In an era marked by unprecedented anthropogenic change, marine systems are increasingly subjected to interconnected and dynamic external stressors, which profoundly reshape the behavior and resilience of marine ecological components. Nevertheless, despite widespread recognition of the significance of stressor interactions, there persist notable knowledge deficits in quantifying their interactions and the specific biological consequences that result. To bridge this crucial gap, this research detected and examined the causal relationships between five key exogenous stressors in a complex estuarine ecosystem.
View Article and Find Full Text PDFA novel CD19xCD3xCD28 trispecific antibody with a tandem single-chain variable fragments (scFv) structure was developed for the treatment of B-cell malignancies. The trispecific antibody in inducing tumor-directed T-cell activation and cytotoxicity was evaluated and and compared with its bispecific counterpart BiTE-CD19xCD3 lacking a CD28-targeting domain. The trispecific antibody with a co-stimulatory domain exhibited augmented T-cell activation and memory T-cell differentiation capability and it induced faster tumor cell lysis than the bispecific antibody.
View Article and Find Full Text PDFThe association of neurogenesis and gliogenesis with glioma remains unclear. By conducting single-cell RNA-seq analyses on 26 gliomas, we reported their classification into primitive oligodendrocyte precursor cell (pri-OPC)-like and radial glia (RG)-like tumors and validated it in a public cohort and TCGA glioma. The RG-like tumors exhibited wild-type isocitrate dehydrogenase and tended to carry EGFR mutations, and the pri-OPC-like ones were prone to carrying TP53 mutations.
View Article and Find Full Text PDFThe Formosa Ridge, also named Site F, is an active cold seep marine ecosystem site that has been studied since it was discovered on the continental slope of the northeast South China Sea (SCS). However, few studies have focused on the eukaryotic diversity at Site F. Environmental DNA (eDNA) technology is a non-invasive method applied in biodiversity surveys with a high species detection probability.
View Article and Find Full Text PDFChelidonichthys spinosus, a secondary economic fish, is increasingly being exploited and valued in China. However, overfishing has led to it being recognized as one of the most depleted marine species in China. In this study, we generated a chromosome-level genome of C.
View Article and Find Full Text PDFThe big-head schizothorcin (Aspiorhynchus laticeps) is an endemic and near-extinction freshwater fish in Xinjiang, China. In this study, a chromosome-scale genome assembly of A. laticeps was generated using PacBio and Hi-C techniques.
View Article and Find Full Text PDFThe prevailing determinant of maturation in fishes is thought to be a redirection of energy from growth to reproduction. Instead, the Gill Oxygen Limitation Theory predicts that maturation, and thus reproduction, is induced when a fish reaches a critical ratio of oxygen supply to demand (Q /Q ). The consistency of this critical ratio has been previously documented in many fishes, but a broader test was lacking.
View Article and Find Full Text PDFThe South China Sea (SCS) is an important part of the Indo-Pacific convergence zone, with high biodiversity and abundant marine resources. Traditional methods are primarily used to monitor biodiversity. However, a few studies have used environmental DNA (eDNA) metabarcoding to research the assemblage structure of the SCS.
View Article and Find Full Text PDFPatients with monoallelic bromodomain and PHD finger-containing protein 1 () mutations showed intellectual disability. The hippocampus has essential roles in learning and memory. Our previous work indicated that was specifically and strongly expressed in the hippocampus from the perinatal period to adulthood.
View Article and Find Full Text PDFMitochondrial DNA B Resour
August 2021
The complete mitochondrial genome of the half-fin anchovy, collected from Yellow and Bohai Seas was determined by next-generation sequencing. The mitogenome is a circular molecule 16,668 bp in length, including the typical structure of 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a control region. The termination-associated sequence (TAS), central conserved sequence block (CSB) and CSB are detected in the control region.
View Article and Find Full Text PDFMitochondrial DNA B Resour
March 2021
The spiny red gurnard, is a common marine economic fish species along the coast of China. In the present study, the complete mitochondrial genome of spiny red gurnard collected from the Yangtze Estuary was determined by next-generation sequencing (NGS). The mitogenome is a circular nucleotide 16,466 bp in length and has the typical vertebrate genome structure of 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and two control regions (Origin of Heavy chain and Origin of Light chain).
View Article and Find Full Text PDFIntellectual disability is closely related to impaired GABA neurotransmission. Brpf1 was specifically expressed in medial ganglionic eminence (MGE), a developmental niche of GABAergic interneurons, and patients with BRPF1 mutations showed intellectual disability. To test its role in the development and function of MGE-derived GABAergic interneurons, we performed immunofluorescence staining, whole-cell patch-clamp, MGE transplantation, and mRNA-Seq to understand its effect on neuronal differentiation, dendritic morphology, electrophysiology, migration, and gene regulation, using mouse MGE-derived GABAergic interneurons infected with AAV-shBrpf1.
View Article and Find Full Text PDFMitochondrial DNA B Resour
February 2021
The complete mitochondrial genome of collected from Yellow and Bohai Seas was determined by next-generation sequencing. The mitogenome is a circular molecule 16,513 bp in length, including the typical structure of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and a control region. The TAS, central CSB, and CSB were detected in the control region.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
September 2020
The ecological niche is a key concept for elucidating patterns of species distributions and developing strategies for conserving biodiversity. However, recent times are seeing a widespread debate whether species niches are conserved across space and time (niche conservatism hypothesis). Biological invasions represent a unique opportunity to test this hypothesis in a short time frame at the global scale.
View Article and Find Full Text PDFThe reliability of transferring species distribution models (SDMs) to new ranges and future climates has been widely debated. Biological invasions offer the unique opportunity to evaluate model transferability, as distribution data between species' native and introduced ranges are geographically independent of each other. Here, we performed the first global quantitative synthesis of the spatial transferability of SDMs for 235 invasive species and assessed the association of model transferability with the focal invader, model choice and parameterisation.
View Article and Find Full Text PDFSeasonal variations of the ichthyoplankton assemblage and its relationship with the environment were analyzed based on four seasonal surveys during 2012. Historical data was collected to be compared with results from previous years in order to indicate the seasonal and inter-annual variation of the ichthyoplankton assemblage in the Yangtze Estuary and the adjacent waters. A total of 3,688 individuals belonging to 5 orders, 9 families, and 15 species were collected.
View Article and Find Full Text PDFThe rearrangement and expression of immunoglobulin genes are regulated by enhancers and their binding transcriptional factors that activate or suppress the activities of the enhancers. The immunoglobulin κ (Igκ) gene locus has three important enhancers: the intrinsic enhancer (Ei), 3' enhancer (E3'), and distal enhancer (Ed). Ei and E3' are both required for Igκ gene rearrangement during early stages of B-cell development, whereas optimal expression of the rearranged Igκ gene relies on both E3' and Ed.
View Article and Find Full Text PDFResources of Japanese anchovy ( Temminck & Schlegel, 1846) are undergoing dramatic recessions in China as the consequence of intensifying anthropogenic activities. Elucidating the influences of local-scale environmental factors on early life history traits is of great importance to design strategies conserving and restoring the declining anchovy resources. In this research, we studied hatching date and early growth of anchovy in the Yangtze River Estuary (YRE) using information obtained from otolith microstructure.
View Article and Find Full Text PDFThe complete mitochondrial genome of the larvae collected from Yangtze estuary was determined by next-generation sequencing. The mitogenome is a circular molecule 16,536 bp in length, including the typical structure of 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a control region. The TAS, central CSB and CSB were detected in the control region.
View Article and Find Full Text PDFThe complete mitochondrial genome of the larval collected from Yangtze estuary was determined by next-generation sequencing. The mitogenome is a circular molecule 16,465 bp in length, including 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a control region. The TAS, central CSB and CSB were detected in the control region.
View Article and Find Full Text PDFThe contraction and relaxation events of the muscle is mediated by the coordination of many important calcium cycling proteins of ryanodine receptor (RYR), troponin C (TNNC), parvalbumin (PVALB), sarcoendoplasmic reticulum calcium transport ATPase (SERCA) and calsequestrin (CASQ). In higher vertebrates, the expression level of calcium cycling proteins are positively correlated to the muscle contraction/relaxation ability of the cell. In this study, we used RNAseq to explore the expression profile of calcium cycling genes between two marine fish of Bombay duck () and beltfish () with poor and robust swimming activities, respectively.
View Article and Find Full Text PDFThe beltfish (Trichiurus lepturus) is considered as one of the most economically important marine fish in East Asia. It is a top predator with a robust swimming ability that is a good model to study muscle physiology in fish. In the present study, we used Illumina sequencing technology (NextSeq500) to sequence, assemble and annotate the muscle transcriptome of juvenile beltfish.
View Article and Find Full Text PDFThis study investigated the response of the ichthyoplankton community to environmental changes in the Yangtze Estuary using canonical correspondence analysis. Ichthyoplankton community and environmental data were recorded during the autumns of 1998, 2000, 2002, 2003, 2004, 2007 and 2009. Among the ichthyoplankton, the dominant larval and juvenile families were the Engraulidae, Gobiidae and Salangidae, and the most common eggs were from Trichiurus lepturus.
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