Prognostic utility of gamma-glutamyl transpeptidase to platelet ratio in patients with solitary hepatitis B virus-related hepatocellular carcinoma after hepatectomy.

Background: The prognostic impact of preoperative gamma-glutamyl transpeptidase to platelet ratio (GPR) levels in patients with solitary hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) following radical resection has not been established.

Aim: To examine the clinical utility of GPR for prognosis prediction in solitary HBV-related HCC patients.

Methods: A total of 1167 solitary HBV-related HCC patients were retrospectively analyzed.

Clinical Significance and Potential Mechanisms of ATP Binding Cassette Subfamily C Genes in Hepatocellular Carcinoma.

The purpose of this investigation was to assess the diagnostic and prognostic significance of genes in hepatocellular carcinoma (HCC). The Student t-test was used to compare the expression level of between HCC and paraneoplastic tissues. Receiver operating characteristic curve (ROC) analysis was applied for diagnostic efficiency assessment.

Clinical implications and molecular mechanisms of Cyclin-dependent kinases 4 for patients with hepatocellular carcinoma.

Background: Hepatocellular carcinoma (HCC) was frequently considered as a kind of malignant tumor with a poor prognosis. Cyclin-dependent kinases (CDK) 4 was considered to be cell-cycle-related CDK gene. In this study, we explored the clinical significance of CDK4 in HCC patients.

CYP2C8 Suppress Proliferation, Migration, Invasion and Sorafenib Resistance of Hepatocellular Carcinoma via PI3K/Akt/p27 Axis.

Background: Cytochrome P450 2C8 (CYP2C8) gene is one of the members of the cytochrome P450 enzymes (CYPs) gene family. The aim of this study was to reveal the function of CYP2C8 in hepatocellular carcinoma (HCC) and its effect on the sorafenib resistance.

Methods: Differential expression analysis in multiple HCC datasets all suggested that CYP2C8 expression was significantly decreased in HCC tissues, compared with para-carcinoma liver tissues.

Iizukines C-E from a saline soil fungus Aspergillus iizukae.

Three new alkaloids, iizukines C-E (1-3), including two aspochalasins (1 and 2), and one brasiliamide derivative (3), along with two known aspochalasins, rosellichalasin (4) and cytochalasin Z17 (5), were isolated from the culture of Aspergillus iizukae. Compound 1 was the first aspochalasin uniquely featuring a 1,2,4-triazole functionality, and 3 showed a pair of NMR signals in CDCl with a ratio of about 2:1 due to the existence of conformational isomers. Their structures were determined by extensive spectroscopic analyses and single-crystal X-ray diffractions.

New Butenolides and Cyclopentenones from Saline Soil-Derived Fungus .

Three new -hydroxyl butenolides (-), a pair of new enantiomeric spiro-butenolides ( and ), a pair of enantiomeric cyclopentenones ( new and new natural), and six known compounds (-), were isolated from . Their structures were established by spectroscopic data and electronic circular dichroism (ECD) spectra. Two pairs of enantiomers [(+)/(-)- and (+)/(-)-] obtained from the reaction of with acetyl chloride (AcCl) confirmed that was a mixture of two pairs of enantiomers.

Potential Antiviral Xanthones from a Coastal Saline Soil Fungus .

Five new (⁻) and two known xanthones ( and ), one of the latter () obtained for the first time as a natural product, together with three known anthraquinones, questin, penipurdin A, and questinol, were isolated from the coastal saline soil-derived by application of an OSMAC (one strain many compounds) approach. Their structures were determined by interpretation of nuclear magnetic resonance (NMR) and high-resolution electrospray ionization mass spectroscopy (HRESIMS) data, as well as comparison of these data with those of related known compounds. Antiviral activity of xanthones - was evaluated through the cytopathic effect (CPE) inhibition assay, and compound exhibited distinctly strong activity towards influenza virus (H1N1), herpes simplex virus types 1 (HSV-1) and 2 (HSV-2) with IC values of 44.

Raistrickiones A-E from a Highly Productive Strain of Generated through Thermo Change.

Three new diastereomers of polyketides (PKs), raistrickiones A−C (⁻), together with two new analogues, raistrickiones D and E ( and ), were isolated from a highly productive strain of , which was subjected to an experimental thermo-change strategy to tap its potential of producing new secondary metabolites. Metabolites and existed in a diastereomeric mixture in the crystal packing according to the X-ray data, and were laboriously separated by semi-preparative HPLC on a chiral column. The structures of ⁻ were determined on the basis of the detailed analyses of the spectroscopic data (UV, IR, HRESIMS, 1D, and 2D NMR), single-crystal X-ray diffractions, and comparison of the experimental and calculated electronic circular dichroism spectra.

Pyran Rings Containing Polyketides from Penicillium raistrickii.

Five new pyran rings containing polyketides, penicipyrans A-E (-), together with the known pestapyrone A (), were isolated from the saline soil-derived . Their structures were determined by interpretation of NMR and HRESIMS data. The absolute configurations of compounds and were established by the modified Mosher's method and single-crystal X-ray diffraction analysis, respectively.

Systemic delivery of siRNA by T7 peptide modified core-shell nanoparticles for targeted therapy of breast cancer.

Systemic delivery of siRNA is the most challenging step to transfer RNAi to clinical application for breast cancer therapy. In this study, the tumor targeted, T7 peptide modified core-shell nanoparticles (named as T7-LPC/siRNA NPs) were constructed to achieve effective systemic delivery of siRNA. The core-shell structure of T7-LPC/siRNA NPs enables them to encapsulate siRNA in the core and protect it from RNase degradation during circulation.

[Secondary Metabolites from Penicillium raistrickii].

Objective: To investigate the secondary metabolites from Penicillium raistrickii.

Methods: Compounds were isolated and purified by normal and reverse phase silica gel, Sephadex LH-20 gel column chromatography and RP-HPLC. Their structures were established by means of spectral techniques and physicochemical properties.

Peniciketals A-C, new spiroketals from saline soil derived Penicillium raistrichii.

Peniciketals A-C (1-3), three new spiroketals with a benzo-fused 2,8-dioxabicyclo[3.3.1]nonane moiety, were isolated from the saline soil derived fungus Penicillium raistrichii.

Cytokinins can act as suppressors of nitric oxide in Arabidopsis.

Maintaining nitric oxide (NO) homeostasis is essential for normal plant physiological processes. However, very little is known about the mechanisms of NO modulation in plants. Here, we report a unique mechanism for the catabolism of NO based on the reaction with the plant hormone cytokinin.

Disarrangement of actin filaments and Ca²⁺ gradient by CdCl₂ alters cell wall construction in Arabidopsis thaliana root hairs by inhibiting vesicular trafficking.

Cadmium (Cd), one of the most toxic heavy metals, inhibits many cellular and physiological processes in plants. Here, the involvement of cytoplasmic Ca²⁺ gradient and actin filaments (AFs) in vesicular trafficking, cell wall deposition and tip growth was investigated during root (hair) development of Arabidopsis thaliana in response to CdCl₂ treatment. Seed germination and root elongation were prevented in a dose- and time-dependent manner by CdCl₂ treatment.

[Pharmacokinetics and relative bioavailability of ziprasidone tablets in Chinese healthy volunteers].

Objective: To investigate the pharmacokinetics and bioavailability of ziprasidone tablets in Chinese healthy volunteers.

Methods: A randomized crossover study was performed in 20 healthy volunteers, who received a single oral dose (40 mg) of the test or reference preparation of ziprasidone. Blood samples were collected from the subjects at different time points following the drug administration, and the plasma concentration of ziprasidone was determined using high-performance liquid chromatography.

Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy.

The moss Physcomitrella patens has been shown to tolerate abiotic stresses, including salinity, cold, and desiccation. To better understand this plant's mechanism of desiccation tolerance, we have applied cellular and proteomic analyses. Gametophores were desiccated over 1 month to 10% of their original fresh weight.

[Pharmacokinetics of paroxetine tablet in healthy Chinese volunteers].

Objective: To study the pharmacokinetics of paroxetine tablet in Chinese healthy volunteers.

Methods: Twenty healthy subjects received a single oral dose of 40 mg paroxetine tablet. The plasma concentrations of paroxetine were determined using high-performance liquid chromatography (HPLC) and the measurements were analyzed with 3P97 program.

The involvement of NtFtsZ2-1 gene in the regulation of chloroplast division and expansion in tobacco.

Chloroplasts are a vital group of organelles of plants, yet the molecular mechanisms associated with their division remain poorly understood. Recent studies have revealed that the FtsZ protein, known as a key component in prokaryotic cell division, is involved in chloroplast division process. The NtFtsZ2-1 gene was isolated from Nicotiana tabacum by RT-PCR, and the sense and antisense expression plasmids were used to examine the function of NtFtsZ2-1 gene in transgenic tobacco.

[Reversed phase high-performance liquid chromatography for determination of plasma olanzapine and pharmacokinetics of olanzapine in healthy Chinese volunteers].

Objective: To establish a reversed phase high-performance liquid chromatography (HPLC) system for determining plasma concentration of olanzapine and analyze the pharmacokinetics of olanzapine in healthy Chinese volunteers.

Methods: Ten healthy male subjects received a single oral dose of 20 mg olanzapine tablets. The plasma concentrations of olanzapine were determined by HPLC, and the data were analyzed using 3P97 program.

[Studies on the chemical constituents of a fungus producing perylenequinones].

Aim: To study the chemical constituents in the mycelia of Hypomyces sp..

Methods: Silica gel column chromatography was employed for the isolation and purification.

Gas-Phase and Solution-Phase Homolytic Bond Dissociation Energies of H-N(+) Bonds in the Conjugate Acids of Nitrogen Bases.

The oxidation potentials of 19 nitrogen bases (abbreviated as B: six primary amines, five secondary amines, two tertiary amines, three anilines, pyridine, quinuclidine, and 1,4-diazabicyclo[2,2,2]octane), i.e., E(ox)(B) values in dimethyl sulfoxide (DMSO) and/or acetonitrile (AN), have been measured.