A rapid and sensitive one-pot platform integrating fluorogenic RNA aptamers and CRISPR-Cas13a for visual detection of monkeypox virus.

The recent global upsurge in Monkeypox virus (MPXV) outbreaks underscores the critical need for rapid and precise diagnostic solutions, particularly in resource-constrained settings. The gold standard diagnostic method, qRT-PCR, is hindered by its time-consuming nature, requirement for nucleic acid purification, expensive equipment, and the need for highly trained personnel. Traditional CRISPR/Cas fluorescence assays, relying on trans-cleavage of ssDNA/RNA reporters labeled with costly fluorophores and quenchers, pose challenges that limit their widespread application, especially for point-of-care testing (POCT).

Prevalence of strabismus among preschool children in eastern China and comparison at a 5-year interval: a population-based cross-sectional study.

Objective: To update data on strabismus and evaluate the changes in prevalence and patterns among preschoolers in eastern China over a period of 5 years.

Design: Nanjing Eye Study, a longitudinal population-based study.

Setting: Recruitment and testing in kindergartens in Yuhuatai District, Nanjing.

CARF, As An Oncogene, Promotes Colorectal Cancer Stemness By Activating ERBB Signaling Pathway.

Introduction: The role of CARF, a calcium-responsive transcription factor, in colorectal cancer initiation and development is still unknown. Here, we report that CARF promotes colorectal cancer stemness through ERBB signaling pathway.

Materials And Methods: Both colorectal cancer cell lines and primary cells were used in this study.