Characterization of the microbial communities and their correlations with volatile flavor compounds and physicochemical factors in Bashang suancai, a traditional Chinese pickle.

Bashang suancai is one of the most wellknown traditional fermented vegetables in North China. The study examined the variations in bacterial diversity, physicochemical properties, and volatile flavor compounds (VFCs) of Bashang suancai over a 7-day fermentation period, utilizing Illumina NovaSeq sequencing and headspace solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS). The leading bacterial phyla were , , and , while the predominant bacterial species included , , , , and .

Tailoring Charge Flow in Carbon-Defective Cu-MOF with Pd Nanoparticles: A Boost for Visible Light Organic Photoelectrochemical Transistor in Bioanalysis.

The photoactive material was of significant importance in organic photoelectrochemical transistor (OPECT) bioanalysis as it influences the photoinduced voltage and the μ* product, resulting in a varying sensor sensitivity. The utilization of metal-organic frameworks (MOFs) as photoactive materials in OPECT analysis is promising, yet it remains a grand challenge due to the inherently narrow light absorption range and high electron-hole recombination rate. Herein, Pd NPs were encapsulated as electron acceptors into the Cu-MOF using a double-solvent method, followed by pyrolysis at the proper temperature.

Development of a highly sensitive colloidal gold semiquantitative method for the determination of difenoconazole residues in citrus.

Introduction: Difenoconazole (DIFE) is a common pesticide used in citrus cultivation; excessive intake can cause neurological damage to the organism, and the existing colloidal gold immunochromatographic test strips cannot meet the requirements for the detection of citrus samples.

Methods: Difenoconazole test strip was prepared based on the colloidal gold immunochromatographic technique (GICT), and its application in citrus samples was investigated; with colloidal gold (CG) as the probe, the optimization of GICT parameters, and the determination of reaction method, the immunochromatographic test strips for the detection of DIFE in citrus was developed, and the limit of detection (LOD), specificity, accuracy, and stability of the test strips were verified.

Results: The results showed that the visual detection limit of the prepared colloidal gold immunochromatographic test strips was 0.

In situ study of structural changes: Exploring the mechanism of protein corona transition from soft to hard.

Hypothesis: The process of protein corona changes has been widely believed to follow the Vroman effect, while protein structural change during the process is rarely reported, due to the lack of analytical methods. In-situ interpretation for protein structural change is critical to processes such as the recognition and transport of nanomaterials.

Experiments: Molecular dynamics (MD) simulation was used to predict the deflection and twist of the protein tertiary structure.

Ce/Zr-MOF with Dual Cycle Synergistic Catalysis Pathway Enabling Enhanced Peroxidase-like Performance for Wearable Hydrogel Patch Visualization Sensing Platform.

Engineering the activity of enzyme-like catalysts should be a top priority to make them superior substitutes for natural enzymes. Herein, a Ce/Zr bimetal-organic framework (Ce/Zr-MOF) was designed and synthesized by a one-pot hydrothermal method, which has enhanced performance in mimicking peroxidase (POD) than its single-metal counterparts. To further comprehend the mechanism of activity enhancement, the role of the bimetallic synergistic catalysis process in HO decomposition and reactive oxygen species formation was elucidated, and the possible dual cycle synergistic catalysis pathway of bimetallic catalysis is proposed for the first time.

Isothermal nucleic acid amplification combined with gold nanoparticles assisted electrochemical impedance for the sensitive and efficient porcine delta coronavirus detection.

The emergence of porcine delta coronavirus (PDCoV) has caused huge economic losses in the global pig industry. How to realize the sensitive and efficient detection for it is a difficult problem that need to be resolved. In this work, loop-mediated isothermal amplification (LAMP)-electrochemical impedance spectroscopy (EIS) detection platform for PDCoV based on nucleic acid level was constructed by combining the advantages of efficient amplification for LAMP and sensitive detection for EIS.

Coordination Construction of the Dual Superoxide Dismutase Catalytic Center Nanozyme: Synergistic Contribution of the Site, Structure, and Electron Transfer Pathway.

Superoxide dismutase (SOD) mimics are limited by a single active center, and their performance is difficult to achieve the activity level of natural SOD. Herein, we exhibit the coordination construction of different SOD active centers (Cu and Mn) and structural regulation of framework carbonization in MOFs. The obtained catalytic activity and excellent biocompatibility are comparable to Cu/Zn-SOD.

Soft integration of a neural cells network and bionic interfaces.

Both glial cells and neurons can be considered basic computational units in neural networks, and the brain-computer interface (BCI) can play a role in awakening the latency portion and being sensitive to positive feedback through learning. However, high-quality information gained from BCI requires invasive approaches such as microelectrodes implanted under the endocranium. As a hard foreign object in the aqueous microenvironment, the soft cerebral cortex's chronic inflammation state and scar tissue appear subsequently.

Simulation design of a binding-pocket structure of natural enzymes in MOFs for enhanced catalytic activity.

We demonstrated that the activity gap between metal-organic frameworks (Fe) and horseradish peroxidase could be bridged by simulating the binding-pocket structure and adding active centers. This customized structure promoted the activation and enrichment of substrates, and addition of gold nanoparticles led to activity superposition and synergistic enhancement.

Hierarchical Regulation of LaMnO Dual-Pathway Strategy for Excellent Room-Temperature Organocatalytic Oxidation Performance.

The performance-enhancing strategy of a single pathway for perovskite has been widely studied. In this work, the dual-pathway strategy of A-site Ce substitution and nitric acid selective dissolution was proposed. The catalytic oxidation performance of LaMnO exhibits the characteristic of hierarchical regulation, that is, a steplike improvement, which avoids the limitation of performance improvement of the single pathway.

Ultrasensitive photoelectrochemical aptasensor for carbendazim detection based on in-situ constructing Schottky junction via photoreducing Pd nanoparticles onto CdS microsphere.

Carbendazim (CBZ) has been widely used in agricultural production to control fruits and vegetables diseases, but it can also destroy the human endocrine system. Therefore, sensitive detection of CBZ has attracted increasing attention worldwide. In this study, Pd nanoparticles (Pd NPs) decorated on CdS microsphere (Pd NPs/CdS) was prepared by the in-situ photoreduced method, and based on the surface plasmon resonance (SPR) effect of noble metal and Schottky junction between Pd nanoparticles (Pd NPs) and CdS microsphere, the photocurrent after introducing Pd NPs is 7.

A colorimetric biosensor for simultaneous ochratoxin A and aflatoxins B1 detection in agricultural products.

Colorimetric biosensors have been widely applied to mycotoxins testing. However, the colorimetric assay previously reported used a single color to detect one mycotoxin, and there were few reports on the simultaneous detection of multiple mycotoxins. In this work, a colorimetric biosensor for dual mycotoxins detection was developed.

Highly active metal-free peroxidase mimics based on oxygen-doped carbon nitride by promoting electron transfer capacity.

A heteroatom oxygen doping strategy was developed to prepare oxygen-doped carbon nitride (OCN). The performance and mechanism of its peroxidase-like activity were studied for the first time. The greatly improved peroxidase-like activity could be attributed to the promoted electron transferability.

Oxygen Vacancy Engineering in Europia Clusters/Graphite-Like Carbon Nitride Nanostructures Induced Signal Amplification for Highly Efficient Electrochemiluminesce Aptasensing.

Oxygen vacancy is an intrinsic defect in metal oxide semiconductors and has a crucial influence on their physicochemical and electronic properties. To boost the electrochemiluminescence (ECL) efficiency of the graphite-like carbon nitride (g-CN), the wet-chemical-calcination method was developed to introduce an oxygen vacancy in Eu-doped g-CN nanostructures for the first time. The morphology and structure characterization suggest that the Eu element was present in the matrix of the europia (EuO) clusters.