepiandrosterone (DHEA).

Methods: Using hairy root cultures of Anisodus tanguticus to perform biotransformation of DHEA, using chromatographic and spectral techniques to isolate and identify the products.

Results: (1) The MS medium without plant hormone was suitable for the growth of the hairy root.