[Methylselenocysteine Promotes Etoposide Cytotoxicity by Enhancing Homotypic Gap Junctions Composed of Connexin 26].

Objective: To investigate the effect of methylselenocysteine (MSC) on the function of homotypic gap junction (GJ) composed of connexin (Cx) 26 and its regulation of chemotherapeutic drug cytotoxicity.

Methods: The Tet-on HeLa cells transfected with and stably expressing Cx26 were used as the tool cells. Effects of MSC on cell growth, GJ function, and Cx26 protein expression were examined by MTT method, parachute assay, and Western blot analysis, respectively.

Effect of Penetration Electroacupuncture Combined with Intermediate Frequency Electrotherapy, Facial Acupoint Massage, and Cervical Reduction on Facial Nerve Function and Curative Effect of Senile Refractory Facial Paralysis.

Objective: This study aimed to explore the curative effects on senile refractory facial paralysis treated by penetration electroacupuncture combined with intermediate frequency electrotherapy, facial acupoint massage, and cervical reduction on facial nerve.

Methods: 106 elderly patients with intractable facial paralysis that were retrospectively analyzed were all admitted from January 2019 to June 2020. The 106 patients were evenly divided into 2 groups according to the treatment method.

Trans-scapular approach coil localization for scapular-blocked pulmonary nodules: a retrospective study.

Background: Preoperative computed tomography (CT)-guided coil localization (CL) is commonly used to facilitate video-assisted thoracoscopic surgery (VATS)-guided diagnostic wedge resection (WR) of pulmonary nodules (PNs). When a scapular-blocked PN (SBPN) is localized, the trans-scapular CL (TSCL) is commonly performed. In this study, we investigated the safety, feasibility, and clinical efficacy of preoperative CT-guided TSCL for SBPNs.

The complete mitochondrial genome of the freshwater crab (Decapoda: Brachyura: Potamoidea).

The complete mitochondrial genome of was obtained for the first time. The complete mitochondrial genome of is 17,130 bp in length, including 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region. In addition, the mitogenome has 18 noncoding regions ranging from 1 to 1553 bp in length.

The complete mitochondrial genome of the freshwater crab (Decapoda: Brachyura: Potamoidea).

We report the complete mitochondrial genome of for the first time, which is found to be 16,547 base pairs in length, and contains 13 protein-coding genes (PCGs), two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA), and one non-coding AT-rich region known as the D-loop. In addition, the mitogenome has 17 intergenic regions ranging from 1 to 1512 bp in length. The mitochondrial genome of is the first mitochondrial genome under the genus , providing DNA data for species identification, enriching the species diversity of Brachyura, and providing a basis for further studies on population genetics and phylogenetics.

An Advanced Hand-Held Microfiber-Based Sensor for Ultrasensitive Lead Ion Detection.

This study demonstrated a l-glutathione-modified nonadiabatic microfiber sensor to detect a trace level of heavy metal ions in aqueous solution. The sensor showed an exclusively selective response to Pb among other metal ions and a measured detection limit of 5 μg/L, lower than the maximum allowable limit of Pb in drinking water by the World Health Organization. Moreover, a novel compact all-fiber-based interrogation scheme was proposed to promote the development of a portable hand-held system for on-site measurement.

Delayed introduction of immunosuppressive regimens in critically ill patients after liver transplantation.

Background: The manipulation of immunosuppression therapy remains challenging in patients who develop infectious diseases or multiple organ dysfunction after liver transplantation. We evaluated the outcomes of delayed introduction of immunosuppression in the patients after liver transplantation under immune monitoring with ImmuKnow assay.

Methods: From March 2009 to February 2014, 225 consecutive liver recipients in our institute were included.

Traditional surgical planning of liver surgery is modified by 3D interactive quantitative surgical planning approach: a single-center experience with 305 patients.

Background: Decision making and surgical planning are to achieve the precise balance of maximal removal of target lesion, maximal sparing of functional liver remnant volume, and minimal surgical invasiveness and therefore, crucial in liver surgery. The aim of this prospective study was to validate the accuracy and predictability of 3D interactive quantitative surgical planning approach (IQSP), and to evaluate the impact of IQSP on traditional surgical plans based on 2D images.

Methods: A total of 305 consecutive patients undergoing hepatectomy were included in this study.

High expression of miR-15b predicts poor prognosis for hepatocellular carcinoma after curative hepatectomy.

The present study aimed to analyze the role and mechanism of miR-15b in hepatocellular carcinoma (HCC) after curative hepatectomy. Tissue samples from 13 patients with HCC who were operated on at the Chinese PLA General Hospital from March 2014 to May 2014 were collected. A consecutive 156 untreated patients with HCC who received curative hepatectomy at the Chinese PLA General Hospital (Beijing, China) from May 2008 to March 2009 were enrolled, and their corresponding para-tumoral and normal tissue samples were acquired.

Auxiliary partial liver transplantation for acute liver failure using "high risk" grafts: Case report.

Acute liver failure (ALF) is a reversible disorder that is associated with an abrupt loss of hepatic mass, rapidly progressive encephalopathy and devastating complications. Despite its high mortality, an emergency liver transplantation nowadays forms an integral part in ALF management and has substantially improved the outcomes of ALF. Here, we report the case of a 32-year-old female patient who was admitted with grade IV hepatic encephalopathy (coma) following drug-induced ALF.

Ultrasound-guided radiofrequency ablation of the segmental Glissonian pedicle: A new technique for anatomic liver resection.

Background: Anatomic liver resection is widely accepted as the optimal surgical treatment for hepatocellular carcinoma (HCC); however, the complexity of conventional operative methods limits their use. To explore the possibility of using modern techniques to achieve a simpler approach, we have evaluated ultrasound-guided segmental radiofrequency ablation (RFA) of the Glissonian pedicle before liver resection in a porcine model and in HCC patients.

Methods: This study had 2 stages.

Robotic spleen-preserving laparoscopic distal pancreatectomy: a single-centered Chinese experience.

Background: Spleen-preserving laparoscopic distal pancreatectomy is technically challenging. New surgical robotic systems are now available and show promising outcomes but were very recently implemented in China.

Methods: Seven patients underwent laparoscopic distal pancreatectomy using the da Vinci Robotic System (RDP) for benign or borderline malignant pancreatic tumors.

Poor prognosis for hepatocellular carcinoma with transarterial chemoembolization pre-transplantation: retrospective analysis.

Aim: To investigate whether transarterial chemoembolization (TACE) before liver transplantation (LT) improves long-term survival in hepatocellular carcinoma (HCC) patients.

Methods: A retrospective study was conducted among 204 patients with HCC who received LT from January 2002 to December 2010 in PLA General Hospital. Among them, 88 patients received TACE before LT.

Graft cholangiopathy: etiology, diagnosis, and therapeutic strategies.

Background: Graft cholangiopathy has been recognized as a significant cause of morbidity, graft loss, and even mortality in patients after orthotopic liver transplantation. The aim of this review is to analyze the etiology, pathogenesis, diagnosis and therapeutic strategies of graft cholangiopathy after liver transplantation.

Data Source: A PubMed database search was performed to identify articles relevant to liver transplantation, biliary complications and cholangiopathy.

Highly sensitive refractive index sensor based on adiabatically tapered microfiber long period gratings.

We demonstrate a refractive index sensor based on a long period grating (LPG) inscribed in a special photosensitive microfiber with double-clad profile. The fiber is tapered gradually enough to ensure the adiabaticity of the fiber taper. In other words, the resulting insertion loss is sufficiently small.

[Follow-up studies for long-term postoperative complications of Warshaw operation].

Objective: To explore the long-term postoperative complications of Warshaw operation for distal pancreatectomy with preservation of spleen.

Methods: Retrospectively analyzed the clinical data of distal pancreatectomy on 84 cases between January 2006 and January 2012. Among them, 35 patients underwent Warshaw operation for distal pancreatectomy with preservation of spleen and another 23 had distal pancreatectomy with Kimura operation.

Technical points of total laparoscopic choledochal cyst excision.

Background: Choledochal cyst excision and biliary enteric reconstruction constitute the best therapy for choledochal cyst. And laparoscopy is currently used to cure this disease now.

Methods: We retrospectively analyzed the clinical data of 34 cases of total laparoscopic choledochal cyst excision between January 2007 and August 2011.

Aggressive hepatectomy for the curative treatment of bilobar involvement of type IV-A bile duct cyst.

Objective: To analyze the risk and benefit of aggressive hepatectomy for the curative treatment of bilobar bile duct cysts (BDCs) of type IV-A.

Background: Conventional surgical treatment of bilobar BDCs of type IV-A is extrahepatic cyst excision, followed by biliodigestive anastomosis. The role of hepatectomy in the treatment of bilobar BDCs remains unclear.

[Observation of laparoscopic distal pancreatectomy in hepatobiliary surgery: a report of 25 cases].

Objective: To explore the clinical application of laparoscopic distal pancreatectomy (LDP) in hepatobiliary operations.

Methods: The clinical data of LDP from 25 cases from June 2008 to December 2011 were retrospectively analyzed. Among them, 9 patients underwent LDP with excision of spleen while another 16 patients had LDP with preservation of spleen.

Interventional therapy for renal artery pseudoaneurysms.

The aim of this study was to explore the angiographic diagnosis and embolization therapy for renal artery pseudoaneurysms due to acute urinary tract hemorrhage after conservative medical management failed. Seven out of ten cases had fever symptoms after the kidney surgery. The pseudoaneurysms were treated with gelatin sponge and (or) spring coil and the majority demonstrated rapid blockage of hemorrhage.

[Intrahepatic biliary cystadenoma: experience with 10 consecutive cases at a single center].

Objective: To summarize the clinical experience with diagnosis and treatment of intrahepatic biliary cystadenoma (IBCA).

Methods: We retrospectively analyzed the data of 10 consecutive IBCA cases treated in our department in light of the characteristics of the epidemiology, radiology, lab tests, pathology and prognostic.

Results: The patients are all female with an average age of 48.

Efficacy of liver transplantation for acute hepatic failure: a single-center experience.

Background: Acute hepatic failure (AHF) is a devastating clinical syndrome with a high mortality rate. The outcome of AHF varies with etiology, but liver transplantation (LT) can significantly improve the prognosis and survival rate of such patients. This study aimed to detect the role of LT and artificial liver support systems (ALSS) for AHF patients and to analyze the etiology and outcome of patients with this disease.

Diagnosis and treatment of fungal infection after liver transplantation.

Background: Liver transplantation is the most effective treatment for end-stage liver diseases; however, infections after transplantation can seriously affect the patient's health. The aim of this research was to investigate the diagnosis and treatment of fungal infection following liver transplantation.

Methods: Clinical data for 232 liver transplant patients at risk of fungal infection were examined for the presence of fungus in the blood, fluid, sputum, urine and stools of patients and by chest or abdominal CT scans.