[Establishment of a Mouse Model of Acquired Aplastic Anemia Mediated by Cyclophosphamide Combined with Cyclosporine].

Objective: To establish a stable mouse model of acquired aplastic anemia.

Methods: Female BALB/C mice aged 6 months were intraperitoneally injected with cyclophosphamide and cyclosporine for 14 days. The number of peripheral blood cells, the concentration of hemoglobin, the number of bone marrow nucleated cells, bone marrow smear, bone marrow pathological sections and other indexes were observed.

[Effects of partial root-zone irrigation and rational close planting on yield and water productivity of cotton in arid area.].

The objective of this study was to evaluate the effects and underlying physiological mecha-nisms of partial root zone irrigation (PRI) and rational close planting, as well as their interaction on yield and water productivity (WP) of cotton and to explore new alternatives of water-saving irrigation in dry land areas. A factorial field experiment with irrigation mode (normal irrigation, partial root-zone irrigation and deficient irrigation) and plant population density (135000, 180000 and 225000 plants·hm) was conducted in the west of Inner Mongolia to examine their effects on cotton growth, yield, water productivity and related physiological characters. The results showed that the irrigation mode and plant density as well as their interaction significantly affected the biomass, yield, yield components and harvest index.

A new effective process for production of curdlan oligosaccharides based on alkali-neutralization treatment and acid hydrolysis of curdlan particles in water suspension.

Biologically active β-1,3-oligosaccharides with rapidly growing biomedical applications are produced from hydrolysis of curdlan polysaccharide. The water-insoluble curdlan impedes its hydrolysis efficiency which is enhanced by our newly developed alkali-neutralization treatment process to increase the stability of curdlan suspension to more than 20 days, while the untreated control settled within 5 min. A putative double-layer structure model comprising of a compact core and a hydrated outer layer was proposed to describe the treated curdlan particles based on sedimentation and scanning electron microscopy observation.

[Effects of warming-reinforcing acupuncture combined with rehabilitation training on the early motor function of hemiparalysis patients caused by ischemic brain stroke: a randomized and controlled study].

Objective: To verify the improvement function of warming-reinforcing acupuncture combined with rehabilitation training on the early motor function of hemiparalysis patients caused by ischemic brain stroke.

Methods: Eighty cases were randomly divided into a warming-reinforcing acupuncture combined with rehabilitation training group (group A) and a rehabilitation training group (group B), 40 cases in each group. Both groups were treated with internal routine treatment.

[Analysis on heavy metal concentrations in agricultural soils of Baoshan, Shanghai].

Based on the acquisition of heavy metal data from 216 topsoil samples of the agricultural land in Baoshan District, a typical region of Shanghai City, the content, distribution characteristics and sources of the heavy metals in agricultural soil of Shanghai Baoshan District were analyzed with the methods of combining multivariate statistics and geo-statistical. The results show that the average concentrations of the Cd, Hg, As, Cr, Pb, Cu and Zn in agricultural soil of Baoshan District are 0.195, 0.

[Effect of heat-reinforcing needling combined with rehabilitation training on the motor function of ischemic stroke patients].

Objective: To observe the therapeutic effect of heat-reinforcing needling combined with modem rehabilitation training on the motor function of ischemic stroke patients.

Methods: Fifty case of ischemic stroke patients were randomly divided into rehabilitation (Rehab, n=40) and acupuncture (Acup) + Rehab (n=40) groups. Heat-reinforcing needling was applied to Jianyu (LI 15), Quchi (LI 11), Hegu (LI 14), Zusanli (ST 36), Yanglingquan (GB 34), Yinlingquan (SP 9) and Sanyinjiao (SP 6), once daily for 3 weeks.

A fast predicting neural fuzzy model for on-line estimation of nutrient dynamics in an anoxic/oxic process.

In this paper a software sensor based on a fuzzy neural network approach was proposed for real-time estimation of nutrient concentrations. In order to improve the network performance, fuzzy subtractive clustering was used to identify model architecture, extract and optimize fuzzy rule of the model. A split network structure was applied separately for anaerobic and aerobic conditions was employed with dynamic modeling methods such as autoregressive with exogenous inputs and multi-way principal component analysis (MPCA).

[Regulation effects of various training modes on source-sink relation of cotton].

With the removal of vegetative branches plus the maintenance of early fruiting branches as the control, the effects of various training modes on the source-sink relation of field-grown cotton were examined in 2005 and 2006. The results showed that compared with the control, either the removal of vegetative branches plus the removal of early fruiting branches (I), the maintenance of vegetative branches plus the removal of early fruiting branches (II), or the maintenance of vegetative branches plus the maintenance of early fruiting branches (III) could increase the leaf area per plant significantly, with the average maximum LAI across the two years increased by 10.3%, 28.

A novel method of analyzing proline synonymous codons in E. coli.

Proline is a special imino acid in protein and the isomerization of the prolyl peptide bond has notable biological significance and influences the final structure of protein greatly, so the correlation between proline synonymous codon usage and local amino acid, the correlation between proline synonymous codon usage and the isomerization of the prolyl peptide bond were both investigated in the Escherichia coli genome by using a novel method based on information theory. The results show that in peptide chain, the residue at the first position C-terminal influences the usage of proline synonymous codon greatly and proline synonymous codons contain some factors influencing the isomerization of the prolyl peptide bond.

Cooperativity of the oxidization of cysteines in globular proteins.

Based on the 639 non-homologous proteins with 2910 cysteine-containing segments of well-resolved three-dimensional structures, a novel approach has been proposed to predict the disulfide-bonding state of cysteines in proteins by constructing a two-stage classifier combining a first global linear discriminator based on their amino acid composition and a second local support vector machine classifier. The overall prediction accuracy of this hybrid classifier for the disulfide-bonding state of cysteines in proteins has scored 84.1% and 80.

Prediction of the disulfide-bonding state of cysteines in proteins based on dipeptide composition.

In this paper, a novel approach has been introduced to predict the disulfide-bonding state of cysteines in proteins by means of a linear discriminator based on their dipeptide composition. The prediction is performed with a newly enlarged dataset with 8114 cysteine-containing segments extracted from 1856 non-homologous proteins of well-resolved three-dimensional structures. The oxidation of cysteines exhibits obvious cooperativity: almost all cysteines in disulfide-bond-containing proteins are in the oxidized form.

Related dipeptide and characteristic dipeptide of optimal pH in alpha-amylase.

Alpha-amylase is an enzyme of great significance to industry, but most alpha-amylases are unstable at lower pH. In this paper, we have studied the related dipeptide and characteristic dipeptide of optimal pH in alpha-amylase. On analysis, it gives the explicit results as follows: (1) Ten dipeptides are associated with alpha-amylase's optimal pH.