Shared and specific biological signalling pathways for diabetic retinopathy, peripheral neuropathy and nephropathy by high-throughput sequencing analysis.

Objectives: We aimed to explore the shared and specific signalling pathways involved in diabetic retinopathy (DR), diabetic peripheral neuropathy (DPN) and diabetic nephropathy (DN).

Methods: Differentially expressed mRNAs and lncRNAs were identified by high-throughput sequencing. Subsequently, functional enrichment analysis, protein-protein interaction (PPI) analysis and lncRNAs-mRNAs networks were conducted to determine the pathogenic mechanisms underlying DR, DPN and DN.

Involvement of CircRNA Expression Profile in Diabetic Retinopathy and Its Potential Diagnostic Value.

Circular RNAs (circRNAs), a class of non-coding and undegradable RNAs, play many pathological functions by acting as miRNA sponges, interacting with RNA-binding proteins, and others. The recent literature indicates that circRNAs possess the advanced superiority for the early screening of diabetic retinopathy (DR). CircRNA sources of peripheral blood mononuclear cells (PBMCs) from healthy controls ( = 4), diabetes mellitus patients (DM) ( = 4), and DR patients ( = 4) were extracted for circular RNA microarray analysis.

The Efficacy of Rituximab Combined with 131I for Ophthalmic Outcomes of Graves' Ophthalmopathy Patients.

Purpose: The purpose of this study is to examine the effectiveness of introducing both rituximab (RTX) and 131I for active Graves' ophthalmopathy (GO) with hyperthyroidism.

Methods: In total, 217 patients suffering from active GO with hyperthyroidism were included in this research. All subjects were randomly assigned to 3 groups.

A novel CRX mutation by whole-exome sequencing in an autosomal dominant cone-rod dystrophy pedigree.

Aim: To identify the disease-causing gene mutation in a Chinese pedigree with autosomal dominant cone-rod dystrophy (adCORD).

Methods: A southern Chinese adCORD pedigree including 9 affected individuals was studied. Whole-exome sequencing (WES), coupling the Agilent whole-exome capture system to the Illumina HiSeq 2000 DNA sequencing platform was used to search the specific gene mutation in 3 affected family members and 1 unaffected member.

A comparison of ultrasound and magnetic resonance imaging to assess visceral fat in the metabolic syndrome.

Visceral adipose tissue (VAT) plays a key role in the metabolic syndrome. Easy detection of VAT could be an important tool to increase understanding of the metabolic syndrome. To study the relationship between the area of the inferior part of the perirenal fat (AIPPF) and anthropometric, imaging and cardiovascular risk factors of metabolic syndrome, seventy two subjects with metabolic syndrome were recruited including 44 men and 28 women (age: 26-68 yr).

[The effect of kanglaite injection(KLT) on the proliferation and telomerase activity of rat mesangial cells].

Objective: To observe the effect of Kanglaite injection(KLT) on the proliferation and telomerase activity of mesangial cells in rats.

Method: MTT, telomere repeat amplification protocal (TRAP), ELISA, PAGE and silver-stain were applied to detect the growth rate and telomerase activity of MC after stimulation of KLT and IL-1.

Result: The growth rate of MC was enhanced by IL-1 stimulation, which was accompanied with a redection of the activity of telomerase.