Identifying key genes involved in yellow leaf variation in 'Menghai Huangye' based on biochemical and transcriptomic analysis.

Albino tea plants generally have higher theanine, which causes their tea leaves to taste fresher, and they are an important mutant for the breeding of tea plant varieties. Earlier, we reported an albino germplasm, 'Menghai Huangye' (MHHY), from Yunnan Province and found that it has a lower chlorophyll content during the yellowing stage, but the mechanism underlying low chlorophyll and the yellowing phenotype is still unclear. In this study, the pigment contents of MHHY_May (yellowing, low chlorophyll), MHHY_July (regreening, normal chlorophyll), and YK10_May (green leaves, normal chlorophyll) were determined, and the results showed that the lower chlorophyll content might be an important reason for the formation of the yellowing phenotype of MHHY.

Identification and characterization of miRNAs associated with sterile flower buds in the tea plant based on small RNA sequencing.

Background: miRNAs are a type of conserved, small RNA molecule that regulate gene expression and play an important role in the growth and development of plants. miRNAs are involved in seed germination, root development, shoot apical meristem maintenance, leaf development, and flower development by regulating various target genes. However, the role of miRNAs in the mechanism of tea plant flower sterility remains unclear.

[Effect of chronic stress on PKA and P-CREB expression in hippocampus of rats and the antagonism of antidepressors].

Objective: To observe the effect of chronic unpredicted sequence of mild stress on the expression of cAMP-dependent protein kinase A(PKA) and phosphorylated cAMP-responsive element binding protein (P-CREB) in hippocampus of rats and the antagonism of antidepressors (fluoxetine).

Methods: Thirty-six male Sprague Dawley rats were randomly and equally allocated to 3 groups: A normal control group, a model group, and a fluoxetine group. All rats except the control group were singly housed and exposed to an unpredicted sequence of mild stressors.

[Effects of baisong tablets on the behavior and levels of norepinephrine and dopamine in the brain of stress rats].

Objective: To explore the effects of baisong tablets on the behavior and contents of norepinephrine and dopamine in the brain of stress rats.

Methods: Forty adult Sprague-Dawley male rats were randomly divided into 4 groups: the control group, the depression model group, the baisong tablet group and the fluoxetine group. The depression model was replicated by chronic unpredictable mild stress and single house in 21 days.