High temperatures increase the virulence of Vibrio bacteria towards their coral host and competing bacteria via type VI secretion systems.

The bacterial pathogen Vibrio coralliilyticus induces severe coral diseases in warming oceans. A study in PLOS Biology reveals that high temperatures activate 2 type VI secretion systems in V. coralliilyticus, enhancing pathogenicity by deploying toxic effectors against competing bacteria and coral cells.

Study on the photoaging process and metal ion release of plastic films with two kinds of structures in marine environment: Aliphatic and aromatic polymers.

The prevalence of plastics in the oceans has significantly intensified microplastic pollution, contributing to broader marine secondary pollution issues. This paper examines how plastic structure affects the aging characteristics of plastics and the release of metal ions, to better understand this secondary source of marine pollution. This study simulate the photoaging of plastics in natural environments, focusing on aliphatic and aromatic polymers.

Combining CRISPR/Cas9 and natural excision for the precise and complete removal of mobile genetic elements in bacteria.

Horizontal gene transfer, facilitated by mobile genetic elements (MGEs), is an adaptive evolutionary process that contributes to the evolution of bacterial populations and infectious diseases. A variety of MGEs not only can integrate into the bacterial genome but also can survive or even replicate like plasmids in the cytoplasm, thus requiring precise and complete removal for studying their strategies in benefiting host cells. Existing methods for MGE removal, such as homologous recombination-based deletion and excisionase-based methods, have limitations in effectively eliminating certain MGEs.

Tumor-infiltrating immune cells and survival in head and neck squamous cell carcinoma: a retrospective computational study.

The immune infiltration profiles of the tumor microenvironment have effects on the prognosis of head and neck squamous cell carcinoma (HNSCC). Whereas, HNSCC is a heterogeneous group of tumors, but past work has not taken this into consideration. Herein, we investigate the associations between survival and the function of immune cells in different tumorigenic sites of HNSCC.

Genomic Island-Encoded Diguanylate Cyclase from Regulates Biofilm Formation and Motility in .

Many bacteria use the second messenger c-di-GMP to regulate exopolysaccharide production, biofilm formation, motility, virulence, and other phenotypes. The c-di-GMP level is controlled by the complex network of diguanylate cyclases (DGCs) and phosphodiesterases (PDEs) that synthesize and degrade c-di-GMP. In addition to chromosomally encoded DGCs, increasing numbers of DGCs were found to be located on mobile genetic elements.

Biodegradable cellulose/curcumin films with Janus structure for food packaging and freshness monitoring.

The development of renewable, hydrophobic, and biodegradable intelligent packaging materials as an alternative to petroleum-based plastic products has become a new research focus in recent years, but remains a challenge. Herein, regenerated cellulose/curcumin composite films were fabricated by dispersing hydrophobic curcumin uniformly in a hydrophilic cellulose matrix using an aqueous alkali/urea solvent based on the pH-driven principle of curcumin. In addition, a unilateral hydrophobic modification was carried out using chemical vapor deposition of methyltrichlorosilane to obtain Janus structure.

Gut microbial structural variation associates with immune checkpoint inhibitor response.

The gut microbiota may have an effect on the therapeutic resistance and toxicity of immune checkpoint inhibitors (ICIs). However, the associations between the highly variable genomes of gut bacteria and the effectiveness of ICIs remain unclear, despite the fact that merely a few gene mutations between similar bacterial strains may cause significant phenotypic variations. Here, using datasets from the gut microbiome of 996 patients from seven clinical trials, we systematically identify microbial genomic structural variants (SVs) using SGV-Finder.

The Secondary Metabolites of Bacillus subtilis Strain Z15 Induce Apoptosis in Hepatocellular Carcinoma Cells.

The lipopeptides produced by Bacillus subtilis have anti-cancer potential. We had previously identified a secondary metabolite of B. subtilis strain Z15 (BS-Z15), which has an operon that regulates lipopeptide synthesis, and also demonstrated that the fermentation products of this strain exerted antioxidant and pro-immune effects.

Molecular basis of the phenotypic variants arising from a mutator.

Bacterial deficiencies in the DNA repair system can produce mutator strains that promote adaptive microevolution. However, the role of mutator strains in marine , capable of generating various gain-of-function genetic variants within biofilms, remains largely unknown. In this study, inactivation of in conferred an approximately 100-fold increased resistance to various antibiotics, including ciprofloxacin, rifampicin and aminoglycoside.

Microscopic dissolution process of cellulose in alkaline aqueous solvents and its application in CNFs extraction - Investigating temperature as a variable.

The target of this study is to gain a deeper understanding of the micro-dissolution process of cellulose in alkaline aqueous solutions and to develop a novel method for extracting cellulose nanofibrils (CNFs). Herein, the dissolution process of cellulose in alkaline aqueous solutions will be controlled by varying the temperature, and the undissolved cellulose will be analyzed to reveal the microscopic dissolution process of cellulose, and a novel process for extracting cellulose nanofibrils (CNFs) will be developed based on the findings. The crystalline structure of cellulose was gradually disrupted as the dissolution progressed, and the crystal form of cellulose changed gradually from cellulose I to cellulose II during the dissolution process, while all undissolved cellulose crystals remained as cellulose I.

Comparative genomic insights into habitat adaptation of coral-associated .

Green sulfur bacteria (GSB) are a distinct group of anoxygenic phototrophic bacteria that are found in many ecological niches. , a marine representative genus of GSB, was found to be dominant in some coral skeletons. However, how coral-associated (CAP) adapts to diurnal changing microenvironments in coral skeletons is still poorly understood.

Multicolor biosensor for hyaluronidase based on target-responsive hydrogel and etching of gold nanorods by HO.

Hyaluronidase (HAase) is a potential tumor biomarker for diseases of the digestive tract and nervous system, the development of simple and sensitive techniques for HAase determination is urgent needed. Gold nanorods (Au NRs) can be etched by HO with high efficiency and display color changing. In this work, a HAase-responsive hydrogel system had been designed and the amount of HO spilled from the system had a close relationship with the amount of HAase, then the spilled HO had been applied to etch Au NRs.

Working from Home in Urban China during the COVID-19 Pandemic: Assemblages of Work-Family Interference.

During the COVID-19 pandemic, millions of workers globally have been forced to work from home. Empirical data from Chinese cities in the Hubei province reveal work productivity decreased among many respondents working from home in 2020, primarily due to family interference with work. Such interference stems not only from the domain of daily life but also from other family members' e-working and e-learning.

Patterns of immune infiltration and survival in endocrine therapy-treated ER-positive breast cancer: A computational study of 1900 patients.

Tumor-infiltrating immune cells (TIICs) play a critical role in breast cancer (BC) prognosis, but little is known regarding the efficacy of endocrine therapy in patients with ER-positive BC with diverse immunological phenotypes. To investigate whether TIICs affect survival after endocrine therapy in patients with different BC molecular subtypes, data were gathered from six studies totaling 1900 samples. CIBERSORTx was used to analyze the invasion of 22 immune cell subpopulations using a bulk gene expression profile.

The coral pathogen Vibrio coralliilyticus kills non-pathogenic holobiont competitors by triggering prophage induction.

The coral reef microbiome is central to reef health and resilience. Competitive interactions between opportunistic coral pathogens and other commensal microbes affect the health of coral. Despite great advances over the years in sequencing-based microbial profiling of healthy and diseased coral, the molecular mechanism underlying colonization competition has been much less explored.

Mobile genetic elements used by competing coral microbial populations increase genomic plasticity.

Intraspecies diversification and niche adaptation by members of the Vibrio genus, one of the most diverse bacterial genera, is thought to be driven by horizontal gene transfer. However, the intrinsic driving force of Vibrio species diversification is much less explored. Here, by studying two dominant and competing cohabitants of the gastric cavity of corals, we found that a phenotype influencing island (named VPII) in Vibrio alginolyticus was eliminated upon coculturing with Pseudoalteromonas.

The HipAB Toxin-Antitoxin System Stabilizes a Composite Genomic Island in CN-32.

Composite genomic islands (GIs) are useful models for studying GI evolution if they can revert into the previous components. In this study, CGI48-a 48,135-bp native composite GI that carries GI21, whose homologies specifically integrated in the conserved gene-were identified in CN-32. CGI48 was integrated into the tRNA gene, which is a conserved gene locus for the integration of genomic islands in .

Filamentous prophage capsid proteins contribute to superinfection exclusion and phage defence in Pseudomonas aeruginosa.

Filamentous prophages in Pseudomonas aeruginosa PAO1 are converted to superinfective phage virions during biofilm development. Superinfection exclusion is necessary for the development of resistance against superinfective phage virions in host cells. However, the molecular mechanisms underlying the exclusion of superinfective Pf phages are unknown.

Molecular Basis of Wrinkled Variants Isolated From Biofilms.

Many species are dominant biofilm-forming Gammaproteobacteria in the ocean. The formation of biofilms is often accompanied by the occurrence of variants with different colony morphologies that may exhibit increased marine antifouling or anticorrosion activities. However, the genetic basis of the occurrence of these variants remains largely unexplored.

Attribution of Community Emergency Volunteer Behaviour During the COVID-19 Pandemic: A Study of Community Residents in Shanghai, China.

Based on grounded theory, the present study summarizes the transcripts from 32 in-depth interviews with Chinese community emergency volunteers to uncover the attributions of community emergency volunteering in China during the COVID-19 pandemic. Community emergency volunteering in China is affected by four main factors: inner awareness, the external environment, national policy, and publicity and advocacy. Among these factors, inner awareness and the external environment are the internal and social psychological attributions, respectively, of emergency volunteering.

Prophage Tracer: precisely tracing prophages in prokaryotic genomes using overlapping split-read alignment.

The life cycle of temperate phages includes a lysogenic cycle stage when the phage integrates into the host genome and becomes a prophage. However, the identification of prophages that are highly divergent from known phages remains challenging. In this study, by taking advantage of the lysis-lysogeny switch of temperate phages, we designed Prophage Tracer, a tool for recognizing active prophages in prokaryotic genomes using short-read sequencing data, independent of phage gene similarity searching.

The impact of national energy structure on the concentrations, environmental behavior, and sources of polycyclic aromatic hydrocarbons in riverine and coastal sediments of the Beibu Gulf, China.

In this study, polycyclic aromatic hydrocarbons (PAHs) were measured in sediments of the Beibu Gulf in 2017 to investigate sources and a risk assessment. The results showed the total PAH concentration (∑PAHs) in sediments of the Beibu Gulf in 2017 (17.6 ± 16.