Expression of a LINE-1 endonuclease variant in gastric cancer: its association with clinicopathological parameters.

Background: Long interspersed nuclear element-1 (LINE-1 or L1), the most abundant and only autonomously active family of non-LTR retrotransposons in the human genome, expressed not only in the germ lines but also in somatic tissues. It contributes to genetic instability, aging, and age-related diseases, such as cancer. Our previous study identified in human gastric adenocarcinoma an upregulated transcript GCRG213, which shared 88% homology with human L1 sequence and contained a putative conserved apurinic/apyrimidinic endonucleas1 domain.

[Preparation and identification of monoclonal antibody against human GCRG213].

Aim: To prepare and characterize the monoclonal antibody against human GCRG213.

Methods: The HIS-GCRG213 fusion protein was expressed in E.coli.

Tissue microarray analysis of topoisomerase IIalpha protein in gastric adenocarcinomas: histogenetic and prognostic implications.

Unlabelled: The aim of this study was to: To investigate topoisomerase IIα (topo-IIα) expression and its correlation with clinicopathological parameters in primary gastric cancer patients.

Patients And Methods: A tissue microarray including tumor, paired non-tumoral and lymph node metastasis specimens from 210 gastric adenocarcinoma patients was built for immunohistochemical interrogation. The correlation between topo-IIα expression and patient clinicopathological parameters was evaluated by univariate and multivariate analyses.

[Preparation, identification and preliminary application of the polyclonal antibody against gastric cancer-related protein GCRG224].

Aim: To prepare the polyclonal antibody against gastric cancer-related protein GCRG224.

Methods: The thioredoxin/GCRG224 fusion protein was expressed in E.coli.

[Expression of S100A6 in primary and metastatic human gastric cancer].

Objective: Some members of the S100 gene family have been suggested to be associated with cancer development and metastasis. Our previous cDNA micro-array studies have showed S100A6 expression is elevated in gastric cancer compared with that in paired normal mucosa. To validate our previous results and further investigate the possible role of S100A6 gene in gastric cancer, we carried out this detailed S100A6 expression analysis in more matched gastric cancer samples.

LINE-1 family member GCRG123 gene is up-regulated in human gastric signet-ring cell carcinoma.

Aim: To analyze the expression profiles of a human gastric-cancer-related gene, GCRG123, in human gastric signet-ring cell carcinoma tissues, and to perform bioinformatics analysis on GCRG123.

Methods: In situ hybridization was used to explore the GCRG123 expression pattern in paraffin-embedded gastric tissues, including 15 cases of signet-ring cell carcinoma, 15 of intestinal-type adenocarcinoma, and 15 of normal gastric mucosa. Northern blotting was used to analyze the differences in GCRG123 expression between stomach signet-ring cell carcinoma and intestinal-type adenocarcinoma tissues.

[Construction of a gastric cancer related gene GCRG213-specific siRNA expression vector and its effect on gastric cancer cells in vitro].

Objective: To investigate the effect of gene GCRG213 siRNA transfection into gastric cancer cell line MKN45 cells.

Methods: Two pairs of DNA sequences containing small hairpin structure to GCRG213 were designed and synthesized. The complement form was obtained by annealing and inserted into RNAi expression vector IMG-800.

[Preparation and characterization of rabbit antibody against gastric cancer-related protein GCRG213].

Aim: To prepare the rabbit antibody against gastric cancer-related protein GCRG213.

Methods: The thioredoxin/GCRG213 fusion protein was expressed in E. coli.

[Gene expression profile of human adenocarcinoma by cDNA microarray and clustering].

Objective: To investigate the gene expression profile of human gastric adenocarcinoma by means of cDNA microarray and to analyze its biological significance.

Methods: Paired tumor and non-tumor specimens from 18 cases of advanced gastric adenocarcinoma were studied. Total RNA was isolated and labeled by reverse transcription reaction with cy5 and cy3 for cDNA probe.

Detection of K-ras gene mutation in fecal samples from elderly large intestinal cancer patients and its diagnostic significance.

Aim: To study the diagnostic significance of K-ras gene mutations in fecal samples from elderly patients with large intestinal cancer.

Methods: DNA was extracted in the fecal and tissue samples from 23 large intestinal cancer patients, 20 colonic adenomatoid polypus patients and 20 healthy subjects. The K-ras gene mutations at the first and second bases of codon 12 were detected by the allele specific mismatch method.

[Influence of dendritic cell infiltration on prognosis and biologic characteristics of progressing gastric cancer].

Objective: To study the relation between dendritic cell (DC) infiltration and clinicopathologic parameters, biologic characteristics and prognosis of progressing gastric cancer.

Methods: The development of apoptotic cell death (apoptotic index, AI) in 61 progressing gastric carcinoma tissues was analyzed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick end labeling (TUNEL) method. The PCNA labeling index (PCNA-LI), density of dendritic cells in the tumor were detected by immunohistochemical method by the LSAB kit using antibody against S-100 protein and PC-10.

A gene encoding an apurinic/apyrimidinic endonuclease-like protein is up-regulated in human gastric cancer.

Aim: To identify the gene that may predispose to human gastric cancer and to analyze its expression in gastric cancer and non-tumorous gastric mucosa.

Methods: Cancer, para-tumor, and non-tumor gastric tissues were studied for gene expression profile using fluorescent differential display reverse transcription polymerase chain reaction (DDRT-PCR). The differentially expressed bands of interest were analyzed by cloning, Northern blotting, and sequencing.

[A lamin-like protein gene is down-regulated in human gastric cancer].

Objective: To clone human gastric cancer related gene and to analyze its expression profile in gastric mucosal tissues.

Methods: Paired tumor, paratumor and non-tumor specimens from 7 gastric adenocarcinoma patients (male 4, female 3, with average age 51 +/- 18 years) were studied by means of fluorescent differential display reverse transcription polymerase chain reaction (DDRT-PCR). The differentially expressed cDNA bands of interest were cloned and analyzed by Northern blot and in situ hybridization.

[Relationship between Helicobacter pylori infection and proliferation and apoptosis of gastric epithelial dysplasia cell].

Background & Objective: Gastric epithelial dysplasia is the precancerous lesion of gastric cancer. However, the mechanism that dysplasia evolves to malignancy is not clear. In order to clarify the relationship between Helicobacter pylori (HP) infection and its virulence factor and changes of cell kinetics of dysplasia, the authors measured the changes of proliferation and apoptosis and the status of HP infection.

[Expression of human anti-apoptotic gene survivin and its splice in normal human gastric tissue and gastric cancer].

Objective: To analyze the expression of human anti-apoptotic gene survivin (SVV) in normal human gastric tissues and gastric cancer.

Methods: SVV cDNA clone was obtained from human gastric cancer tissues by virtue of RT-PCR, using Dig-marked cRNA probe in situ hybridization to analyze its expression in normal human gastric tissues and gastric cancer.

Results: Two SVV cDNA clones, SVV-S4A and SVV-S1B were obtained.

[Cloning and tissue expression analysis of up-regulated cDNA fragment in human gastric cancer].

Objective: To identify novel human gastric cancer-associated susceptibility gene for early diagnosis and treatment of gastric cancer.

Methods: A primer was designed for 3'-rapid amplification of cDNA end(RACE) and amplified fragments were cloned, then they were analyzed by sequencing. Compared with ESTs in Genbank, the EST fragment represented a novel gene.

[Co-expression of immunoglobulin light chain kappa and lambda in gastric carcinoma cell].

Objective: To study the expression of immunoglobulin light chain kappa and lambda (Igkappa and Iglambda) in gastric carcinoma cell and their co-expression.

Methods: Igkappa and Iglambda of 22 human gastric carcinoma specimens embedded in paraffin were monitored through immunohistochemical method-LSAB method.

Results: Among 22 gastric carcinoma specimens, both Igkappa and Iglambda were positive in 17 (77.