Arsenate-Induced Changes in Bacterial Metabolite and Lipid Pools during Phosphate Stress.

GW4 is a heterotrophic arsenite-oxidizing bacterium with a high resistance to arsenic toxicity. It is now a model organism for studying the processes of arsenic detoxification and utilization. Previously, we demonstrated that under low-phosphate conditions, arsenate [As(V)] could enhance bacterial growth and be incorporated into biomolecules, including lipids.

Regulation of Class A β-Lactamase CzoA by CzoR and IscR in S44.

A genomic analysis of S44 revealed a gene that encodes a LysR family transcriptional regulator (here named , czo for cefazolin) located upstream of a putative class A β-lactamase encoding gene (here named ). A putative DNA-binding motif of the Fe-S cluster assembly regulator IscR was identified in the - intergenic region. Real-time RT-PCR and fusion expression assays indicated that transcription of and were induced by multiple β-lactams.

Nocardioides immobilis sp. nov., isolated from iron mine soil.

A Gram-positive, non-motile, rod-shaped aerobic bacterial strain FLL521 was isolated from iron mine soil. Phylogenetic trees based on 16S rRNA gene sequences showed that strain FLL521 belonged to genus Nocardioides in family Nocardioidaceae, with the highest sequence identity to Nocardioides panacisoli GSoil 346 (96.3 %).

Global Regulator IscR Positively Contributes to Antimonite Resistance and Oxidation in Comamonas testosteroni S44.

Antimonial compounds can be found as a toxic contaminant in the environment. Knowledge on mechanisms of microbial Sb oxidation and its role in microbial tolerance are limited. Previously, we found that Comamonas testosteroni S44 was resistant to multiple heavy metals and was able to oxidize the toxic antimonite [Sb(III)] to the much less toxic antimonate [Sb(V)].

Draft genome sequence of Cellulomonas carbonis T26(T) and comparative analysis of six Cellulomonas genomes.

Most Cellulomonas strains are cellulolytic and this feature may be applied in straw degradation and bioremediation. In this study, Cellulomonas carbonis T26(T), Cellulomonas bogoriensis DSM 16987(T) and Cellulomonas cellasea 20108(T) were sequenced. Here we described the draft genomic information of C.

Synthesis and biological evaluation of 4-fluoroproline and 4-fluoropyrrolidine-2-acetic acid derivatives as new GABA uptake inhibitors.

Preparation for the N-alkylated derivatives of enantiomerically pure (2S)-4-fluoroproline and (2S)-4-fluoropyrrolidine-2-acetic acid is described. The final compounds were evaluated as potential GAT-1 uptake inhibitors via cultured cell lines expressing mouse GAT-1. Compared with their corresponding 4-hydroxy compounds, these derivatives exhibited slight improvement on their inhibitory potency, but still much weaker than their corresponding compounds with no substituents at the C-4 of the pyrrolidine moiety, with the most potent affinity being about 1/15 fold as that of Tiagabine.