E93 promotes transcription of RHG genes to initiate apoptosis during Drosophila salivary gland metamorphosis.

20-hydroxyecdysone (20E) induced transcription factor E93 is important for larval-adult transition, which functions in programmed cell death of larval obsolete tissues, and the formation of adult new tissues. However, the apoptosis-related genes directly regulated by E93 are still ambiguous. In this study, an E93 mutation fly strain was obtained by clustered regularly interspaced palindromic repeats (CRISPR) / CRISPR-associated protein 9-mediated long exon deletion to investigate whether and how E93 induces apoptosis during larval tissues metamorphosis.

A Blueprint of Microstructures and Stage-Specific Transcriptome Dynamics of Cuticle Formation in .

Insect cuticle is critical for the environmental adaptability and insecticide resistance of insects. However, there is no clear understanding of the structure and protein components of the cuticle during each developmental stage of holometabolous insects, and knowledge about the protein components within each layer is vague. We conducted serial sectioning, cuticular structure analysis, and transcriptome sequencing of the larval, pupal, and adult cuticles of .

Regulation of antimicrobial peptide genes via insulin-like signaling pathway in the silkworm Bombyx mori.

Antimicrobial peptides (AMPs) are important effector molecules of insect humoral immunity, and expression of AMPs is mainly regulated by the Toll and immune deficiency (IMD) pathways. FoxO, a key downstream regulator of the insulin-like signaling (ILS) pathway, has been recently reported to be involved in the regulation of AMPs in Drosophila melanogaster. In the present study, we investigated AMP gene expression and the regulation pathway controlled by the starvation in the silkworm Bombyx mori.

The Structural Variation Is Associated with the Embryonic Lethality of a Novel Red Egg Mutant Fuyin-lre of Silkworm, Bombyx mori.

Bombyx mori presents several types of egg color mutations, all of which have been extensively discussed in sericulture. While the red egg mutation has been previously observed, lethal red-egg mutants have not been reported. In the present work, the red egg mutant Fuyin-lre (Fuyin-lethal red egg) was discovered from the Fuyin germplasm resource of B.