Involvement of Mycobacterium smegmatis small noncoding RNA B11 in triacylglycerol accumulation and altered cell wall permeability.

Background: Pathways involving triacylglycerol (TAG) accumulation are thought to play a crucial regulatory role in bacterial growth and metabolism. Despite this understanding, little is known about the biological functions and regulatory mechanisms of small RNAs in Mycobacterium. Mycobacterium smegmatis (M.

Pilot Study of Speech and Ventilation Quality of Life After Cleft Palate Repair in Chinese Patients.

Objective (s): This pilot study aimed to assess the quality of life (QoL) in Chinese patients with velopharyngeal competence (VPC) after primary cleft palate surgery or after secondary posterior pharyngeal flap (PPF) surgery.

Methods: A two-center cross-sectional study was conducted in two stomatological hospitals in China between December 2021 and December 2022. A total of 68 patients and healthy volunteers were enrolled in this study, categorized into the control group, the group of patients with VPC after primary cleft palate repair, and the group of patients with VPC after secondary PPF.

Ovarian adenomyoma: a case report.

Introduction: Ovarian adenomyoma is a rare gynecological tumor with a high misdiagnosis rate, leading many patients to undergo unnecessary surgeries that may affect fertility. Menstrual abdominal pain is the most common symptom, and auxiliary examinations often cannot clarify its nature. It often relies on intraoperative diagnosis, and surgical resection can achieve good therapeutic effects.

Metabolic Blockade-Based Genome Mining of SCSIO 07745: Discovery and Biosynthetic Pathway of Aminoquinolinone Alkaloids Bearing 6/6/5 Tricyclic and 6/6/6/5 Tetracyclic Scaffolds.

Metabolic blockade-based genome mining of the marine sediment-derived SCSIO 07745 led to the discovery of 11 novel aminoquinolinone alkaloids, oxazoquinolinones A-J (-), characterized by an oxazolidone[3,2-α]quinoline-5,8-dione scaffold, and oxazoquinolinone K (), featuring an unprecedented fused 6/6/6/5 tetracyclic core ring system. Additionally, 5 new biosynthetic intermediates or shunt products (-) and a known metabolite sannanine () were identified. Their structures were elucidated by extensive spectroscopic analyses and a comparison of electronic circular dichroism and single-crystal X-ray diffraction.

Copper-Catalyzed Enantioconvergent Radical C(sp)-N Cross-Coupling to Access Chiral α-Amino-β-lactams.

A copper-catalyzed enantioconvergent radical C(sp)-N cross-coupling of racemic tertiary α-bromo-β-lactams with aromatic amines is developed under mild thermal reaction conditions. The use of a sterically demanded oxazoline-derived sulfonamide N,N,N-ligand is crucial for the reaction initiation and effective enantio-discrimination of the azetidinone-derived cyclic alkyl radicals. The strategy provides an attractive approach to access chiral α-amino-β-lactams, an important structural motif in many biologically active molecules.

Manipulation and applications of ultrafast all-optical switching based on transient absorption and dispersion in KTN crystals.

Potassium tantalate niobate (KTN) represents a noteworthy category of optical crystals known for their superior nonlinear optical properties. In this study, we conducted measurements of femtosecond time-resolved transient absorption (TA) spectra in KTaNbO crystals. Notably, a rapid and pronounced "plateau" phase, ∼1.

Synergistic dual built-in electric fields in 1T-MoS/NiS/LDH for efficient electrocatalytic overall water splitting reactions.

Designing cost-effective electrocatalysts for water decomposition is crucial for achieving environmental-friendly hydrogen production. A transition metal sulfide/hydroxide electrocatalyst (1T-MoS/NiS/LDH) with double heterogeneous interfaces was developed through a two-step hydrothermal assisted electrodeposition method. The presence of the two built-in electric fields not only accelerated the charge transfer at the interface, but also enhanced the adsorption of the reactants and intermediate groups, and therefore improved the reaction rate and overall catalytic performance.

Swing Origami-Structure-Based Triboelectric Nanogenerator for Harvesting Blue Energy toward Marine Environmental Applications.

The appearance of triboelectric nanogenerators (TENG) provides a promising energy technology for harvesting abundant water wave energy. Here, the design and fabrication of a swinging origami-structured TENG (SO-TENG) tailored specifically for water wave energy harvesting are presented. The design incorporates an oscillating structure weighted at the bottom, inducing reciprocating motion propelled by the inertia of passing water waves.

Copper-Catalyzed Enantioconvergent Radical -Alkylation of Diverse (Hetero)aromatic Amines.

The 3d transition metal-catalyzed enantioconvergent radical cross-coupling provides a powerful tool for chiral molecule synthesis. In the classic mechanism, the bond formation relies on the interaction between nucleophile-sequestered metal complexes and radicals, limiting the nucleophile scope to sterically uncongested ones. The coupling of sterically congested nucleophiles poses a significant challenge due to difficulties in transmetalation, restricting the reaction generality.

Copper-Catalyzed Enantioconvergent Radical C(sp)-N Cross-Coupling of Activated Racemic Alkyl Halides with (Hetero)aromatic Amines under Ambient Conditions.

The enantioconvergent C(sp)-N cross-coupling of racemic alkyl halides with (hetero)aromatic amines represents an ideal means to afford enantioenriched -alkyl (hetero)aromatic amines yet has remained unexplored due to the catalyst poisoning specifically for strong-coordinating heteroaromatic amines. Here, we demonstrate a copper-catalyzed enantioconvergent radical C(sp)-N cross-coupling of activated racemic alkyl halides with (hetero)aromatic amines under ambient conditions. The key to success is the judicious selection of appropriate multidentate anionic ligands through readily fine-tuning both electronic and steric properties for the formation of a stable and rigid chelating Cu complex.

Increasing clinical medical service satisfaction: An investigation into the impacts of Physicians' use of clinical decision-making support AI on patients' service satisfaction.

Background: The medical industry is one of the key industries for the application of artificial intelligence (AI). Although it is believed that the combination of CDSS and physicians could improve the medical service, there are still many concerns about the usage of CDSS. Based on these concerns, limited studies have answered the question that when a physician makes decision independently or with AI's help, will there be any differences in patients' satisfaction with the medical service?

Methods: This study uses the service fairness theory as a theoretical lens and employs three vignette experiments to address this research gap.

Enantioconvergent Cu-catalysed N-alkylation of aliphatic amines.

Chiral amines are commonly used in the pharmaceutical and agrochemical industries. The strong demand for unnatural chiral amines has driven the development of catalytic asymmetric methods. Although the N-alkylation of aliphatic amines with alkyl halides has been widely adopted for over 100 years, catalyst poisoning and unfettered reactivity have been preventing the development of a catalyst-controlled enantioselective version.

Evaluation of the effect of a resorbable membrane on the closure of palatal fistulas.

Purpose: A palatal fistula following the closure of palatal clefts remains a difficult clinical complication. Surgical treatment of fistulas is often complicated, with high recurrence rates. We present our results of fistula closure augmented with GTR, a resorbable membrane designed to promote guided tissue regeneration.

Comparison of velopharyngeal morphology of two palatoplasty techniques in patients with hard and soft cleft palate.

Purpose: The study aims to compare the velopharyngeal morphology of hard and soft cleft palate (HSCP) patients after Furlow and Sommerlad palatoplasty.

Patients And Methods: A total of 51 patients (20 cases in Furlow palatoplasty group, 16 cases in Sommerlad palatoplasty group and 15 normal children in the control group) were included in our study. Velopharyngeal function and speech outcomes of patients with HSCP who had either Furlow palatoplasty or Sommerlad palatoplasty for cleft palate repair were evaluated by perceptual speech assessment (PSA), lateral cephalometric radiographs and nasopharyngoscopy.

Transcriptome sequencing analysis of the role of β-catenin in F-actin reorganization in embryonic palatal mesenchymal cells.

Background: Palatogenesis is a highly regulated and coordinated developmental process that is coordinated by multiple transcription factors and signaling pathways. Our previous studies identified that defective palatal shelf reorientation due to aberrant mesenchymal β-catenin signaling is associated with Filamentous actin (F-actin) dysregulation. Herein, the underlying mechanism of mesenchymal β-catenin in regulating F-actin cytoskeleton reorganization is further investigated.

Excitation-Dependence of Excited-State Dynamics and Vibrational Relaxation of Lutein Explored by Multiplex Transient Grating.

Multiplex transient grating (MTG) spectroscopy was applied to lutein in ethanol to investigate the excitation-energy dependence of the excited-state dynamics and vibrational relaxation. The transient spectra obtained upon low (480 nm) and high-energy (380 nm) excitation both recorded a strong excited-state absorption (ESA) of S → S as well as a broad band in the blue wavelength that was previously proposed as the S* state. By means of Gaussian decomposition and global fitting of the ESA band, a long-time component assigned to the triplet state was derived from the kinetic trace of 480 nm excitation.

Collision-free emergency planning and control methods for CAVs considering intentions of surrounding vehicles.

Autonomous emergency braking (AEB) systems are able to control vehicles as needed to avoid vehicle rear-end collisions. However, these systems are ineffective in scenarios with laterally cut-in vehicles and rapidly-changing dangerous scenes. This paper proposes a novel collision-free emergency braking system (CFEBS) that can enable intelligent connected vehicles (CAVs) to plan and execute a more conservative safety trajectory for the braking process in dangerous scenes by considering the longitudinal and lateral motion intentions of the surrounding vehicles.

Gossypol Broadly Inhibits Coronaviruses by Targeting RNA-Dependent RNA Polymerases.

Outbreaks of coronaviruses (CoVs), especially severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have posed serious threats to humans and animals, which urgently calls for effective broad-spectrum antivirals. RNA-dependent RNA polymerase (RdRp) plays an essential role in viral RNA synthesis and is an ideal pan-coronaviral therapeutic target. Herein, based on cryo-electron microscopy and biochemical approaches, gossypol (GOS) is identified from 881 natural products to directly block SARS-CoV-2 RdRp, thus inhibiting SARS-CoV-2 replication in both cellular and mouse infection models.

Mesenchymal β-catenin signaling affects palatogenesis by regulating α-actinin-4 and F-actin.

Objective: Our previous research have found that mesenchymal β-catenin may be involved in palatal shelf (PS) elevation by regulating F-actin. Here, we further investigated the exact mechanism of β-catenin/F-actin in the PS mesenchyme to regulate palatal reorientation.

Materials And Methods: (1) Firstly, Ctnnb1 (β-catenin) mice were conditionally overexpressed in the palatal mesenchyme by crossing with the Sox9-creER mice (induced by Tamoxifen injections); (2) Subsequently, histology and immunohistochemistry were used to characterize the variations of PS morphology and expression of key molecules associated with developmental process; (3) Finally, experiments in vivo and ex vivo were employed to identify the critical mechanisms in β-catenin silenced and overexpressed models.

Effects of Acute Interval Exercise on Arterial Stiffness and Cardiovascular Autonomic Regulatory Responses: A Narrative Review of Potential Impacts of Aging.

The physiological changes associated with aging deleteriously impact cardiovascular function and regulation and therefore increase the risk of developing cardiovascular disease. There is substantial evidence that changes in the autonomic nervous system and arterial stiffness play an important role in the development of cardiovascular disease during the aging process. Exercise is known to be effective in improving autonomic regulation and arterial vascular compliance, but differences in the type and intensity of exercise can have varying degrees of impact on vascular regulatory responses and autonomic function.

A fourth dose of Omicron RBD vaccine enhances broad neutralization against SARS-CoV-2 variants including BA.1 and BA.2 in vaccinated mice.

The SARS-CoV-2 vaccines have been widely used to build an immunologic barrier in the population against the COVID-19 pandemic. However, a newly emerging Omicron variant, including BA.1, BA.

Research on energy consumption evaluation of electric vehicles for thermal comfort.

This paper presents an energy consumption evaluation method for electric vehicles under different cooling and heating conditions. First, using the actual driving test data of electric vehicles, the weight coefficient of the energy consumption value per 100 km of the electric vehicle undercooling, heating, and non-cooling heating is obtained by the least-square method in the comprehensive energy consumption value of the electric vehicle per 100 km throughout the year. Then, the above weight coefficients are combined with the test results of the bench test to obtain the comprehensive energy consumption per 100 km of the electric vehicle in the whole year.

Sequential immunization with SARS-CoV-2 RBD vaccine induces potent and broad neutralization against variants in mice.

The current COVID-19 pandemic caused by constantly emerging SARS-CoV-2 variants still poses a threat to public health worldwide. Effective next-generation vaccines and optimized booster vaccination strategies are urgently needed. Here, we sequentially immunized mice with a SARS-CoV-2 wild-type inactivated vaccine and a heterologous mutant RBD vaccine, and then evaluated their neutralizing antibody responses against variants including Beta, Delta, Alpha, Iota, Kappa, and A.

Identification of a Covalent Importin-5 Inhibitor, Goyazensolide, from a Collective Synthesis of Furanoheliangolides.

Sesquiterpenes are a rich source of covalent inhibitors with a long history in traditional medicine and include several important therapeutics and tool compounds. Herein, we report the total synthesis of 16 sesquiterpene lactones via a build/couple/pair strategy, including goyasensolide. Using an alkyne-tagged cellular probe and proteomics analysis, we discovered that goyazensolide selectively targets the oncoprotein importin-5 (IPO5) for covalent engagement.