MicroRNA‑29‑3p regulates the β‑catenin pathway by targeting IGF1 to inhibit the proliferation of prolactinoma cells.

The present study aimed to analyze the effects and underlying mechanisms of microRNA (miR)‑29‑3p on the proliferation and secretory abilities of prolactinoma cells by targeting insulin‑like growth factor (IGF)‑1/β‑catenin. The relationship between miR‑29a‑3p and the survival of prolactinoma cells was analyzed with the Kaplan‑Meier method in reference to The Cancer Genome Atlas. The expression levels of miR‑29a‑3p and IGF‑1 in MMQ and GH3 cells were detected.

The Therapeutic Effect of Shugan Xiehuo Formula in the Female Rat Model with Central Precocious Puberty.

Central precocious puberty (CPP) severely affects children's physical and mental health and needs to be treated promptly and effectively. This article aimed to research the therapeutic effect of Shugan Xiehuo Formula (SXF) on CPP. A female CPP rat model was established and then treated with leuprolide and different doses of SXF.

Neo-5,22-Cholestadienol Derivatives from Karsch and Targeted Bactericidal Action Mechanisms.

The discovery and search for new antimicrobial molecules from insects and animals that live in polluted environments is a very important step in the scientific search for solutions to the current problem of antibiotic resistance. Previously, we have reported that the secondary metabolite with the antibacterial action discovered in scorpion. The current study further isolated three new compounds from karsch, while compounds and possessed 5,22-cholestadienol derivatives whose structure demonstrated broad spectrum bactericide activities.

Gallnuts: A Potential Treasure in Anticancer Drug Discovery.

In the discovery of more potent and selective anticancer drugs, the research continually expands and explores new bioactive metabolites coming from different natural sources. Gallnuts are a group of very special natural products formed through parasitic interaction between plants and insects. Though it has been traditionally used as a source of drugs for the treatment of cancerous diseases in traditional and folk medicinal systems through centuries, the anticancer properties of gallnuts are barely systematically reviewed.

Insight into the C-F bond mechanism of molecular analogs for antibacterial drug design.

The activities of biological molecules usually rely on both of intra-molecular and intermolecular interactions between their function groups. These interactions include interonic attraction theory, Van der Waal's forces and the function of geometry on the individual molecules, whether they are naturally or synthetic. The purpose of this study was to evaluate the antibacterial activity of C-F bond compound using combination of experiments verification and theoretical calculation.

From Traditional Usage to Pharmacological Evidence: A Systematic Mini-Review of Spina Gleditsiae.

Spina Gleditsiae is an important herb with various medicinal properties in traditional and folk medicinal systems of East Asian countries. In China through the centuries, it has been traditionally used as a source of drugs for anticancer, detoxication, detumescence, apocenosis, and antiparasites effects. Recently, an increasing number of studies have been reported regarding its chemical constituents and pharmacological activities.

Novel skeleton compound Allomyrinanoid A and two purine alkaloids from the adult of Allomyrina dichotoma L.

Three new compounds were isolated from the adult insect of Allomyrina dichotoma L. for the first time. A new skeleton compound is named as Allomyrinanoid A (1) originated from the familiar norbornane derivatives and two new compounds of purine alkaloid are named as adenine-9-methylaldehyde oxime B (2) and 6-N-methyleneimine-adenine-9-methylaldehyde oxime B (3).

Resveratrol trimers from seed cake of Paeonia rockii.

In the course of screening natural products for antibacterial activities, a total acetone extract of the seed cake of Paeonia rockii showed significant effects against bacterial strains. Bioactivity-guided fractionation of the EtOAc-soluble fraction of the total acetone extract resulted in the isolation and identification of five resveratrol trimers, including rockiiol C (1), gnetin H (2), suffruticosol A (3), suffruticosol B (4) and suffruticosol C (5). The relative configuration of these compounds was elucidated mainly by comprehensive 1D and 2D-NMR experiments.

Isolation and structural elucidation of novel antimicrobial compounds from maggots of Chrysomyis megacephala Fabricius.

The excretions/secretions from the maggot of Chrysomyis megacephala Fabricius are traditionally used to treat serious infections in China. In this study, bioassay-guided fractionation led to the isolation of three novel antibacterial compounds (1-3), including important fluorinated compounds (3 and 5), together with other nine known compounds from 70% methanol extract of C. megacephala.

TiO2 nanotube sensor for online chemical oxygen demand determination in conjunction with flow injection technique.

A simple, rapid and environmental friendly online chemical oxygen demand (COD) analytical method based on TiO2 nanotube sensor in conjunction with the flow injection technique was proposed to determine the COD of aqueous samples, especially for refractory organics, low-concentration wastewater, and surface water. The new method can overcome the drawbacks of the conventional COD determination methods. The results show that with the new method, each analysis takes only about 1 to 3 min, the linear range is up to 1 to 500 mg x L(-1) of the compound of interest, and the detection limit is 1 mg x L(-1).

Two bioactive compounds from the Chinese scorpion Buthusmartensii Karsch.

Two compounds, 3β-acetoxyl,2,14,22-trihydroxy,19-hydroxymethyl,9α,5β,14β-card-20(22)enolide (1) and 1,2,3,4-tetrahydro-6-hydroxy1-5-pyrimidinecarbox-aldehyde (2), were isolated from arthropods in scorpion, Buthus martensii Karsch, by medium pressure liquid chromatography with silica gel as stationary phase and RP-HPLC analysis technology. The structures were elucidated on the basis of NMR spectroscopic analysis and HR-ESI-MS determination. It was found that (1) is a novel compound and both compounds showed inhibitory activities against the Gram-positive bacteria Bacillus subtilis (minimum inhibitory concentrations = 15 μg/mL), with diameter of inhibition zone at ϕ = 6.

2-Bromo-1,6,6-trimethyl-6,7,8,9-tetra-hydro-phenanthro[1,2-b]furan-10,11-dione.

In the title compound, C19H17BrO3, the ring skeleton is located on a crystallographic mirror plane; two C atoms of the cyclo-hexene ring are disordered over the two locations to satisfy the preferred ring conformation. In the crystal, C-H⋯O hydrogen bonds link the mol-ecules into chains along the a axis. π-π stacking inter-actions between benzo-quinone rings, with a centroid-centroid distance of 3.

A theoretical investigation of the interaction between small Pd particles and 1-butyl-3-methyl imidazolium ionic liquids with Cl-, BF4(-) and PF6(-) anions.

Density functional calculations have been used to investigate the interaction between Pd(n) clusters (n = 1-6) and 1-butyl-3-methylimidazolium (Bmim(+)) based ionic liquids (ILs) with the anions [Cl(-)], [BF(4)(-)] and [PF(6)(-)]. The interaction of small Pd(n) clusters (1 ≤ n ≤ 6) with a single cation or anion is also studied. The interaction strengths in anion-Pd(n) categories with n = 1-6 follow the trend [Cl(-)] > [BF(4)(-)] > [PF(6)(-)].

Isolation and identification of antibacterial neo-compounds from the red ants of ChangBai Mountain, Tetramorium sp.

Three novel coumarin compounds along with two known amide alkaloids were isolated from a methanol extract of the red ants of ChangBai Mountain, Tetramorium sp. Their structures were identified on the basis of IR, 2D NMR ((1)H-(1)H COSY, HSQC, HMBC and NOESY) and HRESIMS analysis. Antibacterial activity of all the compounds was evaluated using KB paper diffusion through measurement of inhibiting zone.

Secondary metabolites from the roots of Phlomis umbrosa.

The phytochemical study of the roots of Phlomis umbrosa Turcz afforded a new phenylethanoid glycoside, 3-hydroxy-4-methoxy-β-phenylethoxy-O-[2,3-diacetyl-α-l-rhamnopyranosyl-(1 → 3)]-4-O-cis-feruloyl-[β-d-apiofuranosyl-(1 → 6)]-β-d-glucopyranoside (1), and two new 28-noroleanane-derived spirocyclic triterpenoids, phlomishexaol C (2) and phlomishexaol D (3). Their structures were elucidated on the basis of 1D and 2D NMR analyses, in combination with high-resolution MS experiment.

[Studies on the chemical constituents of the flowers of Bupleurum chinese].

Objective: To study the chemical constituents of the flowers of Bupleurum chinese.

Methods: The compounds were separated by column chromatography with silica gel. Their structures were identified by spectral methods and chemical analysis.

Density functional study of hydrogen binding on gold and silver-gold clusters.

A theoretical study was carried out on the binding of hydrogen on small bimetallic Ag(m)Au(n) (m + n < or = 5) and pure Au(n) (n < or = 5) clusters with neutral, negative, and positive charge state. It is found that the composition and charge state of clusters have strong influence on the most favorable binding site. The adiabatic ionization potentials, electron affinities, and hydrogen binding energies of cluster hydrides increase with the Au content increasing for the given cluster size.

[Chemical constituents from roots of Phlomis umbrosa].

Objective: To study the chemical constituents of Phlomis umbrosa.

Method: Chemical constituents were isolated by repeated column chromatography (Toyopearl HW-40C and preparative HPLC). The structures were elucidated on the basis of spectral data analysis.

Phenylethanoid glycosides from the roots of Phlomis umbrosa.

The phytochemical study of the roots of Phlomis umbrosa Turcz. afforded three new phenylethanoid glycosides, 3'''-acetyl-O-betonyoside D (1), 2''', 3'''-di-acetyl-O- betonyoside D (2), and 3''', 4'''-di-acetyl-O-betonyoside D (3), along with five known phenylethanoid glycosides. Their structures were elucidated on the basis of spectroscopic data.

A density functional study of the interaction of NCO with small copper clusters.

Density functional calculations have been carried out for Cu(n)NCO(-), Cu(n)NCO, Cu(n)NCO(+), and Cu(n)NCO(2+) clusters. It was found that for small n, charge state has a strong influence on the NCO location site. The ground states of the neutral and anionic complex clusters have a dominantly planar structure while most of the cationic complex clusters prefer three-dimensional structures.

A novel oligosaccharide ester from Syringa pubescens.

Pubescenside A (1), a novel oligosaccharide ester, has been isolated from the flowers of Syringa pubescens, together with five known compounds D-mannitol (2), meso-inositol (3), hydrostytosol (4), glucose (5), and sucrose (6). The structure of 1 was elucidated as 1-O-[beta-D-glucopyranosyl-(1-6)-beta-D-galactopyranosyl-(1-1)-beta-D-galactopyranosyl-6]-4,4-dimethylpelargonicate by chemical and spectroscopic means. The water extract of the flowers and leaves of S.

[Inhibitory effect on male mouse procreation and chemical composition analysis of Rhynchosia volubilis ethyl acetate extract].

Objective: To study the effect of Rhynchosia volubilis Lour ethyl acetate extract (RVLEAE) on male mouse procreation and analyse their chemical composition.

Method: With the method of solvent extraction, RVLEAE was extracted and concentrated. In the experiment of mice, 80 male mice were randomly and equally divided into four groups: Normal Saline control, positive control with 0.