Towards the completion of Koch's postulates for the citrus huanglongbing bacterium, Liberibacter asiaticus.

Liberibacter asiaticus (Las) is one of the causal agents of huanglongbing (HLB), the most devastating disease of citrus worldwide. Due to the intracellular lifestyle and significant genome reduction, culturing Las has proven to be extremely challenging. In this study, we optimized growth conditions and developed a semi-selective medium based on the results of nutritional and antibiotic screening assays.

Explainable exercise recommendation with knowledge graph.

Recommending suitable exercises and providing the reasons for these recommendations is a highly valuable task, as it can significantly improve students' learning efficiency. Nevertheless, the extensive range of exercise resources and the diverse learning capacities of students present a notable difficulty in recommending exercises. Collaborative filtering approaches frequently have difficulties in recommending suitable exercises, whereas deep learning methods lack explanation, which restricts their practical use.

6-Methyl-5-hepten-2-one promotes programmed cell death during superficial scald development in pear.

Plants possess the ability to induce programmed cell death (PCD) in response to abiotic and biotic stresses; nevertheless, the evidence on PCD initiation during pear scald development and the involvement of the scald trigger 6-methyl-5-hepten-2-one (MHO) in this process is rudimentary. Pyrus bretschneideri Rehd. cv.

PbrWRKY62-PbrADC1 module involves in superficial scald development of Pyrus bretschneideri Rehd.fruit via regulating putrescine biosynthesis.

Putrescine plays a role in superficial scald development during the cold storage of pear fruit. However, the molecular mechanism behind this phenomenon has not been un-fully clarified until recently. In this study, a conjoint analysis of metabolites and gene expression profiles in the putrescine-metabolic pathway of P.

Risk-based regionalization approach for area-wide management of HLB vectors in the Mediterranean Basin.

Huanglongbing (HLB) is one of the most devastating citrus diseases worldwide. It is associated with the non-culture bacteria Liberibacter spp., which can be transmitted by grafting and/or the psyllid vectors (ACP) and (AfCP).

PbrbZIP15 promotes sugar accumulation in pear via activating the transcription of the glucose isomerase gene PbrXylA1.

The composition and abundance of soluble sugars in mature pear (Pyrus) fruit are important for its acceptance by consumers. However, our understanding of the genes responsible for soluble sugar accumulation remains limited. In this study, a S1-group member of bZIP gene family, PbrbZIP15, was characterized from pear genome through the combined analyses of metabolite and transcriptome data followed by experimental validation.

ZnO@BiOI Heterojunction Derived from ZIF-8@BiOI for Enhanced Photocatalytic Activity under Visible Light.

In the study, ZIF-8@BIOI composites were synthesized by the hydrothermal method and then calcined to acquire the ZnO@BiOI composite as a novel composite for the photocatalytic deterioration of the antibiotic tetracycline (TC). The prepared ZnO@BiOI composites were physically and chemically characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), Brunauer-Emmet-Teller (BET) surface area, UV-Vis diffuse reflectance spectroscopy (DRS), emission fluorescence spectra, transient photocurrent response, electrochemical impedance spectra and Mott-Schottky. Among the composites formed an n-n heterojunction, which increased the separation efficiency of electrons and holes and the efficiency of charge transfer.

MOF composites derived BiFeO@BiOI n-n heterojunction for enhanced photocatalytic performance.

BiFeOis a photocatalyst with excellent performance. However, its applications are limited due to its wide bandgap. In this paper, MIL-101(Fe)@BiOI composite material is synthesized by hydrothermal method and then calcined at high temperature to obtain BiFeO@BiOI composite material with high degradation capacity.

Feature Distillation in Deep Attention Network Against Adversarial Examples.

Deep neural networks (DNNs) are easily fooled by adversarial examples. Most existing defense strategies defend against adversarial examples based on full information of whole images. In reality, one possible reason as to why humans are not sensitive to adversarial perturbations is that the human visual mechanism often concentrates on most important regions of images.

Identification of a Chromosomal Deletion Mutation and the Dynamics of Two Major Populations of ' Liberibacter asiaticus' in Its Hosts.

' Liberibacter asiaticus' (Las) is the prominent species of associated with huanglongbing, a devastating disease of citrus worldwide. In this study, we report the identification of an ∼8.3-kb DNA region of the Las genome containing eight putative open reading frames flanked by two inverted repeats, which was not present in the Las str.

Direct Fidelity Estimation of Quantum States Using Machine Learning.

In almost all quantum applications, one of the key steps is to verify that the fidelity of the prepared quantum state meets expectations. In this Letter, we propose a new approach solving this problem using machine-learning techniques. Compared to other fidelity estimation methods, our method is applicable to arbitrary quantum states, the number of required measurement settings is small, and this number does not increase with the size of the system.

Preparation of Room Temperature Vulcanized Silicone Rubber Foam with Excellent Flame Retardancy.

To retard the spread of fire in many cases with sealing materials is significant. A series of silicone rubber foam materials were prepared with room temperature vulcanization and foaming reactions. The morphology, chemical structure, cell structure, and thermal stability were investigated and results proved that the synthesis of silicone rubber was successful in a wide range of feed ratios.

Identification and characterization of invertase family genes reveal their roles in vacuolar sucrose metabolism during Pyrus bretschneideri Rehd. fruit development.

23 invertase (PbrInvs) genes, including eight vacuolar invertases (PbrvacInvs), five cell wall invertases (PbrcwInvs) and 10 alkaline/neutral invertases (PbrA/N-Invs), were identified from P. bretschneideri Rehd. genome, with diverse chromosome locations, cis-acting elements, gene structures and motifs.

Multi-View Gait Image Generation for Cross-View Gait Recognition.

Gait recognition aims to recognize persons' identities by walking styles. Gait recognition has unique advantages due to its characteristics of non-contact and long-distance compared with face and fingerprint recognition. Cross-view gait recognition is a challenge task because view variance may produce large impact on gait silhouettes.

Canine Olfactory Detection of a Non-Systemic Phytobacterial Citrus Pathogen of International Quarantine Significance.

For millennia humans have benefitted from application of the acute canine sense of smell to hunt, track and find targets of importance. In this report, canines were evaluated for their ability to detect the severe exotic phytobacterial arboreal pathogen pv. (Xcc), which is the causal agent of Asiatic citrus canker (Acc).

Inoculation Period and Citrus Host Effect Establishment of New Infections of ' Liberibacter asiaticus' Transmitted via Vegetative Grafting.

Vegetative grafting is a common method of transmitting and propagating ' Liberibacter asiaticus', the bacterial species accepted as the causal agent of the citrus disease huanglongbing (HLB). Generally, infected tissue that is grafted to a receptor tree remains in place indefinitely to ensure transmission. In this study, individual HLB-symptomatic leaves were grafted as '.

Canine olfactory detection of a vectored phytobacterial pathogen, Liberibacter asiaticus, and integration with disease control.

Early detection and rapid response are crucial to avoid severe epidemics of exotic pathogens. However, most detection methods (molecular, serological, chemical) are logistically limited for large-scale survey of outbreaks due to intrinsic sampling issues and laboratory throughput. Evaluation of 10 canines trained for detection of a severe exotic phytobacterial arboreal pathogen, Liberibacter asiaticus (Las), demonstrated 0.

Genome-wide analysis of polygalacturonase gene family from pear genome and identification of the member involved in pear softening.

Background: Polygalacturonase (PG), as an important hydrolase participating in the degradation of pectin, plays an important role in softening process of fruit. However, information on PG gene family in pear genome and the specific member involved in fruit softening is still rudimentary.

Results: In this study, a total of 61 PG genes, which could be divided into six subclasses, were identified from the pear genome with diverse chromosome locations, gene structures, motifs and cis-acting elements.

Sample Balancing for Deep Learning-Based Visual Recognition.

Sample balancing includes sample selection and sample reweighting. Sample selection aims to remove some bad samples that may lead to bad local optima. Sample reweighting aims to assign optimal weights to samples to improve performance.

Using models to provide rapid programme support for California's efforts to suppress Huanglongbing disease of citrus.

We describe a series of operational questions posed during the state-wide response in California to the arrival of the invasive citrus disease Huanglongbing. The response is coordinated by an elected committee from the citrus industry and operates in collaboration with the California Department of Food and Agriculture, which gives it regulatory authority to enforce the removal of infected trees. The paper reviews how surveillance for disease and resource allocation between detection and delimitation have been addressed, based on epidemiological principles.

A probabilistic census-travel model to predict introduction sites of exotic plant, animal and human pathogens.

International travel offers an extensive network for new and recurring human-mediated introductions of exotic infectious pathogens and biota, freeing geographical constraints. We present a predictive census-travel model that integrates international travel with endpoint census data and epidemiological characteristics to predict points of introduction. Population demographics, inbound and outbound travel patterns, and quantification of source strength by country are combined to estimate and rank risk of introduction at user-scalable land parcel areas (e.

Effect of phosphate on amorphous iron mineral generation and arsenic behavior in paddy soils.

Arsenic (As) contamination was detected in paddy soils of Guangdong, China due to mining and weathering processes. Furthermore, As may be released into the soil and irrigation water during the application of phosphate (P). In this study, As behavior was assessed in three paddy soils (S6, S8 and TR) along the Hengshi river using batch and circular flow experiments with different phosphate application doses (0, 1, 5, 10, 50, 100 mg/L).

Characterization of the Genes Involved in Malic Acid Metabolism from Pear Fruit and Their Expression Profile after Postharvest 1-MCP/Ethrel Treatment.

In this study, five genes involved in malic acid (MA) metabolism, including a cytosolic NAD-dependent malate dehydrogenase gene ( cyNAD-MDH), a cytosolic NADP-dependent malic enzyme gene ( cyNADP-ME), two vacuolar H-ATPase genes ( vVAtp1 and vVAtp2), and one vacuolar inorganic pyrophosphatase gene ( vVPp), were characterized from pear fruit based on bioinformatic and experimental analysis. Their expression profile in "Housui" pear was tissue-specific, and their expression patterns during fruit development were diverse. During "Housui" pear storage, MA content decreased, which was associated with the downregulated transcripts of MA metabolism-related genes and cyNAD-MDH activity and higher cyNADP-ME activity.

Molecular mechanisms underlying heat or tetracycline treatments for citrus HLB control.

Huanglongbing (HLB), a destructive plant bacterial disease, severely impedes worldwide citrus production. In our previous reports, we revealed the molecular mechanisms of host plant responses that underlie thermotherapy against HLB. In this study, we investigated the molecular mechanism underlying heat or tetracycline treatments on the HLB bacterium, ' Liberibacter asiaticus' (Las) by focusing on Las prophage/phage conversion under stress conditions.