Publications by authors named "Wen-Hang Yang"
Article Synopsis
- * A new method called Coulter counter-based antimicrobial susceptibility testing (CAST) was developed, allowing for fast analysis of bacterial growth after just 2 hours of exposure to antibiotics.
- * Testing showed that CAST performed effectively, with results matching traditional methods 90.18% of the time, making it a promising tool for clinical use.
Article Synopsis
- Type II complex is linked to both invasive and non-invasive infections in humans, with 11 strains identified from samples collected in China using advanced laboratory techniques.
- Whole genome sequencing was performed to analyze genotypes, revealing high minimum inhibitory concentrations (MICs) to amphotericin B and one isolate showing extreme resistance to 5-flucytosine.
- These findings suggest that the strains primarily pose a risk to patients with weakened immune systems, and the resistance to key antifungal treatments could complicate clinical management.
Article Synopsis
- A rare fungal variant has been linked to human infections, necessitating accurate identification through DNA sequencing techniques, specifically targeting the rDNA internal transcribed spacer (ITS) region.
- This study involved collecting two strains from a Chinese hospital to compare the identification capabilities of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and VITEK 2 YST ID biochemical methods against conventional ITS sequencing.
- Results showed that both strains were misidentified at the species level by the MS methods and exhibited significant drug resistance, highlighting the emergence of drug-resistant fungal species in China that pose a risk for invasive diseases.
The use of morphology to diagnose invasive mould infections in China still faces substantial challenges, which often leads to delayed diagnosis or misdiagnosis. We developed a model called XMVision Fungus AI to identify mould infections by training, testing, and evaluating a ResNet-50 model. Our research achieved the rapid identification of nine common clinical moulds: complex, complex, complex, complex, , , , spp.
View Article and Find Full Text PDFThis study aimed to assess the diagnostic values of peptidoglycan (PGN), lipopolysaccharide (LPS) and (1,3)-Beta-D-Glucan (BDG) in patients with suspected bloodstream infection. We collected 493 heparin anticoagulant samples from patients undergoing blood culture in Peking Union Medical College Hospital from November 2020 to March 2021. The PGN, LPS, and BDG in the plasma were detected using an automatic enzyme labeling analyzer, GLP-F300.
View Article and Find Full Text PDFAntifungal Susceptibility Profiles and Resistance Mechanisms of Clinical Isolates With High MIC Values.
Front Cell Infect Microbiol
November 2021
() is an ascomycete yeast species widely used in environmental and industrial research and capable of causing infections in humans and animals. At present, there are only a few studies on , and further research is required for its more in-depth characterization and analysis. Eleven strains of collected from China Hospital Invasive Fungal Surveillance Net (CHIF-NET) and the CHIF-NET North China Program were identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry and internal transcribed spacer sequencing.
View Article and Find Full Text PDFFilamentous fungi identification by Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been challenging due to the lack of simple and rapid protein extraction methods and insufficient species coverage in the database. In this study, we created two rapid protein extraction methods for filamentous fungi: a one-step zirconia-silica beads method (ZSB) and a focused-ultrasonication method (FUS). The identification accuracy of two methods were evaluated with the VITEK MS, as well as number of spectra peaks and signal-to-noise ratio (S/N) with M-Discover 100 MALDI-TOF MS compared to the routine method.
View Article and Find Full Text PDFGLUT3 as an Intersection of Glycerophospholipid Metabolism and the Innate Immune Response to .
Front Cell Infect Microbiol
July 2021
Immune cells can optimize the management of metabolic resources to balance their energy requirements in order to regulate immune responses. The interconnection between immunometabolism and fungal infections is becoming increasingly apparent. Using proteome and metabolome assays, we found that stimulation of primary human monocytes by was accompanied by upregulation of glucose transporter 3 (GLUT3) and activation of the glycerophospholipid metabolism pathway.
View Article and Find Full Text PDFArticle Synopsis
- A study collected 111 fungal isolates, including 103 Penicillium and 8 Talaromyces, from clinical sources in a Beijing medical center between 2016-2017, confirming identification through genetic sequencing.
- Findings revealed that Penicillium oxalicum was the most common species among Penicillium isolates, while Talaromyces funiculosus was dominant among Talaromyces isolates; both groups exhibited significant growth at 37°C.
- Antifungal tests showed that azoles and amphotericin B were effective against T. marneffei, whereas echinocandins had the lowest minimum effective concentrations (MEC) for most species, highlighting the need for further understanding of
Article Synopsis
- A study analyzed the distribution and drug resistance of Aspergillus species in patients with otomycosis in northern China between 2017 and 2018.
- The most common species found was A. welwitschiae, followed by A. tubingensis and A. niger, with some other species also identified.
- Most isolates were susceptible to amphotericin B and posaconazole, but a small percentage showed resistance to azoles, highlighting the need for careful treatment strategies.
Background: Omadacycline (ZL-2401) is a semi-synthetic derivative of minocycline. It has a broadspectrum activity against Gram-positive and Gram-negative bacteria, and atypical pathogens. The objective of this study was to evaluate the antibacterial activity of omadacycline against recently collected bacterial isolates from Chinese patients.
View Article and Find Full Text PDFLaboratory diagnosis of COVID-19 in China: A review of challenging cases and analysis.
J Microbiol Immunol Infect
February 2021
Article Synopsis
- - The COVID-19 outbreak, starting in Wuhan, quickly escalated into a global pandemic, highlighting the urgent need for reliable diagnostic methods to control its spread and protect public health.
- - High false-negative rates (41%) in qRT-PCR tests for detecting SARS-CoV-2 and various limitations of serological testing in China have complicated accurate COVID-19 diagnosis.
- - The review explores various molecular techniques for detecting SARS-CoV-2, such as qRT-PCR and CRISPR/Cas13, while addressing challenges like false negatives and potential solutions for improving diagnostic accuracy.
Article Synopsis
- A study was conducted at Peking Union Medical Hospital from 2013 to 2018 to determine the distribution of non-tuberculous mycobacteria (NTM) among tuberculosis specimens using DNA microarray technology.
- NTM was found in 37.3% of the 1514 specimens analyzed, with a significant increase in prevalence from 15.6% in 2013 to 46.1% in 2018. Most affected patients were females aged 45-65.
- The most common NTM species identified were Mycobacterium intracellulare and Mycobacterium chelonae/Mycobacterium abscessus, highlighting the need for accurate identification for effective treatment.
Background/purpose: As the incidence of fungal infections in China increases, the demand for rapid and accurate diagnosis of mycoses is growing. Yet, information on current diagnostic capacity is scarce.
Methods: An online survey was conducted in February 2018 to collect information on mycology testing from tertiary care hospitals across China.
Article Synopsis
- This study examined the diversity of soil actinobacterial communities in both urban and rural areas of the hydro-fluctuation zone in the Three Gorges Reservoir region of Chongqing.
- It found that actinobacterial biodiversity was significantly higher in urban areas compared to rural areas, especially in surface soil, and that woodland soils had higher biodiversity than grasslands.
- Key factors influencing biodiversity differed between urban and rural zones, with water content and nitrogen being critical in urban areas, while multiple soil nutrients impacted the rural biodiversity.