Production of Monacolin K in : Comparison between Industrial Strains and Analysis of Its Gene Clusters.

strains are widely applied to yield a cholesterol synthesis inhibitor monacolin K (MK), also called lovastatin (LOV). However, the mechanism of MK production by strains is still unclear. In this study, we firstly confirmed four strains, MS-1, YDJ-1, YDJ-2, and K104061, isolated from commercial MK products as and compared their abilities to produce MK in solid-state and liquid-state cultures.

Effect of Static Magnetic Field on M7 Based on Transcriptome Analysis.

The effects of a static magnetic field (SMF) on M7 ( M7) cultured on potato dextrose agar (PDA) plates under SMF treatment at different intensities (5, 10, and 30 mT) were investigated in this paper. The results revealed that, compared with the control (CK, no SMF treatment), the SMF at all tested intensities did not significantly influence the morphological characteristics of M7, while the intracellular and extracellular pigments (MPs) and extracellular citrinin (CIT) of M7 were increased at 10 and 30 mT SMF but there was no impact on the MPs and CIT at 5 mT SMF. The transcriptome data of M7 cultured at 30 mT SMF on PDA for 3 and 7 d showed that the SMF could increase the transcriptional levels of some relative genes with the primary metabolism, including the carbohydrate metabolism, amino acid metabolism, and lipid metabolism, especially in the early growing period (3 d).

Lunasin abrogates the expression of matrix metalloproteinases and reduction of type II collagen.

Impairment of type II collagen caused by MMPs in response to overproduction of IL-1β is an important step in the pathological progression of osteoarthritis (OA). Lunasin, a well-known peptide present in the soybean, has displayed a positive impact on numerous physiological functions. Little information in the effects of lunasin on cartilage degradation has been sought in clinical research before.

The association of nucleotide-binding oligomerization domain 2 gene polymorphisms with the risk of asthma in the Chinese Han population.

Background: Genetic background is one of the important risk factors for development of asthma. The nucleotide-binding oligomerization domain 2 (NOD2) has been involved in the pathogenesis of asthma. The purpose of this study was to explore the relationship between NOD2 gene polymorphisms and asthma susceptibility in the Chinese Han population.

Calibration Method of an Ultrasonic System for Temperature Measurement.

System calibration is fundamental to the overall accuracy of the ultrasonic temperature measurement, and it is basically involved in accurately measuring the path length and the system latency of the ultrasonic system. This paper proposes a method of high accuracy system calibration. By estimating the time delay between the transmitted signal and the received signal at several different temperatures, the calibration equations are constructed, and the calibrated results are determined with the use of the least squares algorithm.

Surgical Treatment for Central Calcified Thoracic Disk Herniation: A Novel L-Shaped Osteotome.

Few reports are available on the posterior transfacet approach for the treatment of central calcified thoracic disk herniation (TDH). The objective of this study was to assess outcomes and complications in a consecutive series of patients with TDH who underwent posterior transfacet decompression and diskectomy with segmental instrumentation and fusion. The data for 27 patients (16 males and 11 females) were retrospectively reviewed and analyzed, including clinical presentation, blood loss, operative time, pre- and postoperative complications, visual analog scale, Japanese Orthopedic Association (JOA) score, and Frankel grade.