[Pseudo-targeted metabolomics for differential components of Rhei Radix et Rhizoma from three plant species].

Rhei Radix et Rhizoma is common traditional Chinese medicine with multiple original plants. The content and proportion of the active components in Rhei Radix et Rhizoma from different plant species were compared to accurately evaluate the medicine qua-lity and provide a theoretical basis for precise use of this medicine in clinical practice. In this study, fresh Rhei Radix et Rhizoma samples were collected from the four-year-old plants of Rheum palmatum, R.

Da-Chai-Hu-Tang Formula inhibits the progression and metastasis in HepG2 cells through modulation of the PI3K/AKT/STAT3-induced cell cycle arrest and apoptosis.

Ethnopharmacological Relevance: Da-Chai-Hu-Tang (DCHT), a Chinese traditional herbal compound, has been utilized for the treatment of Hepatic diseases in China for over 1800 years. The DCHT formula contains eight herbals: Bupleurum chinense DC. (chaihu), Scutellaria baicalensis Georgi (huangqin), Paeonia lactiflora Pall.

Molecular characterization, antibacterial and immunoregulatory activities of liver-expressed antimicrobial peptide 2 in black rockfish, Sebastes schlegelii.

LEAP2 (liver expression antimicrobial peptide 2), is an antimicrobial peptide widely found in vertebrates and mainly expressed in liver. LEAP2 plays a vital role in host innate immunity. In teleosts, a number of LEAP2 homologs have been reported, but their in vivo effects on host defense are still limited.

Phosphorus-doped synergy of phase change in heterogeneous catalysts of NiS-NiS for efficient electrocatalysis of Pb(II).

A fundamental understanding of the electroanalytical activity of transition metal sulfide electrocatalysts, especially the origin of the electrocatalytic reactivity on the surface sites of heterostructures with multiple crystalline phases, is essential for the design of low-cost and highly efficient nonprecious metal electrocatalysts for further scientific and technological achievements. Herein, we injected P into NiS and occupied the S sites through a doping strategy. The redistributed electronic structure induced the construction of heterostructures, which significantly improved the structure and chemical state of electrochemically inert NiS.

Modulating paired Ir-O-Ir electronic perturbations of correlated Ir single atoms to overcome catalytic selectivity.

Single-atom catalysts have been extensively utilized for electrocatalysis, in which electronic metal-support interactions are typically employed to stabilize single atoms. However, this neglects the metal-metal interactions of adjacent atoms, which are essential for the fine-tuning of selective sites. Herein, the high-loading of Ir single atoms (Ir SAs) (8.

A China-developed adenovirus vector-based COVID-19 vaccine: review of the development and application of Ad5-nCov.

Introduction: The global spread of COVID-19 has prompted the development of vaccines. A recombinant adenovirus type-5 vectored COVID-19 vaccine (Ad5-nCoV) developed by Chinese scientists has been authorized for use as a prime and booster dose in China and several other countries.

Areas Covered: We searched published articles as of 4 May 2023, on PubMed using keywords related to Adenovirus vector, vaccine, and SARS-CoV-2.

Identification, expression pattern and functional characterization of IFN-γ involved in activating JAK-STAT pathway in Sebastes schlegeli.

IFN-γ (interferon gamma) is a critical cytokine in the immune system involved both directly and indirectly in antiviral activity, stimulation of bactericidal activity, antigen presentation and activation of macrophages via the Janus kinase/signal transducer and activator of transcription (JAK-STAT) pathway. The IFN-γ function is best described in cell defense against intracellular pathogens in mammals, but IFN-γ cytokine-induced metabolic change and its role in anti-infection remain unknown in teleost fish. In this study, a novel IFN-γ (SsIFN-γ) was identified from black rockfish (Sebastes schlegeli) by rapid amplification of cDNA ends (RACE).

Transcription factor triggers the SPRY1-NF-κB pathway to maintain testicular immune homeostasis and male fertility.

Bacterial or viral infections, such as , mumps virus, herpes simplex virus, and Zika virus, destroy immune homeostasis of the testes, leading to spermatogenesis disorder and infertility. Of note, recent research shows that SARS-CoV-2 can infect male gonads and destroy Sertoli and Leydig cells, leading to male reproductive dysfunction. Due to the many side effects associated with antibiotic therapy, finding alternative treatments for inflammatory injury remains critical.

Coordination engineering strategy of iron single-atom catalysts boosts anti-Cu(II) interference detection of As(III) with a high sensitivity.

Mutual interference issues between heavy metal ions tremendously affect the detection reliability and accuracy in water quality analysis, especially the serious interference of Cu(II) on the detection of As(III) is greatly hard to overcome, which needs to be solved urgently. Herein, iron single-atom catalysts with different coordination structures of FeNC and FeNP are constructed to selectively catalyze the detection of As(III) in the coexistence of Cu(II). FeNP achieves a high sensitivity of 3.

A better overall survival (OS) for total (ipsilateral) retroperitoneal lipectomy than standard complete resection in patients with retroperitoneal liposarcoma: a comparative multi-institutional study.

Background: Complete resection (CR) serves as the standard of surgical treatment for retroperitoneal liposarcoma (RPLS). Unfortunately, even at referral centers, recurrence rates are high, and CR may not address multifocal diseases, which are a common phenomenon in RPLS. We sought to retrospectively compare the clinical outcomes of RPLS patients treated with total (ipsilateral) retroperitoneal lipectomy (TRL) and CR.

Effectiveness, Safety, and Tolerance of Scalp Cooling for Chemotherapy-Induced Alopecia.

Problem Identification: There is a lack of guideline recommendations about the use of scalp cooling for preventing chemotherapy-induced alopecia (CIA). This overview was conducted to summarize effectiveness, safety, and tolerance of scalp cooling for CIA based on systematic reviews.

Literature Search: PubMed®, Embase®, Cochrane Library, and CNKI were searched from inception to May 15, 2021.

Analysis of factors influencing cervical lymph node metastasis of papillary thyroid carcinoma at each lateral level.

Objective: To analyze the clinicopathological characteristics of patients with papillary thyroid carcinoma (PTC) and its influence on the distribution of lymph node metastasis at each lateral level of the neck to guide precise treatment of the lateral area.

Methods: The clinicopathological data of patients with PTC initially diagnosed and treated at our hospital from February 2014 to September 2021 were collected; the metastatic status of each lateral level was recorded, and correlations were analyzed.

Results: A total of 203 patients were enrolled in this study.

In-situ electronic structure redistribution tuning of single-atom Mn/g-CN catalyst to trap atomic-scale lead(II) for highly stable and accurate electroanalysis.

Constructing catalysts with simple structures, uniform effective sites, and excellent performance is crucial for understanding the reaction mechanism of target pollutants. Herein, the single-atom catalyst of Mn-intercalated graphitic carbon nitride (Mn/g-CN) was prepared. It was found that the intercalated Mn atoms acted as strong electron donors to effectively tune the electronic structure distribution of the in-situ N atoms, providing a large number of negative potential atomic-scale sites for catalytic reactions.

Antimicrobial and immunoregulatory activities of the derived peptide of a natural killer lysin from black rockfish (Sebastes schlegelii).

Natural killer lysin (NK-lysin) is a small molecule antimicrobial peptide secreted by natural killer cells and T lymphocytes. In this study, we characterized a cDNA sequence encoding an NK-lysin homologue (SsNKL1) from black rockfish, Sebastes schlegelii. The open reading frame (ORF) of SsNKL1 encodes a putative protein of 149 amino acids and shares 44%-87% overall sequence identities with other teleost NK-lysins.

Enhanced electrochemical sensing performance for trace Hg(II) by high activity of Co on CoO-NP/N-RGO surface.

Constructing a highly sensitive and selective electrochemical interface is of great significance for the effective detection of Hg in water and biological samples. Herein, CoO nanopolyhedron (NP) anchored on nitrogen-doped reduced graphene oxide (N-RGO) is utilized as the electrode material for the detection of Hg in the range of 0.1 μM-1.

Nitric oxide detection using principal component analysis spectral structure matching to the UV derivative spectrum.

Ultraviolet (UV) spectroscopy is widely applied in real-time environmental monitoring, especially in diesel vehicle nitrogen monoxide (NO) emissions. However, in field experiments, UV absorption spectrum may exist for different degrees of drifts. Spectral jitters may exist for various reasons such as optical power variation, electrical signal drift, and the refractive index jitters of the optical path for an extended period of time, which causes the detection system to be calibrated.

Zero-valent iron nanomaterial Fe@FeMnO for ultrasensitive electroanalysis of As(iii): Fe influenced surficial redox potential.

A novel zero-valent iron nanomaterial (Fe@FeMnO) was synthesized and achieved an ultrasensitive electrochemical detection of As(iii). It was found that the enhanced sensitivity is attributed to the surficial catalytic redox couple Fe(ii)/Fe(iii) induced by Fe of Fe@FeMnO. Besides, the catalytic kinetics was modelled and simulated, and the strong influence of the oxidation potential of the catalytic redox species on sensitivity was revealed.

Simultaneous measurement of phenols by three-way fluorescence spectroscopy: A comparison of N-PLS/RBL, U-PLS/RBL and PARAFAC.

Phenol, o-cresol, p-cresol, catechol and resorcinol are five phenolic compounds with extremely similar structure. Their fluorescence spectra are hard to be analyzed because of the serious spectral overlaps between any two of the five phenolic components in the mixture system. In this experiment, multi-dimensional partial least-squares (N-PLS), unfolded partial least-squares (U-PLS) with residual bilinearization (RBL) and parallel factor analysis (PARAFAC) are employed to analyze the three-way fluorescence spectra aiming to achieve quantitative results.

Nanometal Oxides with Special Surface Physicochemical Properties to Promote Electrochemical Detection of Heavy Metal Ions.

Heavy metal ions (HMIs) are one of the major environmental pollution problems currently faced. To monitor and control HMIs, rapid and reliable detection is required. Electrochemical analysis is one of the promising methods for on-site detection and monitoring due to high sensitivity, short response time, etc.

Identifying Phase-Dependent Electrochemical Stripping Performance of FeOOH Nanorod: Evidence from Kinetic Simulation and Analyte-Material Interactions.

Metal hydroxide nanomaterials are widely applied in the energy and environment fields. The electrochemical performance of such materials is strongly dependent on their crystal phases. However, as there are always multiple factors relating to the phase-dependent electrochemistry, it is still difficult to identify the determining one.

Asymmetric Formal Synthesis of (-)-Cephalotaxine via Palladium-Catalyzed Enantioselective Tsuji Allylation.

Asymmetric synthesis of the pentacyclic alkaloid (-)-cephalotaxine was accomplished via palladium-catalyzed enantioselective Tsuji allylation for construction of the aza-containing tetrasubstituted stereogenic center (95% yield, 93% ee). The allyl enol carbonate precursor was prepared from Hanaoka's ketone intermediate, which was formed by a novel formic acid promoted ring-expansion reaction.

Super-strong dislocation-structured high-carbon martensite steel.

High-carbon martensite steels (with C > 0.5 wt.%) are very hard but at the same time as brittle as glass in as-quenched or low-temperature-tempered state.

Study on Hydrogen Fluoride at High Temperature Detection Method with Temperature Correction Based on Laser Technology.

Hydrogen fluoride(HF) is one of the important character gases for fault diagnosis of gas insulation switch (GIS) in the system of substation equipment. The high-accuracy, fast- response and real-time detection method of HF is a focus in industrial and environmental fields. In this research, the HF detection experiment system was set up at first based on laser absorption spectroscopy technology combined with anti-corrosion multiple reflection cell made by monel steel.