A Novel 10-Base Pair Deletion in the First Exon of Promotes Hypocotyl Elongation, Induces Early Maturation, and Impairs Photosynthetic Performance in Soybean.

Plants photoreceptors perceive changes in light quality and intensity and thereby regulate plant vegetative growth and reproductive development. By screening a γ irradiation-induced mutant library of the soybean () cultivar "Dongsheng 7", we identified , a mutant with elongated nodes, yellowed leaves, decreased chlorophyll contents, altered photosynthetic performance, and early maturation. An analysis of bulked DNA and RNA data sampled from a population segregating for , using the BVF-IGV pipeline established in our laboratory, identified a 10 bp deletion in the first exon of the candidate gene .

Synthesis of -linked α-Gal and α-GalNAc-1'-hydroxyalkanes by way of C2 functionality transfer.

Inspired by reports of water sculpted Tn antigen (α-GalNAc--Ser/Thr) epitopes and our interest in producing metabolically more stable -linked analogs of Tn, we explored the utility of C2 functionality on α-Gal--alkenes to deliver hydroxyl to the pendant alkenyl chain. Toward this end, a cyclic carbonate approach gave rise to a single -linked α-Gal-1'(S)-hydroxyethane in 3 steps, and use of a 2-(hydroxyimino)galactoside cyclization transferred an oxygen to a pendant -substituted -linked alkene affording the -configuration at the newly formed stereocenter (7:1 dr). Reduction and acetylation of the resultant isoxazoline demonstrated this approach as a viable route to -linked α-GalNAc-1'-hydroxyalkanes.