ALAD-YOLO:an lightweight and accurate detector for apple leaf diseases.

Suffering from various apple leaf diseases, timely preventive measures are necessary to take. Currently, manual disease discrimination has high workloads, while automated disease detection algorithms face the trade-off between detection accuracy and speed. Therefore, an accurate and lightweight model for apple leaf disease detection based on YOLO-V5s (ALAD-YOLO) is proposed in this paper.

Recombinant Human Decorin Inhibits TGF-b1 Induced Contraction of Collagen Lattice by Keloid Fibroblasts.

While wound contraction plays an important role in healing, it may lead to excessive scar formation and pathological wound contracture in extreme conditions. To date, the key regulator of wound contraction and keloid formation is transforming growth factor-beta (TGF-b1). Decorin has been reported to bind TGF-b1 and neutralize some of its activities.

Recombinant human decorin inhibits TGF-beta1-induced contraction of collagen lattice by hypertrophic scar fibroblasts.

Decorin was reported to bind transforming growth factor-beta (TGF-beta(1)) and neutralise some of its activity as a key regulator of wound contraction and hypertrophic scar formation. In this study, we investigated whether recombinant human decorin affected TGF-beta(1)-induced fibroblast contractile activity, by using fibroblast-populated collagen lattice with decorin added to the collagen gel. Hypertrophic scar fibroblasts showed greater basal contraction of collagen gels than normal fibroblasts, and the addition of TGF-beta(1) significantly enhanced this.

[Bacterial ecology on burn wound and antibacterial agent therapy].

The main factors influencing the bacterial ecology on burn wound are the selection of antibacterial agents and systemic antibiotic. Some antibacterial agents more active against P. aeruginosa were developed in 1960s, and the detection rate of P.

[Protective effect of lytic cocktail (inhibition of stress) on lung injury in severe burn rat].

Objective: To investigate the protective effect of inhibition of stress (lytic cocktail) on lung injury in severe burn rats at early stage.

Methods: Sprague-Dawley rats inflicted with 30% TBSA full-thickness burn were randomly divided into A group (n = 36, fluid resuscitation with administration of lytic cocktail), B group (n = 36, fluid resuscitation only). Lung function was evaluated by partial pressure of oxygen (PaO2) in arterial blood and histopathologic changes on 3, 5, 7, 10 post burn day (PBD).

[An understanding of burn infection].

Burn infection occurs when pathogenic bacteria colonized on the burn wound surface, and they then invaded the viable tissue causing sepsis or sepsis with blood stream invasion. This infection pattern is particular to burn injury. Both in a model of pseudomonas burn wound sepsis and a clinical study of early eschar excision for bacteria quantification indicate that the bacteria not only are located on the burn wound surface but also invaded the deeper tissues.

Recombinant human decorin inhibits cell proliferation and downregulates TGF-beta1 production in hypertrophic scar fibroblasts.

Hypertrophic scarring remains a major problem for patients who have suffered deep burns. The pathophysiology underlying hypertrophic scar formation may be driven by the biological activity of transforming growth factor beta1 (TGF-beta(1)). Decorin is a human proteoglycan that inactivates the effect of TGF-beta(1) and therefore displays a beneficial effect of antifibrosis in various tissues.

[The antagonistic effect of recombinant human decorin on TGF-beta1 stimulation of fibroblasts in collagen lattices].

Objective: To mimic contact pattern between decorin and TGF-beta1, in vivo, and investigate the antagonistic effect of recombinant human decorin on TGF-beta1 stimulation of hypertrophic scar fibroblasts in collagen lattices.

Methods: Fibroblasts populated collagen lattices (FPCL) model was adopted in the study, and they were divided into control group, decorin group [2mg/L recombinant human decorin (rh-decorin) was administered to FPCL], TGF-beta1 group (5 microg/LTGF-beta1 was administered to the culture medium), and TGF-beta1 + decorin group (2mg/L rh-decorin was administered to FPCL, then culture medium containing 5 microg/L TGF-beta1 was added into FPCL). Changes in PAI-1 and alpha-SMA protein expression in scar fibroblasts in collagen lattices were detected with Western blotting at 12 post-administration hour (PAH), 24 PAH, 48 PAH, and 72 PAH, and expressions of PAI-1 and alpha-SMA mRNA were concomitantly examined by RT-PCR.

[The influence of topical application of insulin on the formation of basement membrane in scalded rats].

Objective: To investigate the influence of topical application of insulin on the formation of basement membrane (BM) in rats with deep partial thickness scald.

Methods: Ninety-six SD rats were inflicted with deep partial thickness scald on the back and were randomized into A group (n = 48, with subcutaneous injection of 2 ml of isotonic saline on the 1 post scald day (PSD)) and B group (n = 48, with subcutaneous injection of 0.1 U of insulin and 2 ml of isotonic saline on the 1 PSD).

[Expression of keratinocyte basement membrane related genes during the process of re-epithelialization of burn wound in scalded rats].

Objective: To study the expression of keratinocyte (KC) basement membrane (BM) related genes during the process of re-epithelialization of burn wound in scalded rats with cDNA microarray technique.

Methods: Twenty-four SD rats were inflicted with deep partial thickness scald with an area of 45 cm(2) on the back, and they were randomly divided into A [3 postscald day (PSD)], B (10 PSD), C (14 PSD) and D (re-epithelialization complete day) groups, with 6 rats in each group. Tissue samples were harvested from 1 cm of wound margin on 3, 10 and 14 PSD.

[Influence of stress inhibition on invasive infection in burn wound during early postburn stage in severely burned rats].

Objective: To investigate the influence of inhibition of stress on the survival rate, organ dysfunction and (Th)1/Th2 cytokine profiles of the rats with invasive infection in the wound at early postburn stage.

Methods: Sprague-Dawley rats inflicted with 30% TBSA full thickness burn were randomly divided into A (n = 36, with immediate resuscitation), B (n = 36, with immediate resuscitation and lytic cocktail administration). After subeschar injection of 0.

[Effects of topical application of insulin on the wound healing in scalded rats].

Objective: To investigate the effects of topical application of small dose of insulin on the wound healing of the scalded rats, so as to explore its mechanism.

Methods: The rats employed in the study were subjected to deep partial thickness burn and were divided into group A (with subcutaneous injection of isotonic saline into the rat wounds as control), B and C (with subcutaneous injection of 0.1 U and 1 U insulin in the rat wounds respectively) and D (with subcutaneous injection of 0.

[The content of decorin and its mRNA expression in normal human skin and hyperplastic scars].

Objective: To investigate the content of decorin and its mRNA expression in normal human skin and hyperplastic scars at different stages, so as to explore the relationship between the change of decorin and its synthesis.

Methods: Scar tissue samples from 22 patients undergoing scar excision and 10 specimens of normal skin or prepuce were obtained. The content and distribution of decorin in the tissue samples were determined with immunohistochemistry and Western blot, and the expression of decorin mRNA was detected by in situ hybridization.

[Initiation of systemic administration of antibiotics in treatment of invasive infection of burns].

Objective: To optimize the systemic antibiotics in the treatment of the invasive infection of burns based on the successive monitoring of bacterial species harvested from the burn wound and subeschar tissues and their resistance to antibiotics.

Methods: The data of bacteriological monitoring of burn wound and subeschar tissues in our burn center from 1995 to 2000 were retrospectively analyzed, and the characteristics of distribution and resistance to antibiotics of 1 109 strains from the wound in 612 patients and subeschar tissues in 146 patients were compared statistically by dividing them into two periods, i.e.

[An observation of the basement membrane remodeling after the combined grafting of xenogenic acellular dermal matrix with autoskin in rats].

Objective: To observe the dynamic process of basement membrane remodeling after the combined grafting of xenogenic acellular dermal matrix with autoskin.

Methods: The rat skin wounds were covered with xenogenic porcine acellular dermal matrix overlaid with razor thin autoskin. The skin samples were collected at 1, 2, 3, 4, 8, 12 and 16 post-grafting weeks.

[A multicenter clinical trial of piperacillin/tazobactam in burn infection].

Objective: To evaluate the efficacy of piperacillin/tazobactam in the management of burn infection.

Methods: Sixty-three burn patients were enrolled in the study with burn sepsis or burn area more than 50%TBSA or full skin loss more than 30% TBSA. The administration regime of the antibiotics was 4.

[An experimental study on the influence of inhibition of postburn stress on inflammatory reaction in severely scalded rats].

Objective: To investigate the influence of hibernation drugs on postburn stress and inflammatory reaction in severely scalded rats.

Methods: Sprague-Dawley rats inflicted with 30% TBSA deep partial thickness scalding were employed as the model. The rats were divided into A (scalding with immediate resuscitation), B (scalding with immediate resuscitation and lytic cocktail), C (scalding with delayed resuscitation), D (scalding with delayed resuscitation and lytic cocktail) and E (sham injury) groups.

[Experimental study on the inflammatory and immune responses of xenogenic acellular dermal matrix transplantation combined with thin split-thickness skin autograft].

Objective: To investigate the dynamic process of the inflammatory response and the profile of Th1/Th2 cytokines after xenogenic acellular dermal matrix (ADM) transplantation with thin split-thickness skin autograft overlay.

Methods: SD rats were used in the study. In the control group, thin split-thickness skin autograft (STSG) was transplanted in the full-thickness skin defect of the SD rats; in the experimental group, the xenogenic acellular dermal matrix combined with thin split-thickness skin autograft was transplanted.