Zafaria cholistanensis gen. nov. sp. nov., a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan.

A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated strain NCCP-1664, was isolated from Cholistan desert, Pakistan. Cells of strain NCCP-1664 were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.

[Research Advances in the Diagnostic Value of Rapid On-site Evaluation in Endobronchial Ultrasound-guided Transbronchial Needle Aspiration for Lung Cancer].

Rapid on-site evaluation(ROSE),an auxiliary sampling quality evaluation technology,can be used to evaluate the adequacy and diagnostic category of samples,judge the histological type of lung cancer,and optimize the gene type of lung cancer.Applying ROSE to endobronchial ultrasound-guided transbronchial needle aspiration of suspected lung cancer can improve the puncture success rate and diagnostic rate and reduce complications and puncture attempts.Rose performed via remote cytopathology technology or by trained respiratory specialists may become the future trends.

gen. nov., sp. nov., a member of the family , isolated from a saline and alkaline lake sediment.

A non-motile, Gram-staining negative, catalase- and oxidase-positive, crescent-rod shaped bacterium, designated strain CUG 91308, was isolated from a sediment sample of Qinghai Lake, Qinghai Province, China. Colonies on OSM agar were round, smooth, flat and pinkish-orange in colour. Strain CUG 91308 could grow at 15-37 °C, pH 6-12 and in the presence of up to 7.

Description of Salinimonas profundi sp. nov., a deep-sea bacterium harboring a transposon Tn6333.

A Gram-staining-negative bacterium, strain HHU 13199, was isolated from a marine sediment sample collected from South China Sea (119°19.896'E, 19°41.569'N) at a depth of 2918 m.

Nesterenkonia haasae sp. nov., an alkaliphilic actinobacterium isolated from a degraded pasture in Songnen Plain.

An alkaliphilic actinobacterial strain, designated Hz 6-5, was isolated from saline-alkaline soil from Songnen Plain in north-eastern China. The isolate formed light yellow-colored colonies and its cells were Gram-staining positive, non-motile, and non-spore-forming short rods. The strain was aerobic with optimal growth at 33 °C, pH 9.

Bacillus alkalicellulosilyticus sp. nov., isolated from extremely alkaline bauxite residue (red mud) site.

A Gram-stain-negative, rod-shaped, facultatively anaerobic, motile and spore-forming strain designated FJAT-44921 was isolated from red mud collected from Chiping County, Shandong Province, China. The 16S rRNA gene sequence result showed that strain FJAT-44921 shared a low sequence identity (96.6%) with the members of the genus Bacillus.

Photocatalytic reduction and anti-bacterial activity of biosynthesized silver nanoparticles against multi drug resistant Staphylococcus saprophyticus BDUMS 5 (MN310601).

In this study, silver nanoparticles (Ag NPs) was eco-friendly synthesized using purified flavonoid rich content of Morinda citrifolia (M. citrifolia) extract. The synthesized Ag NPs was exhibited at 420 nm in UV-spectrometer, and surface morphology with available chemical composition, shape and size of the Ag NPs were confirmed by X-ray diffraction (XRD) variation, scanning electron microscope (SEM) with energy dispersive X-ray spectroscopy (EDX) and transmission electron microscope (TEM).

Simultaneous quantification of six flavonoids of Rhus verniciflua Stokes using matrix solid-phase dispersion via high-performance liquid chromatography coupled with photodiode array detector.

A simple and efficient matrix solid-phase dispersion via high-performance liquid chromatography coupled with a photodiode array detector was developed to analyze the following flavonoids of Rhus verniciflua Stokes: fisetin, fustin, butein, sulfuretin, garbanzol, and quercetin. The optimum conditions for the procedure was the use of Zeolite Socony Mobil-twenty-two molecular sieves as the adsorbent, sample:adsorbent ratio of 2:5, grinding for 3 min, and use of 8 mL of 70% methanol:water as the elution solvent. The method was validated for linearity, precision, reproducibility, limit of detection, and limit of quantification.

gen. nov., sp. nov., a novel cellulose- and xylan-degrading member of the family isolated from a hot spring.

Two anaerobic bacteria, designated strains SYSU GA16112 and SYSU GA16107, were isolated from a hot spring in Tengchong County, Yunnan Province, south-west PR China. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains SYSU GA16112 and SYSU GA16107 belong to the family . Cells of strains SYSU GA16112 and SYSU GA16107 were Gram-stain-negative, rod-shaped and non-motile.

Correction to: Description of Paenibacillus yunnanensis sp. nov., Isolated from a Tepid Spring.

It was brought to our attention that the proposed name Paenibacillus yunnanensis is an illegitimate homonym of Paenibacillus yunnanensis Niu et al. 2015. We therefore propose changing the name of the newly proposed species to Paenibacillus tengchongensis as follows.

Insulin-delivery methods for children and adolescents with type 1 diabetes.

Efforts directed toward restoring normal metabolic levels by mimicking the physiological insulin secretion, thereby ensuring safety, efficacy, minimal invasiveness and conveniences, are of great significance in the management of type 1 diabetes among children and adolescents. Regardless of the various technologies being discovered in addressing invasiveness and enhancing medication adherence in the management of type 1 diabetes, yet limited success had been observed among children and adolescents. The multiple daily subcutaneous insulin injections route using vial and syringe, and occasionally insulin pens, remain the most predictable route for insulin administration among children and adolescents.

Gene expression abundance dictated exopolysaccharide modification in Rhizobium radiobacter SZ4S7S14 as the cell's response to salt stress.

A molecular and metabolic behaviour of EPS-producing and salt-tolerant bacterium Rhizobium radiobacter SZ4S7S14 along with its practical application in salt-stress was investigated. The research target was identification and expression profiles of a large EPS biosynthesis gene cluster, possible structural modification of EPS under salt-stress effect and analysis of the gene(s) relative expression and structural modification correlation. As expected, transposons insertions were identified within or near the coding regions of exoK and exoM, previously known large gene cluster that is required for EPS I synthesis.

From ecophysiology to cultivation methodology: filling the knowledge gap between uncultured and cultured microbes.

Earth is dominated by a myriad of microbial communities, but the majority fails to grow under in situ laboratory conditions. The basic cause of unculturability is that bacteria dominantly occur as biofilms in natural environments. Earlier improvements in the culture techniques are mostly done by optimizing media components.

Enhanced anti-cancer activity of chitosan loaded Morinda citrifolia essential oil against A549 human lung cancer cells.

In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene. The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential. The morphological observation obtained by scanning electron microscopy and transmission electron microscopy provided clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface.

Isolation of Clostridium from Yunnan-Tibet hot springs and description of Clostridium thermarum sp. nov. with lignocellulosic ethanol production.

Lignocellulose is considered a major source of renewable energy that serve as an alternative to the fossil fuels. Members of the genus Clostridium are some of the many microorganisms that have the ability to degrade lignocellulose efficiently to sugar, which can be further converted to biofuel. In this study, we isolated twelve Clostridium strains from hot spring samples of Yunnan and Tibet, of which isolates SYSU GA15002 and SYSU GA17076 showed low 16S rRNA gene sequence identity profiles to any of the validly named Clostridium strains (<94.

Sphingomonas hominis sp. nov., isolated from hair of a 21-year-old girl.

A Gram-stain-negative, aerobic, motile strain, HHU CXW, was isolated from hair of a healthy 21-year-old female student of Hohai University, Nanjing, China. The 16S rRNA gene sequence analysis indicated that HHU CXW represents a member of the genus Sphingomonas with the highest sequence similarity (97.6%) to the type strain S.

Characterization of a Cu, SDS, alcohol and glucose tolerant GH1 β-glucosidase from Bacillus sp. CGMCC 1.16541.

A β-glucosidase gene (bsbgl1a) from Bacillus sp. CGMCC 1.16541 was expressed in Escherichia coli BL21 and subsequently characterized.

Sphingobacterium endophyticum sp. nov., a novel endophyte isolated from halophyte.

A bacterial strain designated NYYP31 was isolated from the leaves of an annual halophytes, Suaeda corniculata Bunge, collected from the southern edge of the Gurbantunggut desert, north-west China. Strain NYYP31 was Gram-staining negative, strictly aerobic, rod-shaped, non-motile, and non-spore-forming. Growth was observed at 4-42 °C, at pH 5.

Deinococcus detaillensis sp. nov., isolated from humus soil in Antarctica.

A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium, designated H1, was isolated from a humus soil sample in the Detaille Island of Antarctica. The 16S rRNA gene sequence result indicated that strain H1 shared the highest 16S rRNA gene sequence identity with the type strain of Deinococcus alpinitundrae (96.2%).

sp. nov., isolated from a deep well with oil reservoir water.

A Gram-stain-negative, rod-shaped bacterium, designated XJ4, was isolated from oil-contaminated water, collected from Xinjiang Province, north-west PR China (45° 1' 27″ N, 85° 6' 14″ E). Growth occurred at 20-45 °C (optimum, 30 °C) and pH 6.0-10.

Bacillus tepidiphilus sp. nov., isolated from tepid spring.

A novel Bacillus strain, designated SYSU G01002, was isolated from a sediment sample collected from tepid spring in Tengchong, Yunnan province, south-west PR China. The 16S rRNA gene sequence analysis showed that the strain SYSU G01002 shared the highest sequence identity with the type strain of Bacillus alkalitolerans (97.7%).

Description of Paenibacillus yunnanensis sp. nov., Isolated from a Tepid Spring.

A Gram stain-positive, rod-shaped and motile bacterium, designated SYSU G01003 was isolated from a sediment sample collected from tepid spring in Tengchong, Yunnan province, southwestern China. Growth observed at temperature ranging 28-37 °C (optimum 37 °C) and pH 6.0-8.