Electron Transfer-Driven Nanozymes Boost Biosensor Sensitivity via a Synergistic Signal Amplification Strategy.

The conventional gold nanoparticles (AuNPs) with insufficient brightness face substantial challenges in developing a sensitive lateral flow immunoassay (LFIA). Herein, multibranched manganese-gold (Mn-Au) nanoparticles (MnAuNPs) with a Au core-Mn shell nanostructure were synthesized by a one-pot method. The Mn shell of valence-rich and Au core of high electron transfer efficiency endowed MnAuNPs with oxidase-like activity, which oxidized 3,3',5,5'-tetramethylbenzidine (TMB) only by electron transfer.

Multiplex immunochromatographic assay based on triple-color aggregation-induced emission fluorescent microspheres with good biocompatibility for the simultaneous detection of veterinary drugs in aquatic products.

Background: The simultaneous presence of multiple veterinary drug residues in a single sample poses a significant challenge for food safety analysis. Compared with traditional single-target detection, multiplex detection can not only provide richer sample information to raise the detection efficiency and reduce the possibility of false positives and omissions, but also decrease sample volume, shorten detection time, and lower costs. The luminance of aggregation-induced emission fluorescent microspheres (AIEFMs) can be enhanced by loading more fluorophores.

Supramolecular self-assembly of high-loaded horseradish peroxidase and biorecognized antibody into Au/polydopamine nanocomposites for sensitive immunoassay of E. coli O157:H7 in milk.

It is an urgent need of rapid and sensitive method for detection of Escherichia coli O157:H7 (E. coli O157:H7), a class of hazardous foodborne pathogens in food safety. The traditional enzyme-linked immunosorbent assay (ELISA), a dominant rapid detection technic, takes disadvantages of low test sensitivity due to the insufficient enzyme loading capacity.

Bifunctional bovine serum albumin modification driven sensitivity-enhanced lateral flow immunoassay for small molecule hazards monitoring in food.

Traditional lateral flow immunoassays (TLFIAs) are valued for their simplicity, speed, and user-friendliness. However, the specificity of conventional test strips often necessitates large quantities of antigen-protein conjugates for target detection, which can be resource-intensive. Here, we present a strategy aimed at enhancing the universality of test strips while reducing the consumption of antigen-protein conjugates, without compromising sensitivity.

Broad-spectrum detection of benzimidazoles with lateral flow immunoassay: A computational chemistry-assisted hapten design strategy and explore of molecular recognition mechanism.

Benzimidazoles (BMZs) are a class of veterinary drugs with a benzimidazole ring, the abuse of which poses a serious threat to ecological balance and human health. Consequently, the development of broad-spectrum antibodies and rapid assays are crucial for detecting BMZs in food samples. Herein, we scientifically designed three hapten structures, predicted the availability of the hapten with computational chemistry, and subsequently verified the broad-spectrum with immunological experiments.

Dual-assist heterologous antigens screening: An effective strategy to improve the sensitivity of lateral flow immunoassay.

Heterologous strategy has promising applications in improving the sensitivity of competitive immunoassay. In this study, the potential heterologous coating antigens (HEA) were screened from eight imidacloprid (IMI) structural analogs based on the cross-reactivity (CR) of a prepared antibody. Computer-aided molecular modeling was used to predict the optimal HEA.

Effects of antidiabetic agents on platelet characteristics with implications in Alzheimer's disease: Mendelian randomization and colocalization study.

Background: Observational studies have found a potential link between the use of thiazolidinediones (TZDs) and a lower risk of Alzheimer's disease (AD) development. Platelets were the great source of amyloid-β (Aβ) and involved in the development of AD. This study aimed to assess the correlation between antidiabetic agents and platelet characteristics, hoping to provide a potential mechanism of TZDs neuroprotection in AD.

Phage-based magnetic capture method as an aid for real-time recombinase polymerase amplification detection of Salmonella spp. in milk.

Salmonella is a major cause of foodborne diseases worldwide. Conventional rapid assays for detecting Salmonella in real samples often encounter severe matrix interference or detect a limited number of species of a genus, resulting in inaccurate detection. In this study, we developed a method that combined phage-based magnetic capture with real-time recombinase polymerase amplification (RPA) for the rapid, highly sensitive, and specific detection of Salmonella in milk with an ultra-low detection limit.

Novel litchi-like Au-Ag nanospheres driven dual-readout lateral flow immunoassay for sensitive detection of pyrimethanil.

Pyrimethanil (PYR) is a fungicide that is harmful to consumers when present in foods at concentrations greater than maximum permitted residue levels. High-performance immunoprobes and dual-readout strategy may be useful for constructing sensitive lateral flow immunoassay (LFIA). Herein, the prepared litchi-like Au-Ag bimetallic nanospheres (LBNPs) exhibited high mass extinction coefficients and fluorescence quenching constants.

Integration of a biocompatible metal-phenolic network and fluorescence microspheres as labels for sensitive and stable detection of carbendazim with a lateral flow immunoassay.

High fluorescence intensity microspheres such as aggregation-induced emission fluorescence microspheres (AIEFM) have improved the sensitivity of lateral flow immunoassay (LFIA). The preparation of immune probes in LFIA usually adopts the chemical coupling strategy with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide for antibody coupling, which has the problems of low coupling efficiency, tedious coupling process, and poor repeatability. A biocompatible metal-phenolic network (MPN), which contains large amounts of phenols and galloyl groups, could easily, quickly, and stably couple with antibodies.

Development of High-Performance and Multifunctional Nanoparticles Powered the Integrated Diagnosis and Treatment of .

Pathogenic diseases that trigger food safety remain a noteworthy concern due to substantial public health, economic, and social burdens worldwide. It is vital for developing an integrated diagnosis and treatment strategy for bacteria, which could achieve quick detection of pathogenic bacteria and the inhibition of multidrug-resistant bacteria. Herein, we reported an organic molecule (M-3) possessed strong light capture capacity, emerging a low energy gap and Δ.

Quercetin-induced degradation of RhoC suppresses hepatocellular carcinoma invasion and metastasis.

Background: Tumor metastasis and recurrence are major causes of mortality in patients with hepatocellular carcinoma (HCC) that is still lack of effective therapeutic targets and drugs. Previous reports implied that ras homolog family member C (RhoC) plays a toxic role on metastasis and proliferation of cancer.

Methods: In this research, the correlation between RhoC and metastasis ability was confirmed by in vitro experiments and TCGA database.

Efficient Photothermal Sensor Based on Coral-Like Hollow Gold Nanospheres for the Sensitive Detection of Sulfonamides.

Gold nanoparticles (AuNPs), universally regarded as colorimetric signal reporters, are widely employed in lateral flow immunoassays (LFIAs). However, it is difficult for AuNPs-LFIA to achieve a wide range and sensitive detection. Herein, novel coral-like hollow gold nanospheres (CHGNPs) are synthesized.

Dramatic fluorescence enhancement of PCN-224 and its application in "turn off" immunoassay for sensitive detection of E. coli O157:H7 in milk.

In this study, a type of luminescent porous coordination network-224 (PCN-224) in alkaline conditions was synthesized with the dramatic fluorescence enhancement by 20.4 times, which was explained by the fact that the decrease of Zr content in alkaline conditions resulted in the partial recovery of the electron cloud density of 4,4',4'',4'''-(Porphine-5,10,15,20-tetrayl) tetrakis(benzoic acid) (TCPP). Given the large overlap between the excitation spectrum of PCN-224 and the absorption band of Ag nanoparticles (Ag NPs), the coating of the Ag layer on PCN-224 triggered the fluorescence quenching effect, which was applied to "turn off" fluorescence immunoassay for sensitive detection of Escherichia coli O157:H7 (E.

"Four-In-One" Multifunctional Dandelion-Like Gold@platinum Nanoparticles-Driven Multimodal Lateral Flow Immunoassay.

The traditional lateral flow immunoassay (LFIA) with a single signal output mode may encounter challenges such as low sensitivity, poor detection range, and susceptibility to external interferences. These limitations hinder its ability to meet the growing demand for advanced LFIA. To address these issues, the rational development of multifunctional labels for multimodal LFIA emerges as a promising strategy.

Molecular Engineering and Confinement Effect Powered Ultrabright Nanoparticles for Improving Sensitivity of Lateral Flow Immunoassay.

The application of traditional lateral flow immunoassay (LFIA)-based gold nanoparticles (AuNPs) to measure traces of target chemicals is usually challenging. In this study, we developed an integrated strategy based on molecular engineering and the spatial confinement of nanoparticles (NPs) to obtain ultrahigh quantum yields (QYs) of aggregation-induced emission (AIE) fluorescence NPs and employed them for the highly sensitive detection of T-2 toxin on the LFIA platform. Tetraethyl-4,4',4″,4‴-(ethene-1,1,2,2-tetrayl)tetrabenzoate (TCPEME), an AIE luminogen, was designed using molecular engineering to lower the energy gap, achieving higher QYs (26.

Biomineralization-powered integrated immunoprobe and its application in Immunochromatographic assay.

Immunochromatographic assay (ICA) has attracted widespread attention owing to its advantages of economy, simplicity, and rapidity. However, the synthesis of immunoprobes is still limited by complicated design ideas and multistep operations from preparing nanoparticles to conjugating monoclonal antibodies (mAb) onto nanoparticles. Inspired by the biomineralization of zeolitic imidazolate framework-8 (ZIF-8), we proposed a strategy for the rapid synthesis of an integrated immunoprobe (ZIF-8@QDs-mAb), achieving a one-step integration with strong fluorescent signal output capability and specific recognition ability.

Sensitive fluorescence ELISA for the detection of zearalenone based on self-assembly DNA nanocomposites and copper nanoclusters.

Zearalenone (ZEN), produced by Fusarium species, is a potential risk to human health. Traditional enzyme-linked immunosorbent assay (ELISA) is restricted due to low sensitivity for the detection of ZEN. Herein, enzyme nanocomposites (ALP-SA-Bio-ssDNA, ASBD) were prepared with the self-assembly strategy based on streptavidin-labeled alkaline phosphatase (SA-ALP) and dual-biotinylated ssDNA (B-ssDNA).

High-Performance Fluorescent Microspheres Based on Fluorescence Resonance Energy Transfer Mode for Lateral Flow Immunoassays.

The label with a large Stokes shift and strong fluorescence properties could improve the sensitivity of the lateral flow immunoassay (LFIA). Herein, two aggregation-induced emission (AIE) luminogens with spectral overlap were encapsulated in polymers by using the microemulsion method as a label, and the construction of a fluorescence resonance energy transfer mode was further verified via theoretical calculation and spectral analysis. Satisfactorily, the doped AIE polymer microspheres (DAIEPMs) exhibited a large Stokes shift of 285 nm and a 10.

Molecular Engineering Powered Dual-Readout Point-of-Care Testing for Sensitive Detection of O157:H7.

O157:H7 ( O157:H7) has become one of the major threats to public health and food safety. However, the culture method as a gold standard for the detection of O157:H7 requires laborious operations and a long processing time. Herein, we developed a dual-readout aggregation-induced emission nanoparticle-based lateral flow immunoassay (LFIA) for sensitive detection of O157:H7 to achieve a qualitative and quantitative assay for satisfying the applications under varying scenarios.

Competitive ratiometric fluorescent lateral flow immunoassay based on dual emission signal for sensitive detection of chlorothalonil.

In this study, we develop a competitive ratiometric fluorescent lateral flow immunoassay (CRF-LFIA) based on dual emission fluorescence signal, which has great advantage in visual and quantitative detection of Chlorothalonil (CTN). Red-emitted fluorescent magnetic nanobeads (FMNBs) and green-emitted aggregation-induced emission fluorescent microsphere (AIEFM) are synthesized and conjugated to antibodies and antigens respectively, resulting in competitive binding with the analyte. The ratiometric fluorescence signal which comes from the overlap of these two fluorescence emissions.