[Clinical application of laryngeal endoscopy in the minimally invasive surgery of laryngeal diseases].

Objective: To study the value of laryngeal endoscopic system in the treatment of laryngeal diseases with minimally invasive surgery.

Method: This paper was to summarized retrospectively the clinical experience of 34 cases suffered from laryngeal diseases treated by minimally invasive surgery with laryngeal endoscopic system. The remain tumor and hemorrhage were treated by microwave machine.

Quantitatively investigating monomethoxypolyethylene glycol modification of protein by capillary electrophoresis.

Capillary electrophoresis (CE) was applied to study quantitatively protein modification with succinimidyl succinate-activated monomethoxypolyethylene glycol (MPEG-SS). The heterogeneous distribution of modified proteins and the average modification degree were determined by CE, and the latter met with the results from 2,4,6-trinitrobenzenesulfonic acid (TNBS) spectrometric assay. It was found that the optimal buffer pH for the modification was between pH 7.

Characterization of PSII photochemistry and thermostability in salt-treated Rumex leaves.

A study was conducted, using chlorophyll fluorescence, rapid fluorescence induction kinetics, and polyphasic fluorescence transients, to determine the effect of salt treatment and heat stress on PSII photochemistry in Rumex leaves. Salt treatment was accomplished by adding NaCl solutions of different concentrations ranging from 50 to 200 mmol/L. Heat stress was induced by exposing the plant leaves to temperatures ranging from 29 to 47 degrees C.

GALA: a designed synthetic pH-responsive amphipathic peptide with applications in drug and gene delivery.

GALA is a 30 amino acid synthetic peptide with a glutamic acid-alanine-leucine-alanine (EALA) repeat that also contains a histidine and tryptophan residue as spectroscopic probes. It was designed to explore how viral fusion protein sequences interact with membranes. The sequence selected was long enough to span a bilayer in the alpha-helix, the glutamic acids (Glu) were selected to provide a pH-dependent negatively charged side-chain and the EALA repeat was adjusted so that the peptide would have a hydrophobic face of sufficient hydrophobicity to interact with the bilayer when the peptide was in an alpha-helix.

Stability of tumor necrosis factor-alpha during ion exchange chromatography.

Denaturation of target protein by various separation and purification steps contributes significant part to the total product loss in bioseparation. The conformational change and accompanying loss of activity of tumor necrosis factor-alpha during ion exchange chromatography was reversible and was decreased by adding polyethylene glycol 200 at 2 to 5% (v/v) to the eluting solution.

Preparation and characterization of PEGylated adducts of recombinant human tumor necrosis factor-alpha from Escherichia coli.

This study was conducted to get an insight into monomethoxypolyethylene glycol (MPEG) modified recombinant human TNF-alpha (rhTNF-alpha) derived from gene cloning and expression. The purification and modification processes were integrated together to make the scale-up production of PEG-modified rhTNF-alpha practical. Capillary electrophoresis was demonstrated to be a highly efficient tool in the biochemical characterization of the PEGylated products compared to slab electrophoresis.