Expression of DNMTs and MBD2 in GIST.

The aim of this study was to investigate the protein expression of DNA methyltransferases (DNMTs, including DNMT1, DNMT2, DNMT3A, DNMT3B and DNMT3L) and methyl-CpG-binding domain protein 2 (MBD2) in gastrointestinal stromal tumor (GIST). Immunohistochemistry and western blot analysis were used to detect expression of DNMT and MBD2 in 15 pairs of adult GIST and matched non-tumor tissues. The protein expression of DNMT1, DNMT2, DNMT3B, DNMT3L and MBD2 was significantly higher in adult GISTs compared to the matched non-tumor tissues (P<0.

[Silencing hypoxia inducible factor-2α gene by small interference RNA inhibits the growth of mammosphere cells in nude mice under hypoxic microenvironment].

Objective: To explore the effects of silencing hypoxia inducible factor-2α (HIF-2α) by small interference RNA on the growth of mammosphere cells in nude mice under hypoxic microenvironment.

Methods: The empty and interference vectors were transfected into MCF-7 cell. Then G418 was added to screen the positive cells to obtain stable cell line.

Expression of DNMTs and genomic DNA methylation in gastric signet ring cell carcinoma.

The aim of the present study was to investigate the protein expression of DNA methyltransferases (DNMTs) and genomic DNA methylation status of genomes in gastric signet ring cell carcinoma (SRC). Immunohistochemistry was performed to analyze DNMT expression and methylated DNA immunoprecipitation microarray (MeDIP‑chip) and MeDIP quantitative real‑time PCR (MeDIP‑qPCR) were performed to analyze the genomic DNA methylation status in gastric SRC tissue. An increase in DNMT1 and decrease in DNMT3A expression in SRC tissue was observed compared with matched non‑cancerous tissue.

[Expression of breast cancer resistance protein and p-glycoprotein in residual breast cancer tissue after chemotherapy and its correlation with cancer stem cells].

Objective: To compare the expression differences of breast cancer resistance protein(BCRP/ABCG2) and P-glycoprotein(P-gp) in breast cancer tissue before chemotherapy and in residual breast cancer tissue, and to explore its correlation with breast cancer stem cells.

Methods: Immunohistochemistry was used to detect the expression of ABCG2, P-gp, and breast cancer stem cells(BCSCs) markers(CD44 and CD24) in breast cancer tissue before chemotherapy and residual breast cancer tissue after chemotherapy. Immunofluorescence was applied for determination of the CD44 and CD24 protein expressions of BCSCs microspheres cells.

Integrin-linked kinase functions as a tumor promoter in bladder transitional cell carcinoma.

The aim of this study was to elucidate the role of the integrin-linked kinase (ILK) gene in development of human bladder transitional cell carcinoma (BTCC). Expression of ILK protein and ILK mRNA in 56 cases of human BTCC tissue and in 30 cases of adjacent normal bladder tissue was detected by immunohistochemistry S-P and reverse transcription polymerase chain reaction (RT-PCR), respectively. Four specific miRNA RNAi vectors targeting human ILK were synthesized and transfected into BIU-87 cells by liposome to obtain stable expression cell strains.

[Inhibitory effect of pyrrolidine dithiocarbamate combined with matrine on the growth of human hepatocellular carcinoma xenografts].

Objective: To investigate the relationship between activation of nuclear factor-K-gene binding (NF-κB) and apoptosis induced by matrine(MT) in transplanted tumor of human hepatocellular carcinoma in nude mouse.

Methods: Tumors were established by injection of hepatocellular carcinoma cell line HepG2 into the back of nude mice. The mice were divided randomly into four groups: Control group, MT group (35 mg/kg), PDTC group (120 mg/kg) and Combination group: PDTC + MT group (120 mg/kg + 35 mg/kg), the reagents were injected peritoneally.

Overexpression of Forkhead Box M1 transcription factor and nuclear factor-κB in laryngeal squamous cell carcinoma: a potential indicator for poor prognosis.

The Forkhead Box M1 transcription factor and nuclear factor-κB have been shown to play important roles in the development and progression of human cancers. However, the functional significance of Forkhead Box M1 transcription factor in laryngeal squamous cell carcinoma and the correlation between Forkhead Box M1 transcription factor and nuclear factor-κB remain unclear. In the current study, we have shown that Forkhead Box M1 transcription factor and nuclear factor-κB were significantly overexpressed in laryngeal squamous cell carcinoma tissues and precancerous lesions, compared with adjacent normal tissues (both P < .

[Expression of paxillin in breast cancer cell with high and low metastatic potentiality].

Objective: To study the expression of paxillin in the two subgroups of human breast cancer cells with high and low metastatic potentialities and the effect of paxillin on tumor metastasis and adhesion.

Methods: Human breast cancer MDA-MB-435s cells were cultured in Transwell chamber with artificial matrigel, two subgroups of MDA-MB-435s cells with different metastatic potentiality were obtained by the ability of cells penetrating artificial matrigel. The expressions of paxillin mRNA and protein were examined by Immune histochemistry, Western blot and RT-PCR.

The role of Crk/Dock180/Rac1 pathway in the malignant behavior of human ovarian cancer cell SKOV3.

Small GTPases, particularly the Rho family, are key regulators of cell motility and migration. Dock180 was well known for the main target of signal adaptor protein Crk and acted as a guanine-nucleotide exchange factor for small GTPase Rac1. In the present study, Dock180 was found to combine primarily with CrkI other than CrkII, and its association with Elmo1 was also demonstrated in ovarian cancer cell SKOV3.

[Effect of epigallocatechin-3-gallate on the invasiveness of hepatocarcinoma cells in vitro].

Objective: The effect of EGCG on the invasion of HepG2 cells in vitro was studied.

Methods: The expressions of MUC1 in HepG2 cells before and after they were treated with EGCG were studied with immunohistochemistry. The effects of EGCG on the invasion of HepG2 cells were evaluated using Transwell chambers attached with polycarbonate filters and reconstituted basement membranes (Matrigel).

[Inhibition of NF-kappa B activity enhanced apoptosis induced by matrine in hepatocellular carcinoma cells].

Objective: To investigate the relationship between activation of nuclear factor-kappa gene binding (NF-kappaB) and apoptosis induced by matrine in hepatocellular carcinoma cell line HepG2.

Methods: HepG2 cells were stimulated by different concentrations of matrine (0.8, 1.

[Separation and identification of the exosomes derived from a mouse hepatoma carcinoma cell line (H22) and initial investigation of their protein composition].

Objective: To separate and identify the exosomes derived from a mouse hepatoma carcinoma cell line (H22) and to detect their protein composition, and to investigate the possibility of using these exosomes as a kind of tumor vaccine.

Methods: Exosomes were purified by serial ultracentrifugation and sugar density ultracentrifugation, and then they were observed and identified by electron microscopy. Exosomes underwent peptide mass fingerprint and Western blot analyses.

[Effects of TNBG on the proliferation and apoptosis of human hepatocellular carcinoma cell line QGY-7701].

Objective: To investigate the effects and mechanism of TNBG on the proliferation and apoptosis of human hepatocellular carcinoma cell line QGY-7701.

Methods: The 3H-TdR incorporation and MTT assay were used to test the effects of anti-proliferation and cytotoxicity respectively, the morphological changes of the cancer cells were examined under light and electron microscopes. The Flow cytometric analysis was performed to detect the cell cycle distribution and apoptosis.

[Study on the angiogenic ability of tumor fibroblasts].

Objectives: To explore the role of fibroblasts derived from tumor in the tumor angiogenesis.

Methods: Two-well co-culture system were used to detect the expression of MMP-9, TGF-beta1, TN and bcl-2 in L929-H22 cells, and their ability of promoting angiogenesis of ECV304 cells and invasion of MDA-MB-231 cells respectively, which were established in our laboratory before. Then their adhesion and the effect of their supernatant on H22 cells proliferation were analysed.

[Establishment of a cisplatin-induced human hepatocellular carcinoma drug-resistant cell line and its biological characteristics].

Background & Objective: Drug resistance is a popular topic in tumor study. The drug-resistant cell line established in vitro is generally used as the research model. To explore the mechanism of resistance of hepatocellular carcinoma(HCC) to cisplatin, we designed this study to establish a cisplatin-induced human hepatocellular carcinoma drug-resistant cell line and study its characteristics.

Interaction between cisplatin, 5-fluorouracil and vincristine on human hepatoma cell line (7721).

AIM:To evaluate the killing effects of CDDP, 5-Fu and VCR on human hepaoma cell line (7721).METHODS:The median-effect principle was used.RESULTS:Killing effects of the individual drug were enhanced as the median concentration increased.