[Simultaneous Determination of Four Bioactive Components in Chrysanthemi Flos by Quantitative Analysis of Multi-Components by Single Marker].

Objective: To establish a method of simultaneous determination of chlorogenic acid,galuteolin,quercetin and acacetin in Chrysanthemi Flos by quantitative analysis of multi-components by single marker( QAMS),and determine the application value of QAMS in the quality control of Chrysanthemi Flos.

Methods: The analysis was established to use the Shim-pack C18( 250 mm × 4. 6 mm,5 μm),the mobile phase of water contained acetonitrile( A)-0.

DNMT1 activates the canonical Wnt signaling in rheumatoid arthritis model rats via a crucial functional crosstalk between miR-152 and the DNMT1, MeCP2.

In previous study, we identified that microRNA (miR)-152 expression was down-regulated in RA model rats, and overexpression of miR-152 inhibited the canonical Wnt signaling through the DNA methyltransferase (DNMT1) inhibition. However, the exact molecular mechanisms of DNMT1 were unclear. In this work, we investigate whether DNMT1 affects the pathogenesis of RA model rats and targets the miR-152 promoter.

MicroRNA-663 activates the canonical Wnt signaling through the adenomatous polyposis coli suppression.

Rheumatoid arthritis (RA) is a symmetrical polyarticular autoimmune disease of unknown etiology. In this present study, we observed that the adenomatous polyposis coli (APC) expression is down-regulated and the expression of microRNA (miR)-663 increased significantly in synovium from RA patients compared with control. Target gene prediction for miR-663 revealed that the mRNA of APC gene, which is a member of the canonical Wnt signaling pathway, has a miR-663 binding site in its 3'-untranslated region (3'UTR).

miR-375 regulates the canonical Wnt pathway through FZD8 silencing in arthritis synovial fibroblasts.

Whether the rheumatoid arthritis (RA) pathogenesis is regulated by microRNA (miRNA) is not entirely clear. In this study, we found that miR-375 was down-regulated significantly in fibroblast-like synoviocytes (FLS) in adjuvant-induced arthritis (AIA) rat model compared with control. Because the web-based software TargetScan and PicTar predict Frizzled 8 (FZD8) as the target of miR-375, we investigated whether up-regulated miR-375 plays a role in the activation of the canonical Wnt signaling by targeting the FZD8.

Giardia intestinalis: effects of Pulsatilla chinensis extracts on trophozoites.

Pulsatilla chinensis is a medicinal root plant that has been used to treat a wide range of disease conditions. Our study determined the antiprotozoal activity of various P. chinensis extracts and fractions against Giardia intestinalis including their effects on parasite growth, cell viability, adherence, and morphology.

[Study on tissue culture of pasqueflower].

Objective: To speed up seedling production of pasqueflower (Puzlsatilla chinenses) and their modernization in pasqueflower.

Method: With tissue culture method, primary culture of different explants, culture of cluster buds and their rooting culture were conducted on medium of treatment combinations of adding different hormones.

Result: The appropriate medium for different culture stages were MS + 6-BA 1.