Engineered mRNAs With Stable Structures Minimize Double-stranded RNA Formation and Increase Protein Expression.

The therapeutic use of synthetic message RNA (mRNA) has been validated in COVID-19 vaccines and shows enormous potential in developing infectious and oncological vaccines. However, double-stranded RNA (dsRNA) byproducts generated during the in vitro transcription (IVT) process can diminish the efficacy of mRNA-based therapeutics and provoke innate immune responses. Existing methods to eliminate dsRNA byproducts are often cumbersome and labor-intensive.

Structure-dependent magnetoelectric and magnetothermal effects of MOF-derived zero-valence cobalt and iron oxide nanoparticles on a carbonaceous matrix.

For the first time, the dominant magnetoelectric activity of ZIF-67-derived carbonaceous microparticles embedded with Co nanoparticles and distinctive magnetothermal effect of MIL-88B-derived FeO nanocubes decorated on carbonaceous microrods, respectively, were explored to be controlled by the structure of the MOF-derived electrically conductive carbonaceous matrix and metal nanoparticles.

Artificial Intelligence-Based Facial Palsy Evaluation: A Survey.

Facial palsy evaluation (FPE) aims to assess facial palsy severity of patients, which plays a vital role in facial functional treatment and rehabilitation. The traditional manners of FPE are based on subjective judgment by clinicians, which may ultimately depend on individual experience. Compared with subjective and manual evaluation, objective and automated evaluation using artificial intelligence (AI) has shown great promise in improving traditional manners and recently received significant attention.

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization.

The cellular affinity of micro-/nanoparticles is the precondition for cellular recognition, cellular uptake, and activation, which are essential for drug delivery and immune response. The present study stemmed from the observation that the effects of charge, size, and shape of solid particles on cell affinity are usually considered, but we seldom realize the essential role of softness, dynamic restructuring phenomenon, and complex interface interaction in cellular affinity. Here, we developed poly-lactic-co-glycolic acid (PLGA) nanoparticle-stabilized Pickering emulsion (PNPE) that overcame the shortcomings of rigid forms and simulated the flexibility and fluidity of pathogens.

Humidity-Tolerant Chemiresistive Gas Sensors Based on Hydrophobic CeO/SnO Heterostructure Films.

The accelerated evolution of the Internet of Things has brought new challenges to the gas sensors, which are required to work persistently under harsh conditions, like high humidity. However, currently, it is quite challenging to solve the hindrance of the trade-off between gas-sensing performance and anti-humidity ability of the chemiresistive gas sensors. Herein, hydrophobic inorganic CeO/SnO heterostructure films were prepared by depositing the CeO layers with a thickness of a few nanometers onto the SnO film via a magnetron sputtering method.

Fine mapping and conservation analysis of linear B-cell epitopes of peste des petits ruminants virus hemagglutinin protein.

Hemagglutinin protein (H), one of the two glycoproteins of peste des petits ruminants virus (PPRV), binds to its receptor on the host cell and acts as a major antigen that induces and confers highly protective immunity in the host. In order to delineate the epitopes on H protein, fine epitope mapping and conservation analysis of linear B-cell epitopes (BCEs) on PPRV H has been undertaken using biosynthetic peptides and rabbit anti-PPRV H sera. Thirteen linear BCEs were identified and their corresponding minimal motifs were located on the H protein of PPRV China/Tibet/Geg/07-30.