Programmed cell death-1 in patients with primary liver cancer and its effect on prognosis.

Purpose: To detect the expression of programmed cell death-1 (PD-1) in peripheral blood T lymphocytes of patients with primary liver cancer and its effect on their prognosis.

Methods: The medical records of 42 patients with primary liver cancer, 36 patients with chronic hepatitis B (CHB) and 38 healthy volunteers composed the liver cancer group, benign lesion group and control group, respectively. Fluorescence quantitative RT-PCR (qRT-PCR) was used for detecting the expression level of PD-1 mRNA in peripheral blood T lymphocytes of subjects in the three groups, and flow cytometry was used for detecting the positive expression of PD-l protein on the surface of T lymphocytes of subjects.

Bioinformatics analysis of microarray data to explore the key genes involved in mutation-induced cataract.

Aim: To reveal the mechanisms of 4 () mutation-induced cataract.

Methods: GSE22362, including 3 -null lens and 3 wild-type lens, was obtained from Gene Expression Omnibus database. After data preprocessing, the differentially expressed genes (DEGs) were identified using the limma package.