[Measurement and analysis of platycodon grandiflorum with space flight mutagenesis breeding by XRF].

X-ray fluorescence spectrum (XRF) analytic method was used to analyze and compare the contents of various mineral elements in the ground group platycodon grandiflorum, the comparison group platycodon grandiflorum and the fourth generation platycodon grandiflorum with space flight mutagenesis breeding. The result indicated that the contents of microelements Zn, Mn and Fe in the outer space group increase by 1.9, 2.

Clinical application of hepatic CT perfusion.

Complicated changes occur in hemodynamics of hepatic artery and vein, and portal vein under various kinds of pathologic status because of distinct double hepatic blood supply. This article reviews the clinical application of hepatic x-ray computed tomography perfusion in some liver diseases.

[Proteomic analysis in combination with CT diagnosis to distinguish renal cell carcinoma from renal benign masses].

Objective: To identify the proteomic differences between renal cell carcinoma (RCC) and renal benign masses and to evaluate the diagnostic value of parallel and serial test combining with CT and surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS).

Methods: Serum samples were collected from 96 patients with renal tumors, 62 RCC cases and 34 renal benign mass cases, all of which had been evaluated by CT before surgery. The sera were analyzed using IMAC-Cu2+ ProteinChip system by SELDI-TOF-MS.

[ALL 98 strains of Brucella OMP25 gene is conservative in China].

Objective: To investigate the genetic polymorphism of OMP25 gene isolated from 116 Brucella strains, including 18 Brucella reference strains and 98 Chinese field strains.

Methods: Chromosomal DNA of Brucella strains were analyzed by PCR, and then the product OMP25 gene was digested with Hind III and separated on 10% polypropylene agarose gel electrophoresis. OMP25 genes of different types were sequenced.

[Analysis and characterization of Belamcanda chinensis with space mutagenesis breeding by X-ray fluorescence analysis and X-ray diffraction].

The contents of various elements in the fourth generation Belamcanda chinensis (L.) DC. with space mutagenesis breeding were analyzed and characterized.

Proteomic evaluation of urine from renal cell carcinoma using SELDI-TOF-MS and tree analysis pattern.

There is no useful marker in screening and early diagnosis for renal cell carcinomas (RCCs), especially in the urine. To screen for specific markers in the urine of RCCs patients, surface enhanced laser desorption and ionization time of flight mass spectrometry (SELDI-TOF-MS) was used and coupled with a tree analysis pattern to develop SELDI protein profiling of urine. Urine samples from 58 RCC patients, 45 healthy volunteers, and 56 patients with other urogenital diseases were analyzed using IMAC-Cu ProteinChip capable of specifically binding metal interesting proteins.

[Serum proteomic spectra of esophageal squamous cell carcinoma patients analyzed with IMAC3 protein chip].

Background & Objective: Serum protein fingerprinting technology can help to identify the molecular changes related to esophageal carcinogenesis. This study was to screen serum markers and establish the predictive models that may be of help to serologic diagnosis of esophageal squamous cell carcinoma (ESCC).

Methods: Serum samples were collected from 68 ESCC patients and 44 age-and sex-matched healthy subjects, and randomized into a training set (55 ESCC patients and 35 healthy subjects) and a blind testing set (13 ESCC patients and 9 healthy subjects).

[Novel identification of donkeyhide glue by X-ray fluorescence analysis].

A new fast identification method for a Chinese patent medicine donkeyhide glue was established. Six samples from different producing areas were collected and determined by X-ray fluorescence analysis(XRF). Elements characteristic graphs were plotted and compared with those from the comparison sample.

[Serum proteomics study of chronic gastritis with dampness syndrome in traditional Chinese medicine].

Objective: To explore microcosmic information in chronic gastritis dampness syndrome by using serum proteomics of patients with chronic gastritis dampness syndrome.

Methods: Serum proteomics of 18 dampness syndrome cases, 17 non-dampness syndrome cases in chronic gastritis patients and 8 normal controls were analyzed by surface enhanced laser desorption/ionization-time of flight (SELDI-TOF) protein-chip.

Results: There was a high expression trend in three ratios of charge (of an electron) to mass (M/S) of 3.

[Expression of PKD1 and PKD2 transcripts and proteins and its significance in different types of kidney tissues and kidney lines].

Objective: To investigate the expression and function of PKD1 and PKD2 in different kidney tissues and cell lines.

Methods: Immunoprecipitation, Western blotting, In situ hybridization and immunohistochemical staining methods were used to observe the expression of PKD1 mRNA and PKD2 mRNA and their protein abundance in different kidney tissues and cell lines.

Results: Coordinate expressions of PKD1 and PKD2 were found in all kidney tissues and cell lines.

[Screening urine markers of renal cell carcinoma using SELDI-TOF-MS].

Objective: To screen relatively specifical markers in urines from renal cell carcinoma patients using surface-enhanced laser desorption and ionization time of flight mass spectrometry (SELDI-TOF-MS) ProteinChip technology.

Methods: Urine samples from 40 renal cell carcinoma (RCC) patients, 40 healthy volunteers and 40 patients with other urogenital diseases were analyzed using IMAC-Cu-3 PoteinChip, which can specifically bind the metal-combining-proteins. Proteomic spectra were generated by mass spectrometry.

[Detection of fetal SRY gene in maternal plasma by real-time fluorescence quantitative PCR].

Objective: To isolate fetal DNA from maternal plasma and examine its fetal origin.

Methods: Fetal DNA in maternal plasma was isolated from 150 samples in the first trimester and mid-trimester of pregnancy, respectively. Real-time fluorescence quantitative polymerase chain reaction PCR (FQ-PCR) was used to determine sex-determining region Y (SRY) gene on Y chromosome.

Deletion of exon 4 from human surfactant protein C results in aggresome formation and generation of a dominant negative.

Human surfactant protein C (hSP-C) is synthesized by the alveolar type 2 cell as a 197 amino acid integral membrane proprotein and proteolytically processed to a secreted 3.7 kDa mature form. Although the SP-C null mouse possesses a non-lethal phenotype, a heterozygous substitution of A for G in the first base of intron 4 of the human SP-C gene (c.

Biosynthesis of surfactant protein C (SP-C). Sorting of SP-C proprotein involves homomeric association via a signal anchor domain.

Rat surfactant protein C (SP-C) is synthesized as a 194-amino acid propeptide (SP-C-(1-194)) that is directed to the distal secretory pathway and proteolytically processed as an integral membrane protein to yield its mature form. We had shown previously that trafficking of proSP-C is mediated both by a signal anchor domain contained within the mature SP-C sequence and by a targeting domain in the NH(2)-flanking propeptide. Based on evidence from other integral membrane proteins, we hypothesized that proSP-C targeting is effected by oligomerization of proSP-C monomers.