Evaluation of a Low-Temperature Immersion Immunization Strategy for the Infectious Spleen and Kidney Necrosis Virus Gene-Deleted Attenuated Vaccine.

Background: Infectious spleen and kidney necrosis virus (ISKNV) poses a significant threat to aquaculture sustainability, particularly affecting mandarin fish () and causing significant economic losses.

Methods: To address this challenge, this study developed an ISKNV Δ vaccine strain, where the gene was knocked out. Infection assays conducted at 28 °C showed that the knocking out the gene decreased the virulence of ISKNV and reduced lethality against mandarin fish by 26.

Low-temperature immunization attenuates the residual virulence of gene-deleted infectious spleen and kidney necrosis virus: a candidate immersion vaccine.

Global aquaculture production yielded a record of 122.9 million tons in 2022. However, ~10% of farmed aquatic animal production is lost each year due to various infectious diseases, resulting in substantial economic waste.

A Safe and Efficient Double-Gene-Deleted Live Attenuated Immersion Vaccine to Prevent the Disease Caused by the Infectious Spleen and Kidney Necrosis Virus.

Infectious diseases seriously threaten sustainable aquaculture development, resulting in more than $10 billion in economic losses annually. Immersion vaccines are emerging as the key technology for aquatic disease prevention and control. Here, a safe and efficacious candidate immersion vaccine strain (Δ) of infectious spleen and kidney necrosis virus (ISKNV), in which the and genes were knocked out by homologous recombination, is described.

Interaction of Teleost Fish TRPV4 with DEAD Box RNA Helicase 1 Regulates Iridovirus Replication.

Viral diseases are a significant risk to the aquaculture industry. Transient receptor potential vanilloid 4 (TRPV4) has been reported to be involved in regulating viral activity in mammals, but its regulatory effect on viruses in teleost fish remains unknown. Here, the role of the TRPV4-DEAD box RNA helicase 1 (DDX1) axis in viral infection was investigated in mandarin fish (Siniperca chuatsi).

Mechanism of Detecting the Construction Quality of a Diaphragm Wall by an Infrared Thermal Field and Engineering Application.

During underground space exploitation in the urbanization process, numerous foundation pits were constructed where a diaphragm wall was often used as a retaining structure and waterproof curtain. Due to complicated engineering geological conditions or improper construction, diaphragm walls and wall joints often exhibit quality defects. Groundwater leaked from these quality defects to foundation pits during excavation, endangering the safety of the pit and surrounding facilities.

The Interaction of Mandarin Fish DDX41 with STING Evokes type I Interferon Responses Inhibiting Ranavirus Replication.

DDX41 is an intracellular DNA sensor that evokes type I interferon (IFN-I) production via the adaptor stimulator of interferon gene (STING), triggering innate immune responses against viral infection. However, the regulatory mechanism of the DDX41-STING pathway in teleost fish remains unclear. The mandarin fish () is a cultured freshwater fish species that is popular in China because of its high market value.

Conceptual Model, Experiment and Numerical Simulation of Diaphragm Wall Leakage Detection Using Distributed Optical Fiber.

Leakage in the diaphragm wall is difficult to detect in deep foundation pits. In this study, the conceptual model of active and passive thermal leak detection methods was proposed according to the occurrence of temperature field anomalies caused by seepage. Experiments were performed using a heating system and an optical fiber temperature measurement system to verify the thermal leakage detection systems.

Numerical simulation of foundation pit dewatering using horizontal seepage reducing body.

Groundwater level has to be lowered during deep excavation. A vertical curtain is usually adopted to control the drawdown inside and outside a foundation pit in a built-up area. However, the cost and working difficulty increases substantially with the rise in depth of vertical curtains.

Development of a gene-deleted live attenuated candidate vaccine against fish virus (ISKNV) with low pathogenicity and high protection.

Aquaculture provides important food, nutrition, and income sources for humans. However, aquaculture industry is seriously threatened by viral diseases. Infectious spleen and kidney necrosis virus (ISKNV) disease causes high mortality and economic losses to the fish culture industry in Asia and has been listed as a certifiable disease by the International Epizootic Office.

Predictions and mitigation strategies of PM concentration in the Yangtze River Delta of China based on a novel nonlinear seasonal grey model.

High delicate particulate matter (PM) concentration can seriously reduce air quality, destroy the environment, and even jeopardize human health. Accordingly, accurate prediction for PM plays a vital role in taking precautions against upcoming air ambient pollution incidents. However, due to the disturbance of seasonal and nonlinear characteristics in the raw series, pronounced forecasts are confronted with tremendous handicaps, even though for seasonal grey prediction models in the preceding researches.

Deletion of the Infectious spleen and kidney necrosis virus ORF069L reduces virulence to mandarin fish Siniperca chuatsi.

Mandarin fish (Siniperca chuatsi) is a significant cultured species with high added value in China. With the expansion of farming, diseases of mandarin fish such as Infectious spleen and kidney necrosis virus (ISKNV) diseases are becoming more and more serious. Human endogenous retrovirus subfamily H long terminal repeat associating protein 2 (HHLA2) is a type 1 transmembrane molecule with three extracellular Ig domains (IgV-IgC-IgV) and plays important roles in the T cell proliferation and tumorigenesis.