Cytokine screening identifies TNF to potentially enhance immunogenicity of pediatric sarcomas.

Introduction: Pediatric sarcomas, including osteosarcoma (OS), Ewing sarcoma (EwS) and rhabdomyosarcoma (RMS) carry low somatic mutational burden and low MHC-I expression, posing a challenge for T cell therapies. Our previous study showed that mediators of monocyte maturation sensitized the EwS cell line A673 to lysis by HLA-A*02:01/CHM1-specific allorestricted T cell receptor (TCR) transgenic CD8 T cells (CHM1 CD8 T cells).

Methods: In this study, we tested a panel of monocyte maturation cytokines for their ability to upregulate immunogenic cell surface markers on OS, EwS and RMS cell lines, using flow cytometry.

The "specific" P-glycoprotein inhibitor zosuquidar (LY335979) also weakly inhibits human organic cation transporters.

Zosuquidar (LY335979) is a widely used experimental P-glycoprotein (P-gp) inhibitor, which is commended as very potent but also as very specific for P-gp. In this in vitro and in silico study, we demonstrated for the first time that zosuquidar also inhibits organic cation transporters (OCT) 1-3, albeit less potently than P-gp. This still has to be kept in mind when zosuquidar is used to inhibit cellular efflux of P-gp substrates that are concurrently transported into the cells by OCTs.

A computational framework to optimize the mechanical behavior of synthetic vascular grafts.

The failure of synthetic small-diameter vascular grafts has been attributed to a mismatch in the compliance between the graft and native artery, driving mechanisms that promote thrombosis and neointimal hyperplasia. Additionally, the buckling of grafts results in large deformations that can lead to device failure. Although design features can be added to lessen the buckling potential (e.

Indication and Technical Consideration for Nerve Blocks and Neurolysis for Pain Control.

Pain management in interventional radiology (IR) encompasses a variety of advanced image-guided techniques to deliver minimally invasive treatments for various pain conditions. Key procedures include nerve blocks and neurolysis, which target specific nerves to provide substantial pain relief. Effective pain management in IR relies on a thorough understanding of regional anatomy, precise technique, and careful administration of local anesthetics, neurolytic agents, and corticosteroids.

An extended PFAS profiling of a Swedish subpopulation and mixture risk assessments using multiple approaches.

Per- and polyfluoroalkyl substances (PFAS) have been detected worldwide, from the deep seas to polar regions. A previous review showed that PFAS are risk drivers of the chemical mixture present in human blood. This study focused on establishing the PFAS exposure of a Swedish subpopulation and investigated whether the exposure poses a risk of adverse health effects.

Genetic/Familial High-Risk Assessment: Colorectal, Endometrial, and Gastric, Version 3.2024, NCCN Clinical Practice Guidelines In Oncology.

Multigene panel testing has allowed for the detection of a growing number of inherited pathogenic/likely pathogenic variants in people at high risk of cancer, including endometrial cancer (EC). Hereditary syndromes associated with EC include Lynch syndrome, PTEN hamartoma tumor syndrome, and Peutz-Jeghers syndrome. This manuscript provides the latest recommendations from the NCCN Guidelines for Genetic/Familial High-Risk Assessment: Colorectal, Endometrial, and Gastric on the screening and management of EC in patients at high risk for these syndromes, as well as the advantages and limitations of multigene panel testing.

Early resistance rehabilitation improves functional regeneration following segmental bone defect injury.

Many studies have explored different loading and rehabilitation strategies, yet rehabilitation intensity and its impact on the local strain environment and bone healing have largely not been investigated. This study combined implantable strain sensors and subject-specific finite element models in a 2 mm rodent segmental bone defect model. After injury animals were underwent high or low intensity rehabilitation.

The GATA-3-dependent transcriptome and tumor microenvironment are regulated by eIF4E and XPO1 in T-cell lymphomas.

The transcription factor GATA-3 and the transcriptional program it regulates have emerged as oncogenic drivers across diverse T-cell lymphomas (TCL), many of which are resistant to conventional chemotherapeutic agents and characterized by recurrent losses of key tumor suppressor genes, including TP53 and PTEN, both of which are clients of the nuclear export protein XPO1. Here we demonstrated that XPO1 is highly expressed by malignant T cells expressing GATA-3 and by lymphoma-associated macrophages (LAM) within their tumor microenvironment (TME). Using complementary genetically engineered mouse (GEM) models, we demonstrated that TP53 and/or PTEN deficient TCL, and LAM within their TME, are sensitive to the selective XPO1 antagonist selinexor.

Association between precipitation events, drought, and animal operations with infections in the Southwest US, 2009-2021.

Background: Temperature and precipitation have previously been associated with infections. The association between salmonellosis and precipitation might be explained by antecedent drought conditions; however, few studies have explored this effect.

Methods: Using an ecological study design with public health surveillance, meteorological (total precipitation [inches], temperature [average °F], Palmer Drought Severity Index [PDSI, category]), and livestock data we explored the association between precipitation and infections reported in 127/141 counties from 2009 to 2021 in the Southwest, US and determined how this association was modified by antecedent drought.

Evoking stable and precise tactile sensations via multi-electrode intracortical microstimulation of the somatosensory cortex.

Tactile feedback from brain-controlled bionic hands can be partially restored via intracortical microstimulation (ICMS) of the primary somatosensory cortex. In ICMS, the location of percepts depends on the electrode's location and the percept intensity depends on the stimulation frequency and amplitude. Sensors on a bionic hand can thus be linked to somatotopically appropriate electrodes, and the contact force of each sensor can be used to determine the amplitude of a stimulus.

Developing an instrument to measure the quality of social work cancer counseling regarding return to work - psychometric properties of the German Quality of Cancer Counseling Questionnaire-Return to Work (QCCQ-W).

Purpose: Counseling by social workers can be of great relevance supporting cancer survivors with their psychosocial challenges such as returning to work. However, an instrument for assessing the quality between social worker and client in the cancer counseling setting regarding return to work is not yet available. This study was carried out to develop and validate an instrument for this purpose.

Uncovering the light absorption mechanism of the blue natural colorant allophycocyanin from Arthrospira platensis using molecular dynamics.

Phycobiliproteins of the cyanobacterium Arthrospira platensis, known as Spirulina, are protein-chromophore complexes which are used by the organism to capture light energy. Allophycocyanin and C-phycocyanin are prominent in providing a natural source of blue food coloring. An unresolved issue remains the rapid loss of the native conformation of the pigment, leading to altered color with changing pH.

Effect of Parametric Variation of Chordae Tendineae Structure on Simulated Atrioventricular Valve Closure.

Purpose: Many approaches have been used to model chordae tendineae geometries in finite element simulations of atrioventricular heart valves. Unfortunately, current "functional" chordae tendineae geometries lack fidelity (e.g.

Leveraging Deep Learning of Chest Radiograph Images to Identify Individuals at High Risk for Chronic Obstructive Pulmonary Disease.

Background: This study assessed whether deep learning applied to routine outpatient chest X-rays (CXRs) can identify individuals at high risk for incident chronic obstructive pulmonary disease (COPD).

Methods: Using cancer screening trial data, we previously developed a convolutional neural network (CXR-Lung-Risk) to predict lung-related mortality from a CXR image. In this study, we externally validated CXR-Lung-Risk to predict incident COPD from routine CXRs.

The Nonlinear and Distinct Responses of Ocean Heat Content and Anthropogenic Carbon to Ice Sheet Freshwater Discharge in a Warming Climate.

Anthropogenic climate change will drive extensive mass loss across both the Antarctic (AIS) and Greenland Ice Sheets (GrIS), with the potential for global climate system feedbacks, especially in polar regions. Historically, the high-latitude North Atlantic and Southern Ocean have been critical regions for anthropogenic heat and carbon uptake, but our understanding of how this uptake will be altered by future freshwater discharge is incomplete. We assess each ice sheet's impact on global ocean anthropogenic heat and carbon storage for a high-emission scenario over the -century using a coupled Earth system model.

A Simple Clinical Predictive Model for Arthroscopic Mobility of Osteochondritis Dissecans Lesions of the Knee.

Background: Osteochondritis dissecans (OCD) of the knee is a focal idiopathic alteration of subchondral bone and/or its precursor with risk for instability and disruption of adjacent cartilage. Treatment options focused on preventing premature osteoarthritis vary depending on multiple patient and lesion characteristics, including lesion mobility.

Purpose: To differentiate lesion mobility before arthroscopy using a multivariable model that includes patient demographic characteristics and physical examination findings.

Dynamic light scattering of the eye in the diagnosis of Alzheimer's disease.

The early detection of Alzheimer's disease, before symptoms have appeared, is integral to the development of effective treatments. Dynamic light scattering spectroscopy measures the Brownian movement of proteins at the molecular level. This technique may facilitate early Alzheimer's disease diagnosis and the discovery of pharmaceuticals that may prevent symptom development.

Leptin augments IL-13-induced airway eotaxins and submucosal eosinophilia in obesity-associated asthma.

Background: Airway tissue eosinophilia can be an observed feature of obesity-associated type 2 (T2) asthma, but the processes mediating this inflammation are unknown.

Objective: To investigate a process whereby leptin, an adipokine elevated in obesity, potentiates pulmonary eosinophilia and eotaxin production by airway fibroblasts in T2 asthma.

Methods: We assessed associations between body mass index and airway eosinophilia as well as leptin and eotaxin production in 78 participants with asthma, 36 of whom exhibited obesity.

Repeated use of hospital-based services and delayed hospital discharges in a population-based cohort of autistic adults in Canada.

We know that autistic people have more health problems and are more likely to go to the emergency department and get hospitalized than other people, but we know less about the problems they have once they get to the hospital. In this study, we looked at all autistic adults in Ontario and compared them to adults who were not autistic and to adults who had other kinds of developmental disabilities to see who came back to the emergency department in the month after an emergency department visit, who got re-hospitalized in the month after being sent home from hospital, and who stayed in the hospital longer than they needed to because there was no place appropriate for them to go to. We found that both autistic males and females were more likely to have these things happen to them than the same age- and sex-matched adults who did not have developmental disabilities.

Soybean genomics research community strategic plan: A vision for 2024-2028.

This strategic plan summarizes the major accomplishments achieved in the last quinquennial by the soybean [Glycine max (L.) Merr.] genetics and genomics research community and outlines key priorities for the next 5 years (2024-2028).

Phase I study of allogeneic monocyte-derived inflammatory dendritic cells in combination with pembrolizumab.

PD-1 checkpoint inhibition has revolutionized the care of cancer. A small portion of patients with stage IV cancer achieve durable control. But, early progression is common and dramatic control is achieved for only a minority.

Barriers to Colonoscopy Quality Measurement in Rural Wisconsin.

Background: Patients in rural areas have reduced colonoscopy access, which is critical for colorectal cancer prevention. General surgeons perform most colonoscopies in rural areas. The Surgical Collaborative of Wisconsin's Rural Task Force identified colonoscopy as a high priority initiative due to high volume and lack of quality measure access, both necessary for assessing and improving performance.