Spatial Transcriptomics Analysis Reveals that CCL17 and CCL22 are Robust Indicators of a Suppressive Immune Environment in Angioimmunoblastic T Cell Lymphoma (AITL).

Background: T cell lymphoma is a complex and highly aggressive clinicopathological entity with a poor outcome. The angioimmunoblastic T-cell lymphoma (AITL) tumor immune microenvironment is poorly investigated.

Methods: Here, to the best of our knowledge, spatial transcriptomics was applied for the first time to study AITL.

A computational method for large-scale identification of esophageal cancer-related genes.

The incidence of esophageal cancer has obvious genetic susceptibility. Identifying esophageal cancer-related genes plays a huge role in the prevention and treatment of esophageal cancer. Through various sequencing methods, researchers have found only a small number of genes associated with esophageal cancer.

Wilms' Tumor and Benign Renal Tumor Combined With Hypospadias and Incomplete Orchiocatabasis Appearing Simultaneously in a 10 Months Old Boy.

We herein report a case of left renal Wilms' tumor and right renal hamartoma combined with hypospadias and incomplete orchiocatabasis in a 10-month-old boy. In the literature to date, no case has been reported. The preoperative abdominal computerized tomography (CT) scan was suggestive of bilateral nephroblastomas, and clinical diagnosis was bilateral renal tumors with external genitals malformation, a syndrome? Finally, this case was used by B-ultrasonic guided percutaneous biopsy to help determine the nature of bilateral renal tumors.

Simvastatin Attenuates Neurogenetic Damage and Improves Neurocongnitive Deficits Induced by Isoflurane in Neonatal Rats.

Background/aims: Isoflurane inhibited neurogenesis and induced subsequent neurocognitive deficits in developing brain. Simvastatin exerts neuroprotection in a wide range of brain injury models. In the present study, we investigated whether simvastatin could attenuate neurogenetic inhibition and cognitive deficits induced by isoflurane exposure in neonatal rats.

Multidisciplinary effort in treating children with hepatoblastoma in China.

The purpose of this study is to report the first nationwide protocol (Wuhan Protocol) developed by Chinese Children's Cancer Group and the results of multidisciplinary effort in treating hepatoblastoma. In this study, we reported the final analysis, which includes 153 hepatoblastoma patients in 13 hospitals from January 2006 to December 2013. The 6-year overall survival and event-free survival rates were 83.

[Anatomical structures of Coleus forskohlii transplanted in Tongcheng].

Objective: To investigate the vegetative tissues of Coleus forskohlii cultivated in Tongcheng, Hubei Province, and to provide useful information for its planting.

Methods: The root, stem, leaf and enlarged rhizome of Coleus forskohlii were subject to routine paraffin section and staining with safranin and fast green FCF solution before examination by light microscopy.

Results: The secondary tissue was well developed in root, and stem showed a higher percentage of cortex and pitch, and 4 large vascular bundles.

[Culture and identification of neural stem cells from mouse embryos].

Objective: The purpose of this study was to culture and identify neural stem cells from mouse embryos in vitro using a modified method and provide a basis for further study of the biology of neural stem cells under hypoxia.

Methods: The cells were isolated mechanically from the front cortex of fetal Institute of Cancer Research (ICR) mice on embryonic day 14. They were passaged by mechanical dissociation and enzymatic digestion.

The role of NGX6 and its deletion mutants in the proliferation, adhesion and migration of nasopharyngeal carcinoma 5-8F cells.

Objective: To demonstrate the subcellular localization of nasopharyngeal carcinoma (NPC)-associated gene 6 (NGX6) and its basic structure and function.

Methods: The deletion mutants of NGX6 were constructed by one-step PCR and transfected into NPC cell line 5-8F. The subcellular location of NGX6 or its mutants was detected by immunofluorescence staining of cytoplasm (CYTO) and nuclear protein, and immunoelectron-microscopic analysis.

[Preparation of anti-LRRC4 polyclonal antibody and its application in constructing expression profile of human gliomas with different pathological grades].

Objective: To prepare anti-LRRC4 polyclonal antibody and analyze the correlation between the expression of LRRC4 and pathological grades of gliomas in rabbits.

Methods: Appropriate protein sequence with good hydrophilicity and antigenicity was chosen by analyzing with DS Gene 1.1 software.