Successful application of microspore culture by adding kinetin and methylene blue to improve microspore embryogenesis in Chinese kale breeding.

Microspore culture is an efficient and rapid method that produces doubled haploid (DH) lines for hybrid breeding in crops and vegetables. However, the low frequency of microspore embryogenesis and spontaneous diploidization in Chinese kale still require improvement. In the present work, an efficient microspore culture protocol was constructed and used for DH producing in Chinese kale breeding.

TFEB activator protects against ethanol toxicity-induced cardiac injury by restoring mitophagy and autophagic flux.

Excessive alcohol consumption is a major cause of alcoholic cardiomyopathy (ACM) and myocardial injury. This study aims to investigate the role of transcription factor EB (TFEB) in ethanol-induced cardiac anomalies using a murine model, AC16 human cardiomyocytes, and human plasma. Wild-type mice treated with a TFEB activator (Compound 1) or vehicle (25 mg/kg/d) were challenged with or without ethanol (3 g/kg/d, i.

Characterization of volatile aroma compounds in pak choi.

Aroma is a key sensory factor in the flavor evaluation of pak choi ( L. ssp. var.

Numerical simulation of neural excitation during brain tumor ablation by microsecond pulses.

Replacing monopolar pulse with bipolar pulses of the same energized time can minimize unnecessary neurological side effects during irreversible electroporation (IRE). An improved neural excitation model that considers dynamic conductivity and thermal effects during brain tumor IRE ablation was proposed for the first time in this study. Nerve fiber excitation during IRE ablation by applying a monopolar pulse (100 μs) and a burst of bipolar pulses (energized time of 100 μs with both the sub-pulse length and interphase delay of 1 μs) was investigated.

Considering Joule heating in coupled electroporation and electrodeformation modeling of glioblastoma cells.

Cells exposed to a pulsed electric field undergo electroporation(EP) and electrodeformation(ED) under electric field stress, and a coupled model of EP and ED of glioblastoma(GBM) taking into account Joule heating is proposed. The model geometry is extracted from real cell boundaries, and the effects of Joule heating-induced temperature rise on the EP and ED processes are considered. The results show that the temperature rise will increase the cell's local conductivity, leading to a decrease in the transmembrane potential(TMP).

Preparation and Characterization of Nano-FeO and Its Application for C18-Functionalized Magnetic Nanomaterials Used as Chromatographic Packing Materials.

A new type of magnetic nanomaterial with FeO as the core and organic polymer as the shell was synthesized by seed emulsion polymerization. This material not only overcomes the problem of insufficient mechanical strength of the organic polymer, it also solves the problem that FeO is prone to oxidation and agglomeration. In order to make the particle size of FeO meet the requirement of the seed, the solvothermal method was used to prepare FeO.

Early childhood health shocks, classroom environment, and social-emotional outcomes.

This study finds that experiencing early childhood health shocks before primary school exerts negative externalities in the middle school classroom. Students randomly assigned to classrooms in which more classmates experienced early childhood health shocks are more likely to have worse social-emotional outcomes, including emotional distress, less engagement with school activities, and poor social acclimation with classmates. These effects operate by damaging inter-student relationships, and are unlikely to operate by affecting teachers' behaviors or preferences.

Increased 99mTc-Sestamibi Activity in Giant Cell Tumor of Bone.

A 99mTc-sestamibi parathyroid scintigraphy was performed in a 27-year-old woman to evaluate possible hyperparathyroidism. The images did not identify any parathyroid abnormality. However, an intense activity was noted in the proximal left upper extremity, which was confirmed as giant cell tumor of the proximal left humerus by pathological examination.

Dentists have a high occupational risk of neck disorders with impact on somatosensory function and neck mobility.

Objectives: Musculoskeletal disorders (MSDs) in the neck and shoulder region may be associated with significant impairment of quality of life and well-being. The study was to determine the prevalence of painful MSDs in Chinese dentists and evaluate somatosensory function and neck mobility compared with non-dental professional controls.

Methods: One hundred dentists (age: 36.

Parathyroid hormone promotes the osteogenesis of lipopolysaccharide-induced human bone marrow mesenchymal stem cells through the JNK MAPK pathway.

Periodontitis is a series of inflammatory processes caused by bacterial infection. Parathyroid hormone (PTH) plays a critical role in bone remodeling. The present study aimed to investigate the influences of PTH on human bone marrow mesenchymal stem cells (HBMSCs) pretreated with lipopolysaccharide (LPS).

The curse of modernization? Western fast food and Chinese children's weight.

The income-adjusted price of fast food in China is five times more than in the United States, yet we show that the introduction of Western fast-food restaurants to China still leads to significant weight gain in children. Using the community-year-level presence of Western fast-food outlets, difference-in-differences estimations find a 4.8-percentage-point increase in the prevalence of overweight/obese children after controlling for child and year fixed effects.

Effect of photobiomodulation therapy on painful temporomandibular disorders.

To evaluate the effect of photobiomodulation therapy (PBMT) on painful temporomandibular disorders (TMD) patients in a randomized, double-blinded, placebo-controlled manner. Participants were divided into a masseter myalgia group (n = 88) and a temporomandibular joint (TMJ) arthralgia group (n = 87) according to the Diagnostic Criteria for Temporomandibular Disorders (DC/TMD). Both groups randomly received PBMT or placebo treatment once a day for 7 consecutive days, one session.

Human amnion-derived mesenchymal stem cells promote osteogenic differentiation of lipopolysaccharide-induced human bone marrow mesenchymal stem cells via ANRIL/miR-125a/APC axis.

Background And Aim: Periodontitis is a chronic inflammatory disease inducing the absorption of alveolar bone and leading to tooth loss. Human amnion-derived mesenchymal stem cells (HAMSCs) have been used for studying inflammatory processes. This study aimed to explore the role of long noncoding RNA (lncRNA) antisense noncoding RNA in the INK4 locus (ANRIL) in HAMSC-driven osteogenesis in lipopolysaccharide (LPS)-induced human bone marrow mesenchymal stem cells (HBMSCs).

The shattered "Iron Rice Bowl": Intergenerational effects of Chinese State-Owned Enterprise reform.

Reform of the Chinese State-Owned Enterprise (SOE) sector in the late 1990s triggered massive layoffs (34 million employees) and marked the end of the "Iron Rice Bowl" guarantee of employment security for the remaining 67 million workers. An expanding international literature has documented the adverse health impacts of economic insecurity on adults, but has typically neglected children. This paper uses the natural experiment of SOE reform to explore the causal relationship between increased parental economic insecurity and children's BMI Z-score.

Comparative Outcomes of Block and Cancellous Iliac Bone Grafting in Older Unilateral Alveolar Cleft Patients.

Objective: To compare osseous outcomes of block and cancellous iliac bone grafting in older unilateral alveolar cleft patients.

Design: Retrospective and observational follow-up study.

Setting: Cleft Lip and Palate Centre, Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, China.

Inactivation of RUNX3 protein expression in tongue squamous cell carcinoma and its association with clinicopathological characteristics.

The function of runt‑related transcription factor 3 (RUNX3) in oral cancer remains controversial. The present study aimed to determine the status of RUNX3 protein expression and its association with clinicopathological characteristics in tongue squamous cell carcinomas (SCC). The present study used three pairs of tongue SCC and non‑cancerous tissues to assess RUNX3 protein expression by western blot analysis, and two tongue SCC cell lines to determine RUNX3 protein localization by immunofluorescence and immunocytochemistry.

Reliability of Mechanical Sensitivity Mapping in the Orofacial Region of Healthy Chinese Individuals: Towards Standardized Assessment of Somatosensory Function.

Aims: To investigate the test-retest reliability of mechanical sensitivity mapping in the masseter and temporomandibular joint (TMJ) regions between sessions, days, and examiners with a fixed and standardized pressure stimulus, as well as to compare mechanical sensitivity between sides and sites.

Methods: A total of 20 healthy young volunteers participated. Pressure stimulation was applied to 15 sites in the masseter region with a Palpeter device of 1.

Radiation exposure triggers the progression of triple negative breast cancer via stabilizing ZEB1.

Our present study confirmed radiation can promote the in vitro migration and invasion of triple negative breast cancer (TNBC) cells and increase the expression of epithelial-mesenchymal transition (EMT) related transcription factor ZEB1, while had no effect on Snail, Slug or Twist. Knockdown of ZEB1 attenuated radiation induced cell migration and invasion, suggesting that ZEB1 is essential for radiation induced progression of TNBC. Radiation increased the protein stability of ZEB1, while had no effect on its mRNA expression.

Erratum: Mismatched intratumoral distribution of [18F] fluorodeoxyglucose and 3'-deoxy-3'-[18F] fluorothymidine in patients with lung cancer.

[This corrects the article DOI: 10.3892/ol.2017.

Mismatched intratumoral distribution of [F] fluorodeoxyglucose and 3'-deoxy-3'-[F] fluorothymidine in patients with lung cancer.

In a mouse model of human lung cancer, intratumoral distribution between 3'-deoxy-3'-[F] fluorothymidine (F-FLT) and [F] fluorodeoxyglucose (F-FDG) was mutually exclusive. F-FLT primarily accumulated in proliferating cancer cells, whereas F-FDG accumulated in hypoxic cancer cells. The aim of the present study was to evaluate these preclinical findings in patients with lung cancer.

RUNX3 plays a tumor suppressor role by inhibiting cell migration, invasion and angiogenesis in oral squamous cell carcinoma.

Although aberrant expression of Runt-related transcription factor 3 (RUNX3) contributes to tumor progression and metastasis in a number of carcinomas, the status of RUNX3 and its correlation with prognosis in oral squamous cell carcinomas (OSCC) are still controversial. The aim of present study was to investigate the function of RUNX3 in OSCC and the underlying molecular mechanisms. Tissue microarray (TMA) consisting of 232 OSCC specimens was used to detect the expression of RUNX3 by immunohistochemistry method.