Boric Acid-Fueled ATP Synthesis by F F ATP Synthase Reconstituted in a Supramolecular Architecture.

Significant strides toward producing biochemical fuels have been achieved by mimicking natural oxidative and photosynthetic phosphorylation. Here, different from these strategies, we explore boric acid as a fuel for tuneable synthesis of energy-storing molecules in a cell-like supramolecular architecture. Specifically, a proton locked in boric acid is released in a modulated fashion by the choice of polyols.

pH responsive ATP carriers to drive kinesin movement.

Multilayer film coated CaCO3 microspheres were employed as pH responsive ATP carriers to drive kinesin movement. The production of oxygen scavengers in a kinesin-microtubule system induces the decomposition of ATP-loaded CaCO3 microspheres and then leads to the release of ATP.

Transporting a tube in a tube.

LbL-assembled tubes were employed for micro/nanoscale cargo transportation through the kinesin-microtubule system. Selectively modified with kinesins onto the inner tube walls through Ni-NTA complexes, these tubes can work as channels for microtubules. A motility assay shows the smooth movement of microtubules along the tube inner wall powered by the inside immobilized kinesins.

A self-powered kinesin-microtubule system for smart cargo delivery.

A smart self-powered cargo delivery system that is composed of creatine phosphate kinase (CPK) microspheres, kinesins and microtubules is demonstrated. The CPK microsphere not only acts as an ATP generation and buffering system, but also as a carrier for cargo transport, thus realizing the easy loading and self-powered delivery of cargos at the same time.