Optimal decision-making in relieving global high temperature-related disease burden by data-driven simulation.

The rapid acceleration of global warming has led to an increased burden of high temperature-related diseases (HTDs), highlighting the need for advanced evidence-based management strategies. We have developed a conceptual framework aimed at alleviating the global burden of HTDs, grounded in the One Health concept. This framework refines the impact pathway and establishes systematic data-driven models to inform the adoption of evidence-based decision-making, tailored to distinct contexts.

Theoretical evaluation of the effect of bimetallic Au-based alloy catalysts on initial N electroreduction pathways.

Theoretical studies on the effect of bimetallic Au-based alloy catalysts on initial N electroreduction pathways at the present simulated electrode/aqueous interfaces based on DFT calculations are conducted in this work. The calculated results indicate that the alloying of Au with the transition metals Ni, Pd, Pt, Ru and Ta can facilitate the activation of N molecules in the presence of the electrode/aqueous interface, which may be derived from the kinetic overpotential of the outer Helmholtz plane. The N reduction pathway may be adsorption strength-dependent on N, in which the incorporation of transition metals with a strong chemical affinity for N molecules may lead to a dissociative mechanism the initial NN bond cleavage pathway, whereas the incorporation of transition metals with medium N binding strength may make N reduction proceed by the associative mechanism the initial NH formation pathway.

Developmental validation of forensic DNA-STR kits: Expressmarker 16+10Y and expressmarker 16+18Y.

DNA-STR analysis is widely used in the forensic science field and obtaining results in shorter time is highly demanded. The developed forensic STR Kit, referred to as Expressmarker 16+10Y (EX16+10Y) and Expressmarker 16+18Y (EX16+18Y), could amplify the common autosomal and Y chromosome STR loci simultaneously. The kits are validated by a series of tests, including DNA mixtures, stutter ratios, PCR based studies, species specificities, inhibitors, sensitivity, sizing precision, reproducibility and parallel tests.

[Development of a Forensic Multiplex Amplification STR Kit for 15 Autosomal STR Loci and 10 Y-STR Loci].

Objective: To establish a multiplex STR genotyping method for autosomal STR and Y-STR loci in forensic biological practice.

Methods: Widely used autosomal STR loci and Y-STR loci were selected. A set of PCR primers was designed, and a 5-dye fluorescent labeled STR multiplex PCR reagent kit was developed.

Developmental validation of the EX20+4 system.

The EX20+4Y System is a polymerase chain reaction (PCR)-based amplification kit that enables typing of 19 autosomal short tandem repeat (STR) loci (i.e., CSF1PO, D13S317, D16S539, D18S51, D21S11, D3S1358, D5S818, D7S820, D8S1179, FGA, TH01, TPOX, vWA, Penta D, Penta E, D2S1338, D19S433, D12S391, D6S1043), four widely used Y chromosome-specific STR (Y-STR) loci (DYS458, DYS456, DYS391, DYS635), and amelogenin.

Developmental validation of a forensic rapid DNA-STR kit: Expressmarker 16.

DNA-STR analysis is widely used in the forensic science field and has important requirements on the analysis time to obtain faster inspections. The developed forensic STR kit, referred to as Expressmarker 16 (EX16), could shorten the amplification time to a minimum of 35min. It enables 16 STR loci to be co-detected, including 13 CODIS loci, D2S1338, D6S1043 and Amelogenin loci.

A guide RNA sequence design platform for the CRISPR/Cas9 system for model organism genomes.

Cas9/CRISPR has been reported to efficiently induce targeted gene disruption and homologous recombination in both prokaryotic and eukaryotic cells. Thus, we developed a Guide RNA Sequence Design Platform for the Cas9/CRISPR silencing system for model organisms. The platform is easy to use for gRNA design with input query sequences.

[Forensic application of expressmarker 22 STR loci direct PCR amplification kit].

Objective: To explore the application value of Expressmarker 22 STR loci direct PCR amplification kit.

Methods: One thousand nine hundred and forty-eight samples (including samples spotted on FTA cards, filter papers and case samples) were tested using Expressmarker 22 STR loci direct PCR amplification kit. At the same time, all were tested using Sinofiler kit, Identifiler kit and PowerPlex 16 kit respectively for comparison.

[Rapid DNA identification using 6+1 STR kit and EX-Q20 electrophoresis].

Objective: To establish a rapid STR genotyping method for individual identification.

Methods: Two hundred blood samples from FTA were collected. Equal amount of blood were collected by puncher and analyzed using two methods (6+1 STR kit in combination with EX-Q20 electrophoresis and Sinofiler kit in combination with POP4 electrophoresis).

[Treatment of 58 cases of vitiligo by electro-plum-blossom needle therapy combined with catgut implantation at acupoints under TDP radiation].

Objective: To search for a method for increasing therapeutic effect on vitiligo.

Methods: One hundred and sixteen cases were randomly divided into a treatment group and a control group, 58 cases in each group. The treatment group were treated with electro-plum-blossom needle therapy plus catgut implantation at local acupoints under TDP radiation, and the control group with external application of sicorten.

[Combinative effects of FAP-1 antisense oligonucleotide and carboplatin on apoptosis of ovarian cancer cell SKOV3].

Background & Objective: Recent studies have shown that overexpression of Fas associated phosphatase-1 (FAP-1) can been detected in human ovarian cancer cell line SKOV3, suggesting that this overexpression may play an important role in the tumorigenesis and drug resistance of ovarian cancer. This study was designed to explore the effects of fas associated phosphatase-1 antisense oligonucleotide (FAP-1 ASODN) combined with carboplatin on the apoptosis of ovarian cancer cell SKOV3.

Methods: Antisense oligonucleotide technique was used to transfect FAP-1 ASODN into SKOV3 cells.

[The manufacture of pelvic mirror physical model using rapid prototyping].

Objective: To study the manufacture of pelvic mirror physical model using rapid prototyping and the accuracy of the models.

Methods: The pelvic CT images were acquired by spiral CT thin slice scanning, and the slicing data of pelvis were created from these CT images by computer image-processing. Then the pelvic mirror physical model was manufactured using rapid prototyping.