Two distinct regulatory pathways govern Cct2-Atg8 binding in the process of solid aggrephagy.

CCT2 serves as an aggrephagy receptor that plays a crucial role in the clearance of solid aggregates, yet the underlying molecular mechanisms by which CCT2 regulates solid aggrephagy are not fully understood. Here we report that the binding of Cct2 to Atg8 is governed by two distinct regulatory mechanisms: Atg1-mediated Cct2 phosphorylation and the interaction between Cct2 and Atg11. Atg1 phosphorylates Cct2 at Ser412 and Ser470, and disruption of these phosphorylation sites impairs solid aggrephagy by hindering Cct2-Atg8 binding.

Ca2+-triggered Atg11-Bmh1/2-Snf1 complex assembly initiates autophagy upon glucose starvation.

Autophagy is essential for maintaining glucose homeostasis. However, the mechanism by which cells sense and respond to glucose starvation to induce autophagy remains incomplete. Here, we show that calcium serves as a fundamental triggering signal that connects environmental sensing to the formation of the autophagy initiation complex during glucose starvation.

Energy deprivation-induced autophagy and aggrephagy: insights from yeast and mammals.

Autophagy plays a crucial role in maintaining cellular homeostasis in response to various stimuli. Compared to research on nutrient deprivation-induced autophagy, the understanding of the molecular mechanisms and physiological/pathological significance of autophagy triggered by energy deprivation remains limited. A primary focus of our lab is to elucidate how cells sense energy deprivation and initiate autophagy.

The molecular mechanisms regulating the assembly of the autophagy initiation complex.

The autophagy initiation complex is brought about via a highly ordered and stepwise assembly process. Two crucial signaling molecules, mTORC1 and AMPK, orchestrate this assembly by phosphorylating/dephosphorylating autophagy-related proteins. Activation of Atg1 followed by recruitment of both Atg9 vesicles and the PI3K complex I to the PAS (phagophore assembly site) are particularly crucial steps in its formation.

Research and development of fully enclosed wire-shell support structure technology for deep soft rock roadway based on TRIZ theory.

The TRIZ theory was used to accurately discover the problems to be solved in the design of roadway surrounding rock control technology. This paper tried to solve the complex issue of surrounding rock control in deep roadways from a new perspective. Based on the functional component analysis and causal axis analysis of the problem's primary reason, simultaneously, the surrounding rock control technology was optimized through technical contradiction analysis, physical contradiction analysis, and substance and field model analysis.

Research on Damage Evolution Law of Glazed Hollow Beads-Cement/Sodium Silicate Grouting Materials under Different Cycles of Loading and Unloading.

With the depletion of shallow resources, deep resource mining has become a trend. However, the high temperature and complex stress environment in deep mines make resource extraction extremely challenging. This paper developed a thermal insulation grouting material made of glazed hollow beads, sodium silicate, and cement and tested the compressive strength, gelation time, and stone rate under various curing days in light of the issue of high temperature heat damage in high ground temperature mines and the impact of mining on roadway grouting bolt support.

TOR-mediated Ypt1 phosphorylation regulates autophagy initiation complex assembly.

The regulation of autophagy initiation is a key step in autophagosome biogenesis. However, our understanding of the molecular mechanisms underlying the stepwise assembly of ATG proteins during this process remains incomplete. The Rab GTPase Ypt1/Rab1 is recognized as an essential autophagy regulator.

Mec1 regulates PAS recruitment of Atg13 via direct binding with Atg13 during glucose starvation-induced autophagy.

Mec1 is a DNA damage sensor, which performs an essential role in the DNA damage response pathway and glucose starvation-induced autophagy. However, the functions of Mec1 in autophagy remain unclear. In response to glucose starvation, Mec1 forms puncta, which are recruited to mitochondria through the adaptor protein Ggc1.

Performance degradation and damage model of rice husk ash concrete under dry-wet cycles of sulfate environment.

Rice husk ash concrete (RHAC) is a new type of concrete that has been rapidly gaining acceptance in recent years. In this paper, the improvement effect of rice husk ash (RHA) on the sulfate erosion performance of concrete was confirmed. The ratio of rice husk ash concrete (RHAC) was optimized and compared with ordinary concrete (OC).

A syringe-aided apta-nanosensing method for colorimetric determination of acetamiprid.

A syringe-aided apta-nanosensing method is reported for the colorimetric determination of acetamiprid. The method employs double-stranded (ds) DNA-conjugated gold nanoparticle@magnetic agarose beads, i.e.

Effects of High Temperature on Creep Behaviour of Glazed Hollow Bead Insulation Concrete.

Glazed hollow bead insulation concrete (GHBC) presents a promising application prospect in terms of its light weight and superior fire resistance. However, only a few studies have focused on the creep behaviour of GHBC exposed to high temperatures. Therefore, in this study, the mechanism of high temperature on GHBC is analysed through a series of tests on uniaxial compression and multistage creep of GHBC, exposed from room temperature up to 800 °C.

Mitochondrial Fusion Machinery Specifically Involved in Energy Deprivation-Induced Autophagy.

Mitochondria are highly dynamic organelles, which can form a network in cells through fusion, fission, and tubulation. Its morphology is closely related to the function of mitochondria. The damaged mitochondria can be removed by mitophagy.

Performance Degradation and Microscopic Analysis of Lightweight Aggregate Concrete after Exposure to High Temperature.

This study analyses the deterioration of mechanical properties in lightweight concrete after exposure to room temperature (20 C) and high temperature, i.e., up to 1000 C, including changes in visual appearance, loss of mass, and compressive strength.

Atg11 is required for initiation of glucose starvation-induced autophagy.

How energy deprivation induces macroautophagy/autophagy is not fully understood. Here, we show that Atg11, a receptor protein for cargo recognition in selective autophagy, is required for the initiation of glucose starvation-induced autophagy. Upon glucose starvation, Atg11 facilitates the interaction between Snf1 and Atg1, thus is required for Snf1-dependent Atg1 activation.

Printing the Ultra-Long Ag Nanowires Inks onto the Flexible Textile Substrate for Stretchable Electronics.

Printing technology offers a simple and cost-effective opportunity to develop all-printed stretchable circuits and electronic devices, possibly providing ubiquitous, low-cost, and flexible devices. To successfully prepare high-aspect-ratio Ag nanowires (NWs), we used water and anhydrous ethanol as the solvent and polyvinylpyrrolidone (PVP) as the viscosity regulator to obtain a water-soluble Ag NWs conductive ink with good printability. Flexible and stretchable fabric electrodes were directly fabricated through screen printing.

An aptamer based aggregation assay for the neonicotinoid insecticide acetamiprid using fluorescent upconversion nanoparticles and DNA functionalized gold nanoparticles.

An acetamiprid-binding aptamer (ABA), gold nanoparticles (AuNPs) and upconversion nanoparticles (UCNPs) are used in a colorimetric and fluorometric method for the ultrasensitive and selective detection of the pesticide acetamiprid. The ABA is first configured into a duplex with a complementary DNA covalently attached to AuNPs. The resulting dsDNA-functionalized AuNP probe is not stable in 0.

NIR light-activated upconversion semiconductor photocatalysts.

Harvesting of near infrared (NIR) light in the abundant and environmentally friendly solar spectrum is particularly significant to enhance the utilization rate of the cleanest energy on earth. Appreciating the unique nonlinear optical properties of upconversion materials for converting low-energy incident light into high-energy radiation, they become the most promising candidates for fabricating NIR light-active photocatalytic systems by integrating with semiconductors. The present review summarizes recent NIR light-active photocatalytic systems based on a sequence of NaYF-based, fluoride-based, oxide-based and Ln ion-doped semiconductor-based photocatalysts for degradation of organic molecules.

Tunable and ultra-stable UV light-switchable fluorescent composites for information hiding and storage.

Advanced fluorescent materials have demonstrated great value in the anti-counterfeiting field for information storage and hiding. In this work, a novel strategy for producing UV light-switchable fluorescent patterns to hide and store information is proposed based on carbon dots (CDs) and Y2O3:Eu composites with exceptional optical properties. CDs can present blue emission under both 254 nm and 365 nm UV light excitation, but Y2O3:Eu can only arouse a red color under 254 nm light excitation.

Acid-degradable lactobionic acid-modified soy protein nanogels crosslinked by ortho ester linkage for efficient antitumor in vivo.

It remains a crucial challenge to achieve efficient cellular uptake and intracellular drug release in tumor cells for the nanoscale drug delivery systems. Herein, acid-degradable nanogels were prepared by cross-linking methacrylated soy protein with an acid-labile ortho ester cross-linker (NG1), and then modified with lactobionic acid (LA) to give tumor-targeted nanogels (NG2). Both NG1 and NG2 displayed excellent stability in neutral environment, while showed pH-triggered degradation behaviors under mildly acidic conditions resulting from the breakage of ortho ester bonds.

Low molecular weight polyethylenimine-grafted soybean protein gene carriers with low cytotoxicity and greatly improved transfection in vitro.

A series of gene carriers (SP-PEI) have been synthesized by acylation reaction between soybean protein and branched polyethylenimine with low molecular weight of 600, 1200 and 1800 Da, and designed as SP-PEI600, SP-PEI1200 and SP-PEI1800, respectively. SP-PEI could effectively condense plasmid DNA into nanoscale polyplexes with size range of 100-200 nm, and exhibited much lower cytotoxicity against 293T and SH-SY5Y cells than that of branched polyethylenimine (25 kDa). In vitro gene transfection demonstrated that SP-PEI/DNA complex displayed increased transfection against 293T and SH-SY5Y cells with the increase of the weight ratio of SP-PEI/DNA complex with or without 10% serum.

Preparation and RGB upconversion optic properties of transparent anti-counterfeiting films.

Advanced anti-counterfeiting labels have aroused an intensive interest in packaging industry to avoid the serious issue of counterfeit. However, the preparation and cost of the existing labels associated with the drawbacks, including the complex and high-cost equipment, limit the protection of the authenticity of goods. Herein, we developed a series of anti-counterfeiting labels based on multicolor upconversion micro-particles (UCMPs) inks via straightforward and low-cost solutions, including spin-coating, stamping and screen printing.

Facile Synthesis of Silver Nanowires with Different Aspect Ratios and Used as High-Performance Flexible Transparent Electrodes.

Silver nanowires (Ag NWs) are the promising materials to fabricate flexible transparent electrodes, aiming to replace indium tin oxide (ITO) in the next generation of flexible electronics. Herein, a feasible polyvinylpyrrolidone (PVP)-mediated polyol synthesis of Ag NWs with different aspect ratios is demonstrated and high-quality Ag NWs transparent electrodes (NTEs) are fabricated without high-temperature thermal sintering. When employing the mixture of PVP with different average molecular weight as the capping agent, the diameters of Ag NWs can be tailored and Ag NWs with different aspect ratios varying from ca.