Association of gestational hepatitis B virus infection and antiviral therapy with pregnancy outcomes: A retrospective study.

Objective: To explore the relationships between gestational hepatitis B virus (HBV) infection, antiviral therapy, and pregnancy outcomes.

Methods: We retrospectively selected hepatitis B surface antigen (HBsAg)-positive pregnant women hospitalized for delivery at Fujian Medical University Affiliated Hospital from October 1, 2016 to October 1, 2020. The control group included randomly selected healthy pregnant women hospitalized for delivery during the same time.

Genetic algorithm-based semisupervised convolutional neural network for real-time monitoring of Escherichia coli fermentation of recombinant protein production using a Raman sensor.

As a non-destructive sensing technique, Raman spectroscopy is often combined with regression models for real-time detection of key components in microbial cultivation processes. However, achieving accurate model predictions often requires a large amount of offline measurement data for training, which is both time-consuming and labor-intensive. In order to overcome the limitations of traditional models that rely on large datasets and complex spectral preprocessing, in addition to the difficulty of training models with limited samples, we have explored a genetic algorithm-based semi-supervised convolutional neural network (GA-SCNN).

Biodegradable Hydrogen-Bonded Organic Framework for Cytosolic Protein Delivery.

Hydrogen-bonded organic frameworks (HOFs) are a novel class of porous nanomaterials that show great potential for intracellular delivery of protein therapeutics. However, the inherent challenges in interfacing protein with HOFs, and the need for spatiotemporally controlling the release of protein within cells, have constrained their therapeutic potential. In this study, we report novel biodegradable hydrogen-bonded organic frameworks, termed DS-HOFs, specially designed for the cytosolic delivery of protein therapeutics in cancer cells.

Transamination of Aromatic Aldehydes to Primary Arylmethylamines.

In the presence of 2-amino-2-phenylpropanoate salt ( or ) as the amine source, aromatic aldehydes underwent decarboxylative transamination under very mild conditions to produce a variety of arylmethylamines in 44-99% yields. The work has provided an efficient new method for the synthesis of primary arylmethylamines.

Asymmetric Biomimetic Transamination of Trifluoromethyl Ketones.

In the presence of chiral pyridoxamine as the catalyst and 2,2-diphenylglycine () as the amine source, asymmetric biomimetic transamination of trifluoromethyl ketones produces optically active α-trifluoromethyl amines in 81-98% yields with 88-95% ee's under mild conditions.

Orthogonal Chemical Activation of Enzyme-Inducible CRISPR/Cas9 for Cell-Selective Genome Editing.

The precision and therapeutic potential of CRISPR/Cas9 genome editing are greatly challenged by the less control over Cas9-mediated DNA cleavage. Herein, we introduce a conditional and cell-selective genome editing system controlled by disease-associated enzymes, termed enzyme-inducible CRISPR (eiCRISPR). eiCRISPR comprises Cas9 protein, a self-blocked inactive single-guide RNA (bsgRNA), and a chemically caged deoxyribozyme (DNAzyme) that activates bsgRNA and eiCRISPR in a controllable manner.

High-efficiency modulation of broadband polarization conversion with a reconfigurable chiral metasurface.

We demonstrate an electrically biased reconfigurable chiral metasurface for controlling the polarization conversion and asymmetric transmission in a broadband manner. The reconfigurable metasurface is constructed with coupled three-layer complementary split-ring resonator (CSRR) arrays and is loaded with tunable electronic components to achieve dynamic control of reconfigurable chiral coupling in the CSRRs by simply tuning the external voltage on the structure. It is found that the polarization conversion in the metasurface can be effectively and continuously tuned in both experiments and simulations in a broadband frequency range.

Integration of Palladium Nanoparticles with Surface Engineered Metal-Organic Frameworks for Cell-Selective Bioorthogonal Catalysis and Protein Activity Regulation.

Bioorthogonal catalysis provides a powerful tool to perform non-natural chemical reactions in living systems to dissect complex intracellular processes. Its potency to precisely regulate cellular function, however, is limited by the lack of bioorthogonal catalysts with cell selectivity. Herein, we report that palladium nanoparticles deposited on metal-organic frameworks, Pd@UiO-66, are highly efficient for intracellular bioorthogonal catalysis.

Fano-Resonant Hybrid Metamaterial for Enhanced Nonlinear Tunability and Hysteresis Behavior.

Artificial resonant metamaterial with subwavelength localized filed is promising for advanced nonlinear photonic applications. In this article, we demonstrate enhanced nonlinear frequency-agile response and hysteresis tunability in a Fano-resonant hybrid metamaterial. A ceramic cuboid is electromagnetically coupled with metal cut-wire structure to excite the high-Q Fano-resonant mode in the dielectric/metal hybrid metamaterial.

Asymmetric biomimetic transamination of α-keto amides to peptides.

Peptides are important compounds with broad applications in many areas. Asymmetric transamination of α-keto amides can provide an efficient strategy to synthesize peptides, however, the process has not been well developed yet and still remains a great challenge in both enzymatic and catalytic chemistry. For biological transamination, the high activity is attributed to manifold structural and electronic factors of transaminases.

EIA metamaterials based on hybrid metal/dielectric structures with dark-mode-enhanced absorption.

Metamaterial analogue of electromagnetically induced absorption (EIA) has promising applications in spectroscopy and sensing. Here we propose an EIA metamaterial based on hybrid metal/dielectric structures, which are composed of a metallic wire and a dielectric block, and investigate the EIA-like effect by simulations, experiments, and the two-oscillator model. An EIA-like effect emerges in virtue of the near-field coupling between metallic wire and dielectric block, and the dielectric block exhibiting magnetic dipolar resonance makes a major contribution to the resonance absorption.

Spatiotemporal Delivery of CRISPR/Cas9 Genome Editing Machinery Using Stimuli-Responsive Vehicles.

Recent innovations in genome editing have enabled the precise manipulation of the genetic information of mammalians, and benefitted the development of next-generation gene therapy. Despite these advances, several barriers to the clinical translation of genome editing remain, including the intracellular delivery of genome editing machinery, and the risk of off-target editing effect. Here, we review the recent advance of spatiotemporal delivery of CRISPR/Cas9 genome editing machinery, which is composed of programmable Cas9 nuclease and a single-guide RNA (sgRNA) using stimuli-responsive nanoparticles.

Engineering nucleic acid chemistry for precise and controllable CRISPR/Cas9 genome editing.

The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein 9 (CRISPR/Cas9) genome editing technology is revolutionizing our approach and capability to precisely manipulate the genetic flow of mammalians. The facile programmability of Cas9 protein and guide RNA (gRNA) sequence has recently expanded biomedical application of CRISPR/Cas9 technology from editing mammalian genome to various genetic manipulations. The therapeutic and clinical translation potential of CRISPR/Cas9 genome editing, however, are challenged by its off-target effect and low genome editing efficiency.

Enzyme-Instructed Activation of Pro-protein Therapeutics In Vivo.

The selective and temporal control of protein activity in living cells provides a powerful tool to manipulate cellular function and to develop pro-protein therapeutics (PPT) for targeted therapy. In this work, we reported a facile but general chemical approach to design PPT by modulating protein activity in response to endogenous enzyme of disease cells, and its potential for targeted cancer therapy. We demonstrated that the chemical modification of a protein with quinone propionic acid (QPN), a ligand that could be reduced by tumor-cell-specific NAD(P)H dehydrogenase [quinone] 1 (NQO1), was reversible in the presence of NQO1.

Thermally controllable Mie resonances in a water-based metamaterial.

Active control of metamaterial properties is of great significance for designing miniaturized and versatile devices in practical engineering applications. Taking advantage of the highly temperature-dependent permittivity of water, we demonstrate a water-based metamaterial comprising water cubes with thermally tunable Mie resonances. The dynamic tunability of the water-based metamaterial was investigated via numerical simulations and experiments.

Aminative Umpolung cyclization for synthesis of chiral exocyclic vicinal diamines.

Chiral exocylic vicinal diamines are biologically and chemically important compounds, but they are not easy to make. In this paper, an interesting aminative Umpolung cyclization process has been developed. Aromatic aldehydes 6 bearing an electrophilic chiral sulfinimine group underwent imine formation with 2,2-diphenylglycine (2), decarboxylation, and subsequent Umpolung cyclization, producing various trans-diamines 10 in 84-96% yields with high trans/cis ratios under very mild conditions.

Metropolitan all-pass and inter-city quantum communication network.

We have demonstrated a metropolitan all-pass quantum communication network in field fiber for four nodes. Any two nodes of them can be connected in the network to perform quantum key distribution (QKD). An optical switching module is presented that enables arbitrary 2-connectivity among output ports.

Anti-Ki-67 peptide nucleic acid affects the proliferation and apoptosis of human renal carcinoma cells in vitro.

We treated in vitro human renal carcinoma cells (cell line 786-0) with the lipid-delivered peptide nucleic acids (PNAs) against Ki-67 gene. Corresponding control groups were treated with the antisense oligonucleotides (ASOs) of the same nucleobase sequence, and with mismatched PNAs. In cells treated by anti-Ki-67 PNAs, the Ki-67 expression rate, Ki-67 protein level, cell growth and the DNA synthesis-indicative 3H-thymidine incorporation rate were lower than in the ASO-treated groups, and reduced significantly compared to untreated controls, whereas the rate of apoptosis was markedly increased by PNA treatment.