Zygotic genome activation occurs in two-cell (2C) embryos, and a 2C-like state is also activated in sporadic (~1%) naïve embryonic stem cells in mice. Elevated chromatin accessibility is critical for the 2C-like state to occur, yet the underlying molecular mechanisms remain elusive. Zscan4 exhibits burst expression in 2C embryos and 2C-like cells.
View Article and Find Full Text PDFBackground: Fascioliasis is a zoonotic parasitic disease caused by Fasciola hepatica and Fasciola gigantica, which poses a serious threat to global public health and livestock farming. Fasciola gigantica secretes and excretes various components to manipulate the immune response, thereby enhancing its invasion, migration, and survival in vivo. However, the roles of specific components in immune modulation, such as asparagine endopeptidase, remain unknown.
View Article and Find Full Text PDFMedicine (Baltimore)
December 2024
Kidney stones formation is a multifactorial condition and influenced, in some degree, by dietary habits. Authoritative clinical guidelines published nutritional recommendations for individuals prone to kidney stone formation. The association between dietary intake calcium to phosphorus (Ca/P) ratios and the prevalence of kidney stones is not well-established in extensive epidemiological studies.
View Article and Find Full Text PDFEur J Obstet Gynecol Reprod Biol
December 2024
Introduction: Interstitial Cystitis/Bladder Pain Syndrome (IC/BPS) is a chronic and debilitating condition marked by bladder pain, urinary urgency, and frequency. The pathophysiology of IC/BPS remains poorly understood, with limited therapeutic options available. The role of Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) and its receptor PAC1 in IC/BPS has not been thoroughly investigated, despite their potential involvement in inflammation and sensory dysfunction.
View Article and Find Full Text PDFThe development of mitochondria-targeting nanozymes holds significant promise for treating myocardial ischemia-reperfusion (IR) injury but faces significant biological barriers. To overcome these obstacles, we herein utilized genetically engineered ferritin nanocages (i.e.
View Article and Find Full Text PDF-methylpyrrolidone (NMP) is an excellent advanced solvent that can be easily absorbed by the human body and has the characteristics of flammability and explosion. To reduce the risk, the environmental concentrations of NMP need to be measured. A series of covalent organic frameworks (COF) connected by an imine bond have been successfully prepared at room temperature by changing the synthesis time catalyzed by scandium(III) trifluoromethanesulfonate (Sc(OTf)).
View Article and Find Full Text PDFIn order to effectively control the large deformation of coal pillar and roadway during entry retained along gob side with small coal pillar, taking 1311 and 1312 working faces of a coal mine in Shanxi Province as engineering background, the structural characteristics of overlying rock are studied, and the stress source of entry retained along gob side with small coal pillar and the principle of roof cutting and pressure relief are analyzed, then the roof cutting and pressure relief technology of shaped charge blasting with combined and grouped of deep and shallow holes is put forward. According to the geological conditions of 1311 working face, through the calculation of caving zone and crack zone height of the working face, the analysis of roof breaking and instability conditions and the calculation of crack zone blasting radius, the key parameters of roof cutting by deep and shallow hole combined blasting are determined. Through the numerical simulation and analysis of roof cutting and pressure relief in different key strata, the variation law of vertical stress acting on small coal pillar is obtained, as follows: no roof cutting > only cut layer 2 > only cut layer 1 > cut layer 3 + partial layer 2 > cut layer 1 ~ 3; Based on these, a cooperative control scheme of "roof cutting and pressure relief by shaped charge blasting with combined and grouped of deep and shallow holes" + "anchor-cable combined support" + "coal pillar strengthened" is put forward.
View Article and Find Full Text PDF() is a zoonotic bacterial pathogen that causes chronic host infections. The eradication of brucellosis using antibiotic therapy is often incomplete or slow. In a mouse model, the predominance of alternatively activated macrophages (also known as M2) plays an essential role in sustaining chronic infection.
View Article and Find Full Text PDFHutchinson-Gilfor progeria syndrome (HGPS) is caused by a mutation in Lamin A resulting in the production of a protein called progerin. The accumulation of progerin induces inflammation, cellular senescence and activation of the P53 pathway. In this study, through public dataset analysis, we identified Syntaxin Binding Protein 5 (STXBP5) as an influencing factor of progerin expression.
View Article and Find Full Text PDFTo investigate the effect of 5-aminolevulinic acid (5-ALA) on in vitro rumen gas production, fermentation characteristics, and bacterial community profiles, five levels of 5-ALA (0, 100, 500, 1000, and 5000 mg/kg DM) were supplemented into a total mixed ration (concentrate/forage = 40:60) as substrate in an in vitro experiment. Results showed that as the supplementation level of 5-ALA increased, asymptotic gas production (b) decreased linearly and quadratically ( < 0.01) while the dry matter degradation rate increased quadratically ( < 0.
View Article and Find Full Text PDFHuan Jing Ke Xue
September 2024
This study investigated the impact of single and combined applications of three foliar inhibitors on the accumulation of cadmium (Cd) and arsenic (As) in rice grains. Two rice varieties, Songyazao 1 (for early rice) and Wuxiang Youyue (for late rice), were selected for this experiment. We established nine treatments using a pot experiment method, including a control (CK) treated with no foliar inhibitor and three individual foliar inhibitors: cysteine (L-Cys), potassium sulfide (KS), and dipotassium hydrogen phosphate (KHPO).
View Article and Find Full Text PDFMetabolic reprogramming is pivotal in cancer stem cell (CSC) self-renewal. However, the intricate regulatory mechanisms governing the crosstalk between metabolic reprogramming and liver CSCs remain elusive. Here, using a metabolic CRISPR-Cas9 knockout screen, we identify ATP6V1D, a subunit of the vacuolar-type H-translocating ATPase (V-ATPase), as a key metabolic regulator of hepatocellular carcinoma (HCC) stemness.
View Article and Find Full Text PDFPhosphoric acid (PA) leakage and volume expansion are critical factors limiting long-term stable operation of PA-doped polybenzimidazole (PBI) for high-temperature proton exchange membrane fuel cells. Enhancing the interaction between the polymer matrix and PA provides an effective way to minimize PA loss and inhibit excessive membrane swelling. The covalent organic frameworks (COFs) are helpful in improving the performance of PA-PBI membranes due to the robust frameworks, adjustable structures, and good compatibility with polymers.
View Article and Find Full Text PDFBackground: The resistance to endocrine therapy can lead to recurrence and metastasis of breast cancer (BC), affecting the survival period. Tiaogan Bushen Xiaoji (TGBSXJ) Formula, a traditional Chinese medicine (TCM) decoction, has been widely used in the treatment of estrogen receptor-positive (ER) BC. However, the underlying mechanism of TGBSXJ Formula in ERBC treatment has not been totally elucidated.
View Article and Find Full Text PDFBuffaloes cannot mount a robust adaptive immune response to secondary infection by Fasciola gigantica. Even if excretory and secretory products (ESPs) exhibit potent immunoregulatory effects during primary infection, research on ESPs in secondary infection is lacking, even though the ESP components that are excreted/secreted during secondary infection are unknown. Therefore, qualitative analysis of ESP during secondary infection was performed and compared with that of primary infection to deepen the recognition of secondary infection and facilitate immunoregulatory molecules screening.
View Article and Find Full Text PDFObjectives: To estimate the number of pregnancies complicated by vasa previa annually in nine developed countries, and the potential preventable stillbirths associated with undiagnosed cases. We also assessed the potential impact of universal screening for vasa previa on reducing stillbirth rates.
Methods: We utilized nationally-reported birth and stillbirth data from public databases in the United States, United Kingdom, Canada, Germany, Ireland, Greece, Sweden, Portugal, and Australia.
Objective: The prevalence of Lower Urinary Tract Symptoms suggestive of Benign Prostatic Hyperplasia (LUTS/BPH) is notably high and potentially elevates the likelihood of depressive symptoms. This study was designed to employ both cross-sectional and longitudinal methodologies to explore the correlation between LUTS/BPH and depressive symptoms among middle-aged and elderly men in China.
Methods: This investigation utilized data from the China Health and Retirement Longitudinal Study (CHARLS), with the initial dataset from 2015 serving as the baseline and subsequent data from 2018 and 2020 facilitating longitudinal analysis.
Beijing Da Xue Xue Bao Yi Xue Ban
December 2024
Objective: To explore the clinical safety and effectiveness of self-draining ureteral stent with thread in kidney transplant recipients in renal transplantation.
Methods: This study is a prospective cohort clinical study in the Department of Urology of Peking University People's Hospital from November 2022 to January 2024. The ureteral stent with thread group, in which a 2-0 Mersilene suture of 20-30 cm was used at the bladder end of the ureteral stent during the operation.
Background: The insulin/insulin-like signalling (IIS) pathway is common in mammals and invertebrates, and the IIS pathway is unknown in Fasciola gigantica. In the present study, the IIS pathway was reconstructed in F. gigantica.
View Article and Find Full Text PDFAs a sustainable and promising approach of removing of nitrogen oxides (NO), catalytic reduction of NO with H is highly desirable with a precise understanding to the structure-activity relationship of supported catalysts. In particular, the dynamic evolution of support at microscopic scale may play a critical role in heterogeneous catalysis, however, identifying the in situ structural change of support under working condition with atomic precision and revealing its role in catalysis is still a grand challenge. Herein, we visually capture the surface lattice expansion of WO support in Pt-WO catalyst induced by NO in the exemplified reduction of NO with H using in situ transmission electron microscopy and first reveal its important role in enhancing catalysis.
View Article and Find Full Text PDFThe dysregulation of membrane protein expression has been implicated in tumorigenesis and progression, including hepatocellular carcinoma (HCC). In this study, we aimed to identify membrane proteins that modulate HCC viability. To achieve this, we performed a CRISPR activation screen targeting human genes encoding membrane-associated proteins, revealing TMX2 as a potential driver of HCC cell viability.
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