Analysis of protein glycosylation and phosphorylation using liquid phase separation, protein microarray technology, and mass spectrometry.

Protein glycosylation and phosphorylation are very common posttranslational modifications. The alteration of these modifications in cancer cells is closely related to the onset and progression of cancer and other disease states. In this protocol, strategies for monitoring the changes in protein glycosylation and phosphorylation in serum or tissue cells on a global scale and specifically characterizing these alterations are included.

Conductive polymer as a controlled microenvironment for the potentiometric high-throughput evaluation of ionic liquid cell toxicity.

This paper presents both biological and potentiometric evaluations of the cell toxicity of a widely used ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim]BF(4)), to Chinese hamster lung fibroblast cells (V79 cell line). The innovative potentiometric study takes advantage of the unique properties of conductive polymer polypyrrole (PPY) for the potentiometric evaluation of cell toxicity of [bmim]BF(4) to the V79 cells in a real-time, noninvasive and high-throughput manner. The conductive polymer PPY provides a controlled microenvironment that allows the quantitative release of the anions of the ionic liquids into the cells being monitored in real time and noninvasively.

Glycoprotein microarrays with multi-lectin detection: unique lectin binding patterns as a tool for classifying normal, chronic pancreatitis and pancreatic cancer sera.

Pancreatic cancer is the fourth leading cause of cancer-related death in the United States, with a 5-year survival rate of less than 4%. Effective early detection and screening are currently not available, and tumors are typically diagnosed at a late stage, frequently after metastasis. Existing clinical markers of pancreatic cancer lack specificity, as they are also found in inflammatory diseases of the pancreas and biliary tract.

N-linked glycosylation profiling of pancreatic cancer serum using capillary liquid phase separation coupled with mass spectrometric analysis.

Glycoproteins play important roles in various biological processes including intracellular transport, cell recognition, and cell-cell interactions. The change of the cellular glycosylation profile may have profound effects on cellular homeostasis and malignancy. Therefore, we have developed a sensitive screening approach for the comprehensive analysis of N-glycans and glycosylation sites on human serum proteins.