Development and Validation of the 9-Dye Fluorescent Autosomal and Y Chromosome Multiplex STR Amplification System.

To develop a 9-dye fluorescent autosomal and Y chromosome STR multiplex amplification system and verify its performance. Using the 9-dye fluorescent labeling technology, construct a multiplex amplification system that uses the autosomal and Y chromosome core and preferred loci recommended by GB/T 41009-2021 national standard as candidate loci, then evaluate its accuracy, sensitivity, species specificity, and other system performance index. A 9-dye fluorescent multiplex amplification system containing 70 loci, including Amel, D5S818, D21S11, D7S820, CS1FPO, D2S1338, D19S433, D8S1132, D2S441, VWA, D8S1179, D16S539, D10S1435, PentaD, D3S3045, TPOX, TH01, D22S1045, D18S51, FGA, D19S253, D10S1248, D6S477, D6S1043, D13S317, D12S391, D15S659, PentaE, D1S1656, D3S1358, DYS460, DYS389I, DYS390, DYS533, DYS392, DYS518, DYS557, DYS570, DYS458, DYS437, DYS385, GATA-H4, DYS576, DYS643, DYS508, DYS627, DYS456, DYS391, DYF404S1, DYS438, DYS448, DYF387S1, DYS596, DYS549, DYS399S1, DYS393, DYS635, DYS439, DYS19, DYS444, DYS449, DYS481, DYS527a/b, and DYS447 was successfully constructed.

Regulation of alternative splicing by CBF-mediated protein condensation in plant response to cold stress.

Cold acclimation is critical for the survival of plants in temperate regions under low temperatures, and C-REPEAT BINDING FACTORs (CBFs) are well established as key transcriptional factors that regulate this adaptive process by controlling the expression of cold-responsive genes. Here we demonstrate that CBFs are involved in modulating alternative splicing during cold acclimation through their interaction with subunits of the spliceosome complex. Under cold stress, CBF proteins accumulate and directly interact with SKI-INTERACTING PROTEIN (SKIP), a key component of the spliceosome, which positively regulates acquired freezing tolerance.

Resolving ecological drivers of temporal variations of β-diversity across intertidal microbiomes.

Resolving the ecological drivers mediating the diversity patterns of microbial communities across space and through time is a central issue in microbial ecology. Both regional species pools and local community assembly contribute to the spatial turnover of biodiversity. In this study, we extended the concept of regional species pool to temporal, and investigated the seasonal dynamics of intertidal microbiomes across four microbial domains/kingdoms (bacteria, archaea, fungi, and protists).

The role of programmed cell death in diabetes mellitus-induced erectile dysfunction: from mechanisms to targeted therapy.

Diabetes mellitus (DM) is a chronic metabolic disease that often leads to vascular endothelial injury and peripheral neuropathy. Erectile dysfunction (ED), a common condition in andrology, is frequently associated with DM. The incidence of diabetes mellitus-induced ED (DMED) is second only to the cardiovascular complications of diabetes.

Investigating the psammophilic karyorelictean ciliate families Kentrophoridae and Cryptopharyngidae (Protista, Ciliophora): molecular phylogeny, geographic distributions and a brief revision including descriptions of a new genus, a new species and a new combination.

Psammophillic ciliates are an integral part of the foodweb despite being underrepresented in terms of molecular phylogeny and modern taxonomy. To investigate the karyorelictean group, sampling was conducted in interstitial marine habitats in China for ciliates living between the sand grains, resulting in an examination of the families Cryptopharyngidae Jankowski, 1980 and Kentrophoridae Jankowski, 1980. Three species, i.

Retrograde percutaneous coronary intervention for chronic total occlusions in patients with reduced left ventricular ejection fraction: a single-center retrospective cohort study.

Background: Data on the safety and prognostic implications of retrograde percutaneous coronary intervention (PCI) in patients with low left ventricular ejection fraction (LVEF) and chronic total occlusion (CTO) are unclear. This study aimed to assess the procedural results and long-term outcomes of retrograde CTO PCI in individuals with reduced LVEF (≤ 40%).

Methods: We conducted a retrospective analysis of 836 consecutive patients who underwent elective retrograde CTO PCI at a single center between January 2011 and April 2023.

The effect of cheese intake on osteoarthritis: a Mendelian randomization study.

Introduction: Osteoarthritis (OA) is a debilitating chronic disease with a high prevalence, characterized by progressive degeneration of articular cartilage that leads to joint dysfunction, pain, and disability. Observational studies investigating the link between cheese intake and OA have yielded inconclusive results and may be susceptible to confounding.

Material And Methods: We performed a two-sample Mendelian randomization (MR) investigation to evaluate the causal association between cheese intake and OA based on genome-wide association studies (GWAS).

One-step calcined cellulose-derived NiNx nanoclusters catalyst: Unleashing non-radical peroxymonosulfate activation for accelerated p-nitrophenol degradation.

Carbon-based catalysts doped with transition metals effectively activate persulfate for the advanced oxidation of endocrine disruptors in water. However, research on nickel-doped catalysts is limited, especially regarding non-radical mechanisms, and typically focuses on synthetic organic compounds rather than natural cellulose. This study used wheat straw cellulose to synthesize NiNx nanocluster catalysts (Ni-NC) via a one-step calcination method.

Global hotspots and trends on environmental exposure and cardiovascular disease from 1999 to 2022.

Background: The increasing risk of cardiovascular disease (CVD) associated with worsening environmental exposure is a critical health concern garnering global research attention.

Aim: To systematically assess the scope and characteristics of research on the relationship between environmental exposure and CVD.

Methods: A thorough examination of publications on the relationship between environmental exposure and CVD from 1999 to 2022 was carried out by extensively screening the literature using the Web of Science Core Collection.

Pilot Study of a Modified DOPS Scale for Insulin Pump and CGM Installation Training in Chinese Medical Students During Endocrinology Rotations.

Background: Direct Observation of Procedural Skills (DOPS) is a clinical assessment tool that enables trainers to observe medical students' procedural abilities in real-time clinical settings. It assesses students' knowledge application, decision-making, and skill proficiency during clinical tasks.

Methods: This study modifies the DOPS to evaluate the operation of insulin pumps (PUMP) and continuous glucose monitoring systems (CGMS) in diabetes management.

Blocking the isoflavone chemoreceptor in to prevent disease.

Inhibiting pathogen chemotaxis is a promising strategy for reducing disease pressure. However, this strategy is currently in the proof-of-concept stage. Here, was used as a model, as its biflagellated zoospores could sense genistein, a soybean root exudate, to navigate host and initiate infection.

Self-assembled eumelanin nanoparticles enhance IFN-I activation and cilia-driven intercellular communication to defend against Tulane virus, a human norovirus surrogate.

Norovirus (NoV) infection is a leading cause of gastroenteritis and poses global health threats, with increasing incidence reported in immunocompromised individuals, which is further exacerbated by the globalization of the food industry. Eumelanin has demonstrated its potential in antiviral treatments, but its role in preventing viral infections remains underexplored. Therefore, in this study, we investigated the antiviral properties and potential mechanisms of self-assembled eumelanin nanoparticles (EmNPs) against Tulane virus (TuV), a surrogate with a similar infection mechanism to NoVs.

Association between cardiometabolic index and the incidence of stroke: a prospective nationwide cohort study in China.

Objectives: Cardiometabolic index (CMI), based on triglycerides (TG) to high-density lipoprotein cholesterol (HDL-C) ratio and waist-to-height ratio (WHtR), has been recognized as a novel and practical marker for the assessment of cardiometabolic risk. However, the relationship between CMI and the incidence of stroke remains to be elucidated. This investigation aimed to explore the association between CMI and stroke incidence.

General Acetal-Protected Aldehyde Strategy for Facile Synthesis of Covalent Organic Frameworks with Splendid Crystallinity and Uniform Morphology.

Crystallinity and morphology are critical factors that closely related to the properties and applications of covalent organic frameworks (COFs). However, the controlled synthesis of COFs with both high crystallinity and uniform morphology remains a significant challenge due to uncontrollable polymerization and complex reaction conditions. In this work, we present a general acetal-protected aldehyde protocol for the facile synthesis of imine-linked COFs, which enables the simultaneous optimization of crystallinity and morphology.

Isomeric Covalent Organic Frameworks for High-Efficiency Photocatalytic CO Reduction: Substituent Position Effect.

The exploration of covalent organic frameworks (COFs) for high-efficiency photocatalytic CO reduction is urgently demanded. Herein, COF-based catalysts are constructed for the selective photoreduction of CO to CO via delicately designed isomeric monomers with substituent at the 4,5,9,10- positions (K) or 1,3,6,8-positions (A) of pyrene knots. The distinct substituted regions significantly affect the planarity of pyrene knots, resulting in COFs with different microstructures and photocatalytic activities.

A U-shaped relationship between the atherogenic index of plasma and repeated target vessel revascularization in patients undergoing percutaneous coronary intervention: a retrospective study.

Background: Atherogenic index of plasma (AIP) has been recognized as a novel and practical marker for the assessment of cardiometabolic risk, but the relevance of AIP as a prognostic biomarker in coronary artery disease (CAD) remains debated. This study investigated the association between AIP and major adverse cardiac and cerebrovascular events (MACCEs) in CAD patients receiving percutaneous coronary intervention (PCI) with drug-eluting stents (DES).

Methods: A total of 2,250 patients undergoing PCI with DES were included in this retrospective cohort study.

Therapeutic potential of hesperidin in diabetes mellitus-induced erectile dysfunction through Nrf2-mediated ferroptosis and oxidative stress.

Background: Among erectile dysfunction (ED) caused by metabolic abnormalities, diabetes mellitus-induced ED (DMED) progresses rapidly, manifests with severe symptoms, and shows reduced responsiveness to conventional medications. Hyperglycemia in the corpus cavernosum has been linked to the induction of both ferroptosis and oxidative stress, which are mediated by nuclear factor E2 related factor 2 (Nrf2). Hesperidin (Hes), a flavonoid compound, has been revealed to activate Nrf2 in certain diabetic complications, yet the efficacy of Hes on DMED and the specific mechanism remain unclear.

The Magnetic Resonance Image-Arterial Spin Labeling Characteristic of Nonketotic Hyperglycemic Hemichorea in an Elderly Type 2 Diabetic Female Patient.

Nonketotic hyperglycemia-induced hemichorea is a rare condition of type 2 diabetes. It is characterized by hyperglycemia with the symptom traced to the basal ganglion like hemichorea or hemiballism, with the hyperintensity within basal ganglion presented in computed tomography (CT) or hyper signal in T1-weighted magnetic resonance image (MRI). It was also confirmed with a relatively better prognosis in that the symptoms of these patients could be relieved after the alleviation of hyperglycemia.

A prediction model of echocardiographic variables to screen for potentially correctable shunts in adult atrial septal defect-pulmonary hypertension patients.

Background: Atrial septal defect (ASD) is a prevalent congenital heart condition in adults that leads to pulmonary hypertension (PAH) and right heart failure if left untreated. During a routine follow-up of adult ASD-PAH patients, the suitability of shunt closure depends on the invasive right heart catheterization (RHC). Nevertheless, performing RHC at every follow-up is impractical and may be harmful.

The Relationship Between Computerized Face and Tongue Image Segmentation and Metabolic Parameters in Patients with Type 2 Diabetes Based on Machine Learning.

Objective: We aim to examine and reestablish the correlational and linear regression relationships, as well as the predictive value, between the significant facial and tongue features and the metabolic parameters in type 2 diabetes mellitus (T2DM).

Materials And Methods: From March to May 2024, we studied 269 patients with T2DM in the endocrinology department of Shanghai Pudong Hospital. The patients' facial and tongue characteristics were sampling by a tongue imaging device equipped with artificial intelligence (AI) (XiMaLife, Sinology, China) of automated and advanced machine learning algorithms.

Efficient production of singlet oxygen via dioxygen activation on Cu decorated MoS facilitates the elimination of oxytetracycline.

Molecular oxygen (O), a green oxidant, is applied in advanced oxidation processes, which represents one of the current focal points in water treatment research. However, achieving efficient and economical activation of O remains a formidable challenge because of its spin-restricted nature. Herein, zero-valent copper (Cu) modified molybdenum disulfide (MoS) materials were applied as O activators to degrade organic pollutants.

A receptor kinase senses sterol by coupling with elicitins in auxotrophic .

Sterols are vital nutrients and signals for eukaryotic organisms. Mammalian cells are known to sense and respond to sterol status changes to maintain them within strict limits, a process associated with various human diseases. However, this process is not understood in oomycete pathogens, most of which are sterol auxotrophic and must obtain sterols from host plants.