An efficient method of recovering and recycling solvent for counter-current chromatography was established by which zeaxanthin was separated from Lycium barbarum L. fruits. A column with activated carbon combined with high performance counter-current chromatography formed the recovering and recycling solvent system.
View Article and Find Full Text PDFThe chiral separation by counter-current chromatography has made great progress in the past three decades. It has become increasingly popular in the field of chiral separation, and many applications have been introduced during the last years. This review mainly focuses on the current topics, applications, and trends in chiral separation by counter-current chromatography.
View Article and Find Full Text PDFElution-extrusion counter current chromatography extrudes the most solute retained in the column with the highest possible peak resolution. It can greatly improve the hydrophobic window. In recent years, elution-extrusion counter current chromatography has received extensive attention in the separation of complex samples.
View Article and Find Full Text PDFAntioxidants play an essential role in human health, as they have been found to be capable of lowering the incidence of many diseases, such as cancer and angiocardiopathy. Currently, more attention is paid to natural antioxidants because of the possible insecurity of synthetic antioxidants. Thus, the development of efficient techniques or methods to separate antioxidants from natural sources is requested urgently.
View Article and Find Full Text PDFAn efficient combination strategy based on high-speed shear dispersing emulsifier technique and high-performance countercurrent chromatography was developed for on-line extraction and isolation of carotenoids from the fruits of Lycium barbarum. In this work, the high-speed shear dispersing emulsifier technique has been employed to extract crude extracts using the upper phase of high-performance countercurrent chromatography solvent system composed of n-hexane-dichloromethane-acetonitrile (10:4:6.5, v/v) as the extraction solvent.
View Article and Find Full Text PDFIn this work, to further verify and develop the novel counter-current chromatography modified column separate mode, a melamine modified counter-current chromatography column was prepared. Meanwhile, the modified counter-current chromatography column was used to separate stevioside and rebaudioside A with the same partition coefficient in chosen solvent system to evaluate its separation efficiency. The results show that because of the presence of intermolecular forces between melamine and model compounds, better separation could be achieved on the modified column while it's almost impossible to be separated on the unmodified column.
View Article and Find Full Text PDFThe stationary phase retention is one of the most important parameters in countercurrent chromatography. In this work, a simple gradient equilibrium method was developed to further improve the stationary phase retention based on the optimized condition in the traditional equilibrium model. Meanwhile, this novel gradient equilibrium method was used to separate three flavone model compounds and compared with the conventional isocratic equilibrium method to evaluate the separation efficiency.
View Article and Find Full Text PDFTo improve the separation capability of CCC, a novel solid-liquid two-stationary phases CCC (ASP-CCC) column was prepared employing graphene oxide (GO) conjugated poly-dopamine (PD) coating (GO/PD) as auxiliary stationary phase (ASP). The results of Scanning electron microscopy (SEM), contact angle and X-ray photoelectron spectroscopy (XPS) indicated that nanostructured GO and PD were successfully grafted on the inner wall of the PTFE column. Three alkaloid compounds were selected as the target analytes to evaluate the performance of the novel column.
View Article and Find Full Text PDFObjective: To study the chemical consistuents of Tamarix chinensis.
Methods: The compounds were isolated and purified by column chromatography and their structures were elucidated through spectroscopic analysis.
Results: Nine compounds were isolated and identified as isotamarixen(1), matairesinol(2), tetepathine(3), kaempferol(4), 4'-methylkaempferol(5),4',7-dimethylkaempferol (6), hexacosyl-3-caffeate(7), ferulic acid(8) and 3-methoxyl methyl gallate(9).
Objective: To study the chemical constituents of the flower of Paulownia fortunei.
Methods: The constituents were isolated by column chromatography and their structures were elucidated through spectroscopic analysis.
Results: The compounds were identified as: apigenin (I), luteolin (II), Hesperetin (III), Naringenin-7-O-beta-D-glucoside (IV), Arbutin (V), 4-hydroxybenzyl-beta-D-glucoside (VI), abscisic acid (VII) and 1-acetoxy-3-hydroxypropan-2-yl-3-hydroxypentanoate (VIII).
Objective: To study the chemical constituents of the flower of Paulownia fortunei (Seem.) Hemsl.
Methods: The constituents were isolated by column chromatography and their structures were elucidated through spectroscopic analysis.