[Chemical Constituents from Semiliquidambar cathayensis Roots].

Objective: To study the chemical constituents from the roots of Semiliquidambar cathayensis.

Methods: The roots of Semiliquidambar cathayensis were extracted with 80% ethanol for reflux. Chemical constituents were isolated by silica gel chromatography and Sephadex LH-20 column chromatography from petrol ether part and ethyl acetate part of extracts.

[Study on Chemical Constituents of Stems from Altingia chinensis].

Objective: To study the chemical constituents of the stems from Altingia chinensis.

Methods: The stems from Altingia chinensis were extracted with 95% ethanol for reflux,and the extract were evaporated. The chemical constituents were isolated by silica gel chromatography and Sephadex LH-20 column chromatography from petrol ether part and ethyl acetate part of extract.

Preoperative platelet/lymphocyte ratio is a superior prognostic factor compared to other systemic inflammatory response markers in ovarian cancer patients.

The aim of the present study was to determine the most meaningful preoperative prognostic factor of cancer-related death in ovarian cancer patients by comparing potentially prognostic systemic inflammatory response (SIR) markers. The levels of fibrinogen, albumin, C-reactive protein (CRP), and serum cancer antigen-125 (CA-125) and the neutrophil/lymphocyte ratio (NLR) and platelet/lymphocyte ratio (PLR) were evaluated in 190 ovarian cancer patients to identify predictors of overall survival (OS) and progression-free survival (PFS) using univariate and multivariate analyses. Patients with a PLR >203 had a shorter PFS and OS than the patients in PLR ≤203 group (11 vs.

[Proteinchip-flight mass spectrometry for screening differentially expressed proteins in the serum of cervical cancer patients].

Objective: To identify the differentially expressed proteins in the serum of patients with cervical cancer for use as the biomarkers for early diagnosis of cervical cancer.

Methods: Surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) with weak cationic chips (CM10) was used to examine the serum samples of 24 patients with cervical squamous cell carcinoma and 25 age-matched healthy women. The protein fingerprints were obtained, and bioinformatic analysis was performed to identify the differentially expressed proteins in the serum of the patients.

[Differentially expressed genes between upward and downward progressing types of nasopharyngeal carcinoma].

Background & Objective: Upward (local growth and invasion of the base of skull), downward (distant metastasis) and mixed progressing types of nasopharygeal carcinoma (NPC) have been observed when the disease progress to middle-late stage. The upward and downward progressing types are evidently different in clinical symptom, therapy strategy and prognosis. Identification of the molecular differences between them is very important for molecular classification, prognostic prediction and research on neoplasia and development of NPC.

[Topical dimethyl sulfoxide and intralesional hyaluronidase administration for vinorelbine extravasation-induced rat skin injury].

Objective: To evaluate the effects of topical DMSO and intralesional hyaluronidase administration, used alone or in combination, on skin injury due to vinorelbine extravasation in rats.

Methods: Skin injury due to vinorelbine extravasation was induced in the lower extremities of 30 SD rats, which were treated subsequently with topical DMSO, intralesional hyaluronidase, their combination, topical saline, and intralesional saline, with the rats without any treatment as the control. The wound area on 1, 4, 8, 12, 18, 24, 30 days and the time of healing were observed and compared.

[Garlic oil inhibits cyclin E expression in gastric adenocarcinoma cells].

Objective: To explore the inhibitory effect of garlic oil on cyclin E expression in gastric adenocarcinoma cells.

Methods: Human gastric adenocarcinoma SGC7901 cells were cultured routinely and the expressions of transforming growth factor alpha (TGFalpha) and epidermal growth factor receptor (EGFR) are detected by immunofluorescent staining and flow cytometry. The SGC7901 cells were also cultured with RPMI 1640 without calf serum for 48 h, followed by further culture with RPMI 1640 in the presence of 2.

[Efficacy of chitosan and hyaluronidase on skin damage caused by docetaxel extravasation in rats].

Background & Objective: Docetaxel extravasating into the surrounding tissues may lead to severe skin damage. No guideline for handling docetaxel extravasation has been proposed till now. This study was to explore the efficacy of chitosan and hyaluronidase on skin damage caused by docetaxel extravasation in a rat model.

Therapeutic targeting of the endothelin a receptor in human nasopharyngeal carcinoma.

The endothelin A receptor (ET(A)R) autocrine pathway is overexpressed in many malignancies, including nasopharyngeal carcinoma (NPC). In this tumor, ET(A)R expression is an independent determinant of survival and a robust independent predictor of distant metastasis. To evaluate whether ET(A)R represents a new target in NPC treatment, we tested the therapeutic role of ET(A)R in NPC.

[Effect of transforming growth factor alpha on the expression of cyclin E and cyclin D1 in gastric carcinoma cells].

Objective: To explore the effect of transforming growth factor alpha (TGFalpha) on the expression of cyclin E and D1 in gastric carcinoma cells.

Methods: Human gastric adenocarcinoma SGC7901 cells were cultured routinely and synchronized at G(0)/G(1) phase in serum-free RPMI-1640. The percentage of the cells at G(0)/G(1) phase was detected by propidium iodide staining and flow cytometry (FCM), and the synchronized cells were cultured in RPMI-1640 supplemented with 2.

[Proteome analysis for identification of tumor-associated biomarkers in breast cancer].

Background & Objective: Pre-symptomatic screening of early-stage breast cancer may greatly reduce tumor-related mortality. Some tumor markers, such as CA15-3 and CA27-29, are recommended only for monitoring therapy of advanced or relapsed breast cancer. This study was to find new biomarkers that could be used individually or in combination with an existing modality for cost-effective screening of breast cancer by proteome analysis.

New serum biomarkers for detection of HBV-induced liver cirrhosis using SELDI protein chip technology.

Aim: To find new serum biomarkers for liver cirrhosis (LC) in chronic carriers of hepatitis B virus (HBV).

Methods: Surface enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry was used to discover biomarkers for differentiating HBV induced LC from non-cirrhotic cohorts. A training population of 25 patients with HBV-induced LC, 20 patients with HCC, and 25 closely age-matched healthy men, was studied.

[Association of TGFalpha and cyclin E expression in gastric carcinoma and precancerous lesions].

Background & Objective: Previous studies have indicated that increased expression of TGFalpha and cyclin E are correlated with the oncogenesis and progress of cancer; however, their expression patterns in gastric precancerous lesions have been not clear yet. In addition, the association between expression of TGFalpha and cyclin E has not been reported. This study was designed to investigate the expression of TGFalpha and cyclin E in chronic superficial gastritis (CSG), gastric precancerous lesions, and gastric carcinoma (GCA), and analyze the association of TGFalpha and cyclin E expression, and the relationship between their expression and different development stages of oncogenesis of GCA.

[Cloning of NP9 gene and its influence on cyclin D1 transcription activity].

Background & Objective: We cloned NP9 gene (GenBank, BF718797) in a previous study which was down-regulated in nasopharyngeal carcinoma (NPC). To clarify the function of NP9 gene, we cloned the coding sequence (CDS) of NP9 gene and investigated the influence of NP9 on cyclin D1 expression.

Methods: A full-length cDNA sequence was obtained by Blast NP9 EST, then the complete CDS was cloned into an eukaryotic expressing vector pRc/CMV2.