Density Measurement and Prediction Model of the CO-Water-Bearing Crude Oil System.

Accurately predicting the density variation trend of water-bearing crude oil in high-CO-concentration production wells is of great significance for forecasting wellbore fluid flow dynamics and designing lifting processes at different stages of development. Indoor experiments were conducted on the CO-water-bearing crude oil system, measuring the crude oil density under various conditions of temperature, pressure, CO concentration, and water cut. The study explored the behavior of crude oil density under different working conditions, and a predictive model for high-CO-concentration water-bearing crude oil density was developed using multiple regression analysis.

A VSV-based oral rabies vaccine was sentineled by Peyer's patches and induced a timely and durable immune response.

The global eradication of canine-mediated human rabies remains an ongoing public health priority. While conventional oral rabies vaccines (ORVs) have demonstrated partial success in interrupting zoonotic transmission, current formulations necessitate improvements in both immunogenic profiles and mechanistic clarity. Herein, we present a recombinant vesicular stomatitis virus (VSV)-vectored vaccine candidate (rVSVΔG-ERA-G) engineered to express the glycoprotein of the rabies virus (RABV) ERA strain, substituting the native VSV glycoprotein.

The associations between evening eating and quality of energy and macronutrients and obesity: the National Health and Nutrition Examination Survey (NHANES), 2003-2016.

Background: This study aimed to investigate the associations between evening eating and quality of energy and macronutrients and obesity among U.S. adults.

Recent Advances in Glutathione Depletion-Enhanced Porphyrin-Based nMOFs for Photodynamic Therapy.

Photodynamic therapy has established itself as a clinical treatment for certain superficial cancers by converting oxygen into cytotoxic singlet oxygen to eradicate cancer cells. Porphyrin-based nanoscale metal-organic frameworks have emerged as promising photosensitive platforms due to their ability to prevent the hydrophobic aggregation quenching of porphyrin molecules and enhance accumulation at the tumor site, thereby becoming a focal point in photodynamic materials research. However, the elevated levels of glutathione and other reductive substances within cancer cells can alleviate the oxidative stress induced by singlet oxygen from the photodynamic therapy process, thus protecting intracellular biomolecular structures from damage.

The effect of baseline versus early glucocorticoid use on immune checkpoint inhibitor efficacy in patients with advanced NSCLC.

Purpose: This study aims to investigate the specific effects of glucocorticoids (GC) on the efficacy of immune checkpoint inhibitors (ICIs), and whether this effect is influenced by the timing and dosage of GC administration. Changes in the neutrophil percentage and the helper/suppressor T lymphocyte ratio [NEUT %/(CD4+/CD8+)] during GC administration were monitored.

Methods: The clinical results of 130 patients with advanced non-small cell lung cancer (NSCLC) treated with ICIs were analyzed and compared with those of patients who did not use GC.

Soil minerals regulate soil organic carbon accumulation through glomalin-related soil protein along an elevation gradient in a mountain arid ecosystem.

Soil minerals and glomalin-related soil protein (GRSP), as key soil binding agents, play a crucial role in enhancing soil organic carbon (SOC) sequestration. However, the key driving mechanisms of SOC accumulation process mediated by soil minerals and GRSP remain poorly understood in the arid elevation gradients. This study aimed to evaluate how soil minerals and GRSP affect SOC accumulation across an elevation gradient ranging from 1707 to 3548 m a.

Influences of roughness and filling degree on the shear strength and damage evolution characteristics of cement-filled joints.

The shear resistance of filling joints is an important factor affecting the stability of rock joints. Pressure-shear tests of cement-filled joints were carried out. Combined with the acoustic emission (AE) technique, the effects of normal stress, roughness and filling degree on the shear strength, damage morphology and damage evolution of cement-filled joints were investigated.

Sensitive and specific LAMP and multiplex qRT-PCR assays for detection of hypervirulent Klebsiella pneumoniae.

Hypervirulent Klebsiella pneumoniae (hvKP) pose significant challenges to clinical anti-infective treatment and has emerged as a major threat to global public health. In this study, we employed the loop-mediated isothermal amplification (LAMP) assays with OTG (orange to green) visual dye and multiplex quantitative real-time PCR (qRT-PCR) assay to rapidly detect hvKP. We determined the detection limits of the LAMP methods for K.

Amplification-free detection of using CRISPR-Cas12a and graphene field-effect transistors.

Current molecular tests for tuberculosis (TB), such as whole genome sequencing and Xpert /rifampicin resistance assay, exhibit limited sensitivity and necessitate the pre-amplification step of target DNA. This limitation greatly increases detection time and poses an increased risk of infection. Here, we present a graphene field-effect transistor (GFET) based on the CRISPR/Cas system for detecting .

Increased Mineral-Associated Organic Carbon and Persistent Molecules in Allochthonous Blue Carbon Ecosystems.

Coastal wetlands contain very large carbon (C) stocks-termed as blue C-and their management has emerged as a promising nature-based solution for climate adaptation and mitigation. The interactions among sources, pools, and molecular compositions of soil organic C (SOC) within blue C ecosystems (BCEs) remain elusive. Here, we explore these interactions along an 18,000 km long coastal line of salt marshes, mangroves, and seagrasses in China.

Recent Advances in Porphyrin-Based Covalent Organic Frameworks for Synergistic Photodynamic and Photothermal Therapy.

Porphyrin's excellent biocompatibility and modifiability make it a widely studied photoactive material. However, its large π-bond conjugated structure leads to aggregation and precipitation in physiological solutions, limiting the biomedical applications of porphyrin-based photoactive materials. It has been demonstrated through research that fabricating porphyrin molecules into nanoscale covalent organic frameworks (COFs) structures can circumvent issues such as poor dispersibility resulting from hydrophobicity, thereby significantly augmenting the photoactivity of porphyrin materials.

[Antibiotic Pollution Level and Ecological Risk Assessment of Township Wastewater Treatment Plants].

Due to the abuse of antibiotics, a large amount of antibiotics has been entering wastewater treatment plants （WWTPs）, but the pollution of antibiotics in township WWTPs has not attracted much attention. To understand the contamination level and removal characteristics, and the risks to aquatic organisms and human health, samples collected from the inlet and outlet of 15 township WWTPs were investigated. The results showed that tetracyclines （TCs） had the highest concentration in the inlet and outlet waters, in which the concentrations of TC and oxytetracycline （OTC） reached （4 943.

The Application of the NOSES System to Treat Cheek Squamous Cell Carcinoma.

Purpose: Oral squamous cell carcinoma is one of the most common types of cancer in the head and neck. Squamous cell carcinoma of the buccal mucosa is an important part of it. The traditional surgical method leads to great injury and is accompanied by unacceptable scars.

Recent advances in cell membrane-coated porphyrin-based nanoscale MOFs for enhanced photodynamic therapy.

Porphyrins-based nanoscale metal-organic frameworks (nMOFs) has been widely utilized to kills tumor cells by generating cytotoxic reactive oxygen species (ROS). However, porphyrin based nMOFs (por-nMOFs) still face challenges such as rapid immune clearance and weak tumor targeting. Researchers have discovered that using a top-down biomimetic strategy, where nMOFs are coated with cell membranes, can promote long blood circulation, evade the reticuloendothelial system, and improve cancer cell targeting, thereby significantly enhancing the photodynamic therapy (PDT) effect of nMOFs.

Synergistic air pollution exposure elevates depression risk: A cohort study.

Depression is a leading mental health disorder worldwide, contributing substantially to the global disease burden. While emerging evidence suggests links between specific air pollutants and depression, the potential interactions among multiple pollutants remain underexplored. Here we show the influence of six common air pollutants on depressive symptoms among middle-aged and older Chinese adults.

Conversion of coastal wetlands to paddy fields substantially decreases methane oxidation potential and methanotrophic abundance on the eastern coast of China.

Coastal wetland ecosystems play a key role in the global carbon cycle and climate mitigation. The land conversion of coastal wetlands to paddy fields, an increasingly common practice to feed the growing population, has been shown to dramatically stimulate the methane emissions of (CH). However, the knowledge about how such wetland conversion affects the methane oxidation, a key process regulating methane emissions from coastal wetlands, is nearly unknown.

Ultra-broadband bismuth-doped fiber amplifier with 170 nm bandwidth using a two-stage configuration.

We present a two-stage bismuth-doped fiber amplifier (BDFA) that achieves ultra-broadband amplification from 1280 to 1480 nm. The two-stage BDFA is designed to limit the reabsorption of bismuth-doped silica fiber (BDSF) by high-power pumps pulled fluorescence. A peak gain of 37.

Inducing high-concentration Tb with free oxygen via atomic layer deposition.

Precise preparation and control of trivalent states in rare earth metal oxide films are crucial for their optical and magnetic applications. In this study, compact and continuous terbium-doped nanofilms were deposited on silica substrates using atomic layer deposition (ALD). The average nanoparticle size varied from 17.

Plant-Derived Exosome-Like Nanovesicles in Chronic Wound Healing.

The incidence of chronic wounds is steadily increasing each year, yet conventional treatments for chronic wounds yield unsatisfactory results. The delayed healing of chronic wounds significantly affects patient quality of life, placing a heavy burden on patients, their families, and the healthcare system. Therefore, there is an urgent need to find new treatment methods for chronic wounds.

Porphyrin-engineered nanoscale metal-organic frameworks: enhancing photodynamic therapy and ferroptosis in oncology.

Photodynamic therapy and ferroptosis induction have risen as vanguard oncological interventions, distinguished by their precision and ability to target vulnerabilities in cancer cells. Photodynamic therapy's non-invasive profile and selective cytotoxicity complement ferroptosis' unique mode of action, which exploits iron-dependent lipid peroxidation, offering a pathway to overcome chemoresistance with lower systemic impact. The synergism between photodynamic therapy and ferroptosis is underscored by the depletion of glutathione and glutathione peroxidase four inhibitions by photodynamic therapy-induced reactive oxygen species, amplifying lipid peroxidation and enhancing ferroptotic cell death.

Iron-bound organic carbon declined after estuarine wetland reclamation into paddy fields.

Iron-bound organic carbon (Fe-OC) is a main pathway for the long-term maintenance of soil organic carbon (SOC), but research on its mechanism is still relatively weak. We investigated the coupling relationships among iron (Fe), carbon (C) and Fe-reducing bacteria (FeRB) in the soil of a reclaimed paddy field in comparison with natural Phragmites australis wetland in the Minjiang River estuary in southeastern China. The results showed that conversion of P.