Clin Hemorheol Microcirc
July 2024
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
February 2011
Aim: To analyze the effect of recombinant IL-1β on proliferation, migration, and the effect on IFNα induced cell growth inhibtion.
Methods: The vector pLIVE-mIL-1β was transfected into Hepa1-6 cells mediated by transIT-LT1. Gene expression level of IL-1β was analyzed by RT-PCR and Sandwich ELISA.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
December 2007
Aim: To investigate the effect of the expression of recombinant IL-1beta in H22 hepatoma cells on its response to NK cell mediated cytotoxicity.
Methods: BALB/c mouse was stimulated by 6% of starch. Total RNA was prepared from peripheral blood monocytes (PBMCs).
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
August 2007
Aim: To investigate the inhibitory effect of IL-1beta antisense RNA on the sensitivity of HepG2 cells to the NK cell mediated cytotoxicity.
Methods: Two gene segments of IL-1beta [IL-1beta1(17-331, 315 bp) and IL-1beta2(246-505, 260 bp)] were selected for antisense RNA. Total RNA was extracted from PBMC of a healthy donor treated with LPS.