miRNA-125a regulates porcine oocyte maturation in vitro by targeting ADAR.

Follicular fluid extracellular vesicles are beneficial for in vitro oocyte maturation and development; however, their effect on the expression profiles of oocyte microRNAs (miRNAs) and the roles of related miRNAs are unknown. In this study, we aimed to investigate miRNA expression in mature oocytes cultured in follicular fluid extracellular vesicles and the effect of miRNA-125a (miR-125a) on oocyte maturation. The expression profiles of the miRNAs were determined by microRNA sequencing, followed by target gene prediction analysis.

Unsupervised deep learning-based medical image registration: a survey.

In recent decades, medical image registration technology has undergone significant development, becoming one of the core technologies in medical image analysis. With the rise of deep learning, deep learning-based medical image registration methods have achieved revolutionary improvements in processing speed and automation, showing great potential, especially in unsupervised learning. This paper briefly introduces the core concepts of deep learning-based unsupervised image registration, followed by an in-depth discussion of innovative network architectures and a detailed review of these studies, highlighting their unique contributions.

Follicular fluid exosome-derived miR-339-5p enhances in vitro maturation of porcine oocytes via targeting SFPQ, a regulator of the ERK1/2 pathway.

In this study, we aimed to investigate cytoplasmic maturation and miRNA expression of mature oocytes cultured in porcine follicular fluid exosomes. We also examined the effect of miR-339-5p on oocyte maturation. Twenty eight differentially expressed miRNAs were detected using miRNA-seq.

[Clinical observation on acupuncture combined with warming acupuncture for postoperative urinary retention].

Objective: To compare the therapeutic effect of acupuncture (acupuncture for unblocking governor vessel and regulating ) combined with warming acupuncture, acupuncture, abdominal moxibustion and oral tamsulosin hydrochloride sustained release capsule on postoperative urinary retention.

Methods: A total of 120 patients with postoperative urinary retention were randomized into an acupuncture-moxibustion group, an acupuncture group, a moxibustion group and a medication group, 30 cases in each group. acupuncture combined with warming acupuncture were applied in the acupuncture-moxibustion group.

Synergistic effects of low‑dose chemotherapy and T cells in renal cell carcinoma.

Renal cell carcinoma (RCC) is not sensitive to conventional radiotherapy and chemotherapy, and the effectiveness rate of molecular targeted therapy is low. Therefore, it is urgent to identify new treatment methods. Recently, adoptive T‑cell therapy has provided a new option for cancer treatment.

The development of CAR design for tumor CAR-T cell therapy.

In recent years, the chimeric antigen receptor modified T cells (Chimeric antigen receptor T cells, CAR-T) immunotherapy has developed rapidly, which has been considered the most promising therapy. Efforts to enhance the efficacy of CAR-based anti-tumor therapy have been made, such as the improvement of structures of CAR-T cells, including the development of extracellular antigen recognition receptors, intracellular co-stimulatory molecules and the combination application of CARs and synthetic small molecules. In addition, effects on the function of the CAR-T cells that the space distance between the antigen binding domains and tumor targets and the length of the spacer domains have are also being investigated.