Interactions of multiple stressors on the Bombay-duck Harpadon nehereus population in a complex estuarine ecosystem.

In an era marked by unprecedented anthropogenic change, marine systems are increasingly subjected to interconnected and dynamic external stressors, which profoundly reshape the behavior and resilience of marine ecological components. Nevertheless, despite widespread recognition of the significance of stressor interactions, there persist notable knowledge deficits in quantifying their interactions and the specific biological consequences that result. To bridge this crucial gap, this research detected and examined the causal relationships between five key exogenous stressors in a complex estuarine ecosystem.

A trispecific antibody induces potent tumor-directed T-cell activation and antitumor activity by CD3/CD28 co-engagement.

A novel CD19xCD3xCD28 trispecific antibody with a tandem single-chain variable fragments (scFv) structure was developed for the treatment of B-cell malignancies. The trispecific antibody in inducing tumor-directed T-cell activation and cytotoxicity was evaluated and and compared with its bispecific counterpart BiTE-CD19xCD3 lacking a CD28-targeting domain. The trispecific antibody with a co-stimulatory domain exhibited augmented T-cell activation and memory T-cell differentiation capability and it induced faster tumor cell lysis than the bispecific antibody.

Distinct immune escape and microenvironment between RG-like and pri-OPC-like glioma revealed by single-cell RNA-seq analysis.

The association of neurogenesis and gliogenesis with glioma remains unclear. By conducting single-cell RNA-seq analyses on 26 gliomas, we reported their classification into primitive oligodendrocyte precursor cell (pri-OPC)-like and radial glia (RG)-like tumors and validated it in a public cohort and TCGA glioma. The RG-like tumors exhibited wild-type isocitrate dehydrogenase and tended to carry EGFR mutations, and the pri-OPC-like ones were prone to carrying TP53 mutations.

Biodiversity exploration of Formosa Ridge cold seep in the South China Sea using an eDNA metabarcoding approach.

The Formosa Ridge, also named Site F, is an active cold seep marine ecosystem site that has been studied since it was discovered on the continental slope of the northeast South China Sea (SCS). However, few studies have focused on the eukaryotic diversity at Site F. Environmental DNA (eDNA) technology is a non-invasive method applied in biodiversity surveys with a high species detection probability.

Chromosome genome assembly and annotation of the spiny red gurnard (Chelidonichthys spinosus).

Chelidonichthys spinosus, a secondary economic fish, is increasingly being exploited and valued in China. However, overfishing has led to it being recognized as one of the most depleted marine species in China. In this study, we generated a chromosome-level genome of C.

Chromosomal-scale genome assembly of the near-extinction big-head schizothorcin (Aspiorhynchus laticeps).

The big-head schizothorcin (Aspiorhynchus laticeps) is an endemic and near-extinction freshwater fish in Xinjiang, China. In this study, a chromosome-scale genome assembly of A. laticeps was generated using PacBio and Hi-C techniques.

The ratio of length at first maturity to maximum length across marine and freshwater fishes.

The prevailing determinant of maturation in fishes is thought to be a redirection of energy from growth to reproduction. Instead, the Gill Oxygen Limitation Theory predicts that maturation, and thus reproduction, is induced when a fish reaches a critical ratio of oxygen supply to demand (Q /Q ). The consistency of this critical ratio has been previously documented in many fishes, but a broader test was lacking.

Biodiversity exploration in autumn using environmental DNA in the South China sea.

The South China Sea (SCS) is an important part of the Indo-Pacific convergence zone, with high biodiversity and abundant marine resources. Traditional methods are primarily used to monitor biodiversity. However, a few studies have used environmental DNA (eDNA) metabarcoding to research the assemblage structure of the SCS.

Deficiency of Intellectual Disability-Related Gene Attenuated Hippocampal Excitatory Synaptic Transmission and Impaired Spatial Learning and Memory Ability.

Patients with monoallelic bromodomain and PHD finger-containing protein 1 () mutations showed intellectual disability. The hippocampus has essential roles in learning and memory. Our previous work indicated that was specifically and strongly expressed in the hippocampus from the perinatal period to adulthood.

The complete mitochondrial genome of the half-fin anchovy, (Valenciennes, 1848).

The complete mitochondrial genome of the half-fin anchovy, collected from Yellow and Bohai Seas was determined by next-generation sequencing. The mitogenome is a circular molecule 16,668 bp in length, including the typical structure of 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a control region. The termination-associated sequence (TAS), central conserved sequence block (CSB) and CSB are detected in the control region.

The complete mitochondrial genome of the spiny red gurnard McClelland, 1844 (Scorpaeniformes: Triglidae).

The spiny red gurnard, is a common marine economic fish species along the coast of China. In the present study, the complete mitochondrial genome of spiny red gurnard collected from the Yangtze Estuary was determined by next-generation sequencing (NGS). The mitogenome is a circular nucleotide 16,466 bp in length and has the typical vertebrate genome structure of 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and two control regions (Origin of Heavy chain and Origin of Light chain).

Deficiency of intellectual disability-related gene Brpf1 reduced inhibitory neurotransmission in MGE-derived GABAergic interneurons.

Intellectual disability is closely related to impaired GABA neurotransmission. Brpf1 was specifically expressed in medial ganglionic eminence (MGE), a developmental niche of GABAergic interneurons, and patients with BRPF1 mutations showed intellectual disability. To test its role in the development and function of MGE-derived GABAergic interneurons, we performed immunofluorescence staining, whole-cell patch-clamp, MGE transplantation, and mRNA-Seq to understand its effect on neuronal differentiation, dendritic morphology, electrophysiology, migration, and gene regulation, using mouse MGE-derived GABAergic interneurons infected with AAV-shBrpf1.

The complete mitochondrial genome of ribbed gunnel (van der Hoeven, 1855).

The complete mitochondrial genome of collected from Yellow and Bohai Seas was determined by next-generation sequencing. The mitogenome is a circular molecule 16,513 bp in length, including the typical structure of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and a control region. The TAS, central CSB, and CSB were detected in the control region.

Most invasive species largely conserve their climatic niche.

The ecological niche is a key concept for elucidating patterns of species distributions and developing strategies for conserving biodiversity. However, recent times are seeing a widespread debate whether species niches are conserved across space and time (niche conservatism hypothesis). Biological invasions represent a unique opportunity to test this hypothesis in a short time frame at the global scale.

Species distribution models have limited spatial transferability for invasive species.

The reliability of transferring species distribution models (SDMs) to new ranges and future climates has been widely debated. Biological invasions offer the unique opportunity to evaluate model transferability, as distribution data between species' native and introduced ranges are geographically independent of each other. Here, we performed the first global quantitative synthesis of the spatial transferability of SDMs for 235 invasive species and assessed the association of model transferability with the focal invader, model choice and parameterisation.

Seasonal variations of the ichthyoplankton assemblage in the Yangtze Estuary and its relationship with environmental factors.

Seasonal variations of the ichthyoplankton assemblage and its relationship with the environment were analyzed based on four seasonal surveys during 2012. Historical data was collected to be compared with results from previous years in order to indicate the seasonal and inter-annual variation of the ichthyoplankton assemblage in the Yangtze Estuary and the adjacent waters. A total of 3,688 individuals belonging to 5 orders, 9 families, and 15 species were collected.

YY1 binds to the E3' enhancer and inhibits the expression of the immunoglobulin κ gene via epigenetic modifications.

The rearrangement and expression of immunoglobulin genes are regulated by enhancers and their binding transcriptional factors that activate or suppress the activities of the enhancers. The immunoglobulin κ (Igκ) gene locus has three important enhancers: the intrinsic enhancer (Ei), 3' enhancer (E3'), and distal enhancer (Ed). Ei and E3' are both required for Igκ gene rearrangement during early stages of B-cell development, whereas optimal expression of the rearranged Igκ gene relies on both E3' and Ed.

Variations in early life history traits of Japanese anchovy in the Yangtze River Estuary.

Resources of Japanese anchovy ( Temminck & Schlegel, 1846) are undergoing dramatic recessions in China as the consequence of intensifying anthropogenic activities. Elucidating the influences of local-scale environmental factors on early life history traits is of great importance to design strategies conserving and restoring the declining anchovy resources. In this research, we studied hatching date and early growth of anchovy in the Yangtze River Estuary (YRE) using information obtained from otolith microstructure.

The complete mitochondrial genome of the larval Bombay duck (Aulopiformes, Synodontidae) from Yangtze estuary and the phylogenetic relationship of Synodontidae species.

The complete mitochondrial genome of the larvae collected from Yangtze estuary was determined by next-generation sequencing. The mitogenome is a circular molecule 16,536 bp in length, including the typical structure of 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a control region. The TAS, central CSB and CSB were detected in the control region.

Complete mitochondrial genome of the larval (Gasterosteiformes, Syngnathidae) from Yangtze estuary and the phylogenetic relationship of genus .

The complete mitochondrial genome of the larval collected from Yangtze estuary was determined by next-generation sequencing. The mitogenome is a circular molecule 16,465 bp in length, including 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a control region. The TAS, central CSB and CSB were detected in the control region.

Comparative study the expression of calcium cycling genes in Bombay duck () and beltfish () with different swimming activities.

The contraction and relaxation events of the muscle is mediated by the coordination of many important calcium cycling proteins of ryanodine receptor (RYR), troponin C (TNNC), parvalbumin (PVALB), sarcoendoplasmic reticulum calcium transport ATPase (SERCA) and calsequestrin (CASQ). In higher vertebrates, the expression level of calcium cycling proteins are positively correlated to the muscle contraction/relaxation ability of the cell. In this study, we used RNAseq to explore the expression profile of calcium cycling genes between two marine fish of Bombay duck () and beltfish () with poor and robust swimming activities, respectively.

Identification of myogenic regulatory genes in the muscle transcriptome of beltfish (Trichiurus lepturus): A major commercial marine fish species with robust swimming ability.

The beltfish (Trichiurus lepturus) is considered as one of the most economically important marine fish in East Asia. It is a top predator with a robust swimming ability that is a good model to study muscle physiology in fish. In the present study, we used Illumina sequencing technology (NextSeq500) to sequence, assemble and annotate the muscle transcriptome of juvenile beltfish.

Autumn ichthyoplankton assemblage in the Yangtze Estuary shaped by environmental factors.

This study investigated the response of the ichthyoplankton community to environmental changes in the Yangtze Estuary using canonical correspondence analysis. Ichthyoplankton community and environmental data were recorded during the autumns of 1998, 2000, 2002, 2003, 2004, 2007 and 2009. Among the ichthyoplankton, the dominant larval and juvenile families were the Engraulidae, Gobiidae and Salangidae, and the most common eggs were from Trichiurus lepturus.