Production of biomass and recombinant human-like collagen in Escherichia coli processes with different CO2 pulses.

The evolution of CO(2) in a fed-batch culture of recombinant Escherichia coli containing human-like collagen (HLC) cDNA was determined with an O(2)-enriched air supply (40%, v/v) in a 12.8 l fermentor; a maximum CO(2) concentration of 12.7% in the effluent gas was detected.