A pathogenesis-related protein 1 of Cucurbita moschata responds to powdery mildew infection.

Pumpkin ( Duch.) productivity is severely hindered by powdery mildew (PM) worldwide. The causative agent of pumpkin PM is , a biotrophic fungus.

Corrigendum: Expression of Pumpkin Gene Improves Powdery Mildew Resistance in Tobacco.

[This corrects the article DOI: 10.3389/fpls.2020.

Participates in the Defense Response of Pepper to through Regulating the Expression of Defense-Related Genes.

Squamosa promoter binding protein (SBP)-box genes are plant-specific transcription factors involved in plant growth and development, morphogenesis and biotic and abiotic stress responses. However, these genes have been understudied in pepper, especially with respect to defense responses to infection. is a SBP-box family gene in pepper that was identified in our previous research.

Expression of Pumpkin Gene Improves Powdery Mildew Resistance in Tobacco.

Powdery mildew (PM), caused by , is a major threat to the global cucurbit yield. The molecular mechanisms underlying the PM resistance of pumpkin ( Duch.) are largely unknown.

Improved Powdery Mildew Resistance of Transgenic Overexpressing the Gene.

Powdery mildew (PM), which is mainly caused by , is a serious biotrophic pathogen disease affecting field-grown and greenhouse-grown cucurbit crops worldwide. Because fungicides poorly control PM, the development and cultivation of PM-resistant varieties is critical. A homolog of (), which encodes a key component of the plant disease-associated signal transduction pathway, was previously identified through a transcriptomic analysis of a PM-resistant pumpkin () inbred line infected with PM.

Identifying Key Genes of Liver Cancer by Networking of Multiple Data Sets.

Liver cancer is one of the deadliest cancers in the world. To find effective therapies for this cancer, it is indispensable to identify key genes, which may play critical roles in the incidence of the liver cancer. To identify key genes of the liver cancer with high accuracy, we integrated multiple microarray gene expression data sets to compute common differentially expressed genes, which will result more accurate than those from individual data set.

Transcriptome profiling of pumpkin (Cucurbita moschata Duch.) leaves infected with powdery mildew.

Cucurbit powdery mildew (PM) is one of the most severe fungal diseases, but the molecular mechanisms underlying PM resistance remain largely unknown, especially in pumpkin (Cucurbita moschata Duch.). The goal of this study was to identify gene expression differences in PM-treated plants (harvested at 24 h and 48 h after inoculation) and untreated (control) plants of inbred line "112-2" using RNA sequencing (RNA-Seq).

An efficient method to transcription factor binding sites imputation via simultaneous completion of multiple matrices with positional consistency.

Transcription factors (TFs) are DNA-binding proteins that have a central role in regulating gene expression. Identification of DNA-binding sites of TFs is a key task in understanding transcriptional regulation, cellular processes and disease. Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) enables genome-wide identification of in vivo TF binding sites.

Nonconvex Penalty Based Low-Rank Representation and Sparse Regression for eQTL Mapping.

This paper addresses the problem of accounting for confounding factors and expression quantitative trait loci (eQTL) mapping in the study of SNP-gene associations. The existing convex penalty based algorithm has limited capacity to keep main information of matrix in the process of reducing matrix rank. We present an algorithm, which use nonconvex penalty based low-rank representation to account for confounding factors and make use of sparse regression for eQTL mapping (NCLRS).

Different conjugated system Zn(ii) Schiff base complexes: supramolecular structure, luminescent properties, and applications in the PMMA-doped hybrid materials.

A series of Zn(ii) complexes with different conjugated systems, [ZnL1Cl] (Zn1), [ZnL2Cl] (Zn2), [Zn(L3)]·(ClO) (Zn3), [ZnL4Cl] (Zn4), and [ZnL5Cl] (Zn5), were synthesized and subsequently characterized via single crystal X-ray diffraction, H and C NMR, FT-IR, elemental analyses, melting point, and PXRD. The X-ray diffraction analyses revealed that the supramolecular frameworks of complexes Zn1-Zn5 are constructed by C-HO/Cl hydrogen bonds and ππ interactions. Complexes Zn1-Zn3 feature 3D 6-connected {4·6} topological structures, whereas complex Zn4 exhibits a 3D 7-connected supramolecular framework with a {4·6} topological structure.

Collaborative Completion of Transcription Factor Binding Profiles via Local Sensitive Unified Embedding.

Although the newly available ChIP-seq data provides immense opportunities for comparative study of regulatory activities across different biological conditions, due to cost, time or sample material availability, it is not always possible for researchers to obtain binding profiles for every protein in every sample of interest, which considerably limits the power of integrative studies. Recently, by leveraging related information from measured data, Ernst et al. proposed ChromImpute for predicting additional ChIP-seq and other types of datasets, it is demonstrated that the imputed signal tracks accurately approximate the experimentally measured signals, and thereby could potentially enhance the power of integrative analysis.

HONHCl optimized CHNHPbI films for improving performance of planar heterojunction perovskite solar cells via a one-step route.

Planar heterojunction perovskite solar cells (PHJ-PSCs) constructed with one-step precursor solution spin-coating deposition (OPSSD) usually give an extremely low performance mainly due to the poor morphology and low crystallinity of the perovskite films. In this work, by incorporating a suitable HONHCl additive in the perovskite precursor solution, a high quality perovskite film with improved morphology and crystallinity was obtained. The UV-vis measurement of the CHNHI solutions without and with HONHCl demonstrates that the improved quality of the perovskite film can be easily attributed to a combined effect of N, I, HO and CHNHCl originating from the oxidation of CHNHI triggered by the HONHCl additive, which can manipulate the crystallization process of the perovskite.

Thermally-induced single-crystal-to-single-crystal transformations from a 2D two-fold interpenetrating square lattice layer to a 3D four-fold interpenetrating diamond framework and its application in dye-sensitized solar cells.

In this work, a rare 2D → 3D single-crystal-to-single-crystal transformation (SCSC) is observed in metal-organic coordination complexes, which is triggered by thermal treatment. The 2D two-fold interpenetrating square lattice layer [Cd(IBA)2]n (1) is irreversibly converted into a 3D four-fold interpenetrating diamond framework {[Cd(IBA)2(H2O)]·2.5H2O}n (2) (HIBA = 4-(1H-imidazol-1-yl)benzoic acid).

ChIP-PIT: Enhancing the Analysis of ChIP-Seq Data Using Convex-Relaxed Pair-Wise Interaction Tensor Decomposition.

In recent years, thanks to the efforts of individual scientists and research consortiums, a huge amount of chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) experimental data have been accumulated. Instead of investigating them independently, several recent studies have convincingly demonstrated that a wealth of scientific insights can be gained by integrative analysis of these ChIP-seq data. However, when used for the purpose of integrative analysis, a serious drawback of current ChIP-seq technique is that it is still expensive and time-consuming to generate ChIP-seq datasets of high standard.

Advanced Cd(II) complexes as high efficiency co-sensitizers for enhanced dye-sensitized solar cell performance.

This work reports on two new complexes with the general formula [Cd3(IBA)3(Cl)2(HCOO)(H2O)]n (1) and {[Cd1.5(IBA)3(H2O)6]·3.5H2O}n (2), which can be synthesized by the reaction of Cd(II) with rigid linear ligand 4-HIBA containing imidazolyl and carboxylate functional groups [4-HIBA = 4-(1H-imidazol-1-yl)benzoic acid].

Tunable Luminescence and Application in Dye-Sensitized Solar Cells of Zn(II)/Hg(II) Complexes: Methyl Substitution-Induced Supramolecular Structures Based on (E)-N-(6-Methoxypyridin-2-ylmethylene)arylamine Derivatives.

Using Schiff-base ligands (E)-N-(6-methoxypyridin-2-yl)(CH═NAr) (where Ar = C6H5, L1; 2-MeC6H4, L2; 2,4,6-Me3C6H2, L3), six Zn(II)/Hg(II) complexes, namely, [ZnL1Cl2] (Zn1), [HgL1Cl2] (Hg1), [ZnL2Cl2] (Zn2), [HgL2Cl2] (Hg2), [ZnL3Cl2] (Zn3), and [HgL3Cl2] (Hg3) have been synthesized under solvothermal conditions. The structures of six complexes have been established by X-ray single-crystal analysis and further physically characterized by EA, FT-IR, (1)H NMR, and ESI-MS. The crystal structures of these complexes indicate that noncovalent interactions, such as hydrogen bonds, C-H···Cl, and π···π stacking, play essential roles in constructing the resulting supramolecular structures (1D for Hg3; 2D for Zn2, Hg2; 3D for Zn1, Hg1, and Zn3).

Solvatochromic and application in dye-sensitized solar cells of sandwich-like Cd(II) complexes: supramolecular architectures based on N(1),N(3)-bis[(6-methoxypyridin-2-yl)methylene]benzene-1,3-diamine.

A novel polydentate Schiff base ligand N(1),N(3)-bis[(6-methoxypyridin-2-yl)methylene]benzene-1,3-diamine (L) and its two dinuclear sandwich-like complexes {[CdL(NO3)(H2O)]·NO3}2 (1) and {[CdL(CH3CN)(H2O)]·(ClO4)2·(CH3CN)2}2 (2) were synthesized. Both C-H∙∙∙O, C-H∙∙∙N and π-π non-covalent interactions had essential roles in constructing the resulting three-dimensional supramolecular networks. L emits a more intense blue-green fluorescence emission around 493 nm than in dilute solution, exhibiting stacking-induced emission properties.

Synthesis and characterization of substituted Schiff-base ligands and their d(10) metal complexes: structure-induced luminescence tuning behaviors and applications in co-sensitized solar cells.

Nine IIB group complexes, [ZnL1Cl2] (Zn1), [CdL1Cl2]2 (Cd1), [HgL1Cl2] (Hg1), [ZnL2Cl2] (Zn2), [CdL2Cl2] (Cd2), [HgL2Cl2] (Hg2), [ZnL3Cl2] (Zn3), [CdL3Cl2] (Cd3) and [HgL3Cl2] (Hg3), have been synthesized from the corresponding ortho-(6-methoxy-pyridyl)(CH[double bond, length as m-dash]NAr) (where Ar = 2,6-iPr2C6H3, L1; 4-MeC6H4, L2; 2-OMeC6H4, L3) Schiff base and structurally characterized by elemental analysis, FT-IR, (1)H NMR and X-ray single-crystal analysis. Crystallographic studies reveal that the center metal of the complexes adopts a distorted tetrahedron geometry (except for Cd1 and Cd3, which display square pyramidal geometry) and C-HCl hydrogen bonds and ππ stacking interactions contribute to three-dimensional supramolecular structures. The series of complexes exhibit tunable luminescence from blue, through green, to light yellow by varying the temperature (298 K and 77 K), both in solution and in the solid state.

Reduced tolerance to abiotic stress in transgenic Arabidopsis overexpressing a Capsicum annuum multiprotein bridging factor 1.

Background: The pepper fruit is the second most consumed vegetable worldwide. However, low temperature affects the vegetative development and reproduction of the pepper, resulting in economic losses. To identify cold-related genes regulated by abscisic acid (ABA) in pepper seedlings, cDNA representational difference analysis was previously performed using a suppression subtractive hybridization method.

[Detection of antibiotics in water using silver colloid films as substrate of surface-enhanced Raman scattering].

In the present paper, silver colloid films prepared by self-assembly method were used as surface-enhanced Raman scattering (SERS) active substrates to achieve trace detection of antibiotics in water. Silver colloids were prepared using the microwave heating method, and silver colloids films were prepared with self-assembly method. The enhancement of sliver colloid films to antibiotics was analysed by changing the pH value of silver colloid and times of films.

Suppression Subtractive Hybridization Analysis of Genes Regulated by Application of Exogenous Abscisic Acid in Pepper Plant (Capsicum annuum L.) Leaves under Chilling Stress.

Low temperature is one of the major factors limiting pepper (Capsicum annuum L.) production during winter and early spring in non-tropical regions. Application of exogenous abscisic acid (ABA) effectively alleviates the symptoms of chilling injury, such as wilting and formation of necrotic lesions on pepper leaves; however, the underlying molecular mechanism is not understood.

[Effect of necrostatin-1 on apoptosis induced by aluminum and its mechanism].

Objective: To study the effect of necrostatin (Nec-1) on apoptosis induced by aluminum (Al), and approach the mechanism.

Methods: Neural cell death model was made by 4 mmol/L Al treated neuroblastoma cells (SH-SY5Y). Cell viabilities were detected at different concentrations of Al and/or Nec-1.

Simple method of 'on-plate' growth for improved antibody screening.

Antibody phage display is a powerful biomolecular selection technology now routinely used for refining antibody diversity towards analytes of both therapeutic and diagnostic interest. Post selection, automated robotic systems can be utilised to pick, express and analyse large numbers of putative analyte-specific clones allowing the parallel screening of thousands of antibodies in less time. Most screening techniques involve a spatial addressing process whereby the selected antibodies are extracted from the cells and analysed to verify specificity.