In silico genome-wide analysis of homeodomain-leucine zipper transcription factors in L.

HD-Zip (Homeodomain-Leucine Zipper) is a family of transcription factors unique to higher plants and plays a vital role in plant growth and development. Increasing research results show that HD-Zip transcription factors are widely involved in many life processes in plants. However, the HD-Zip transcription factor for cannabis, a valuable crop, has not yet been identified.

Effects of Temperature, Moisture, and Ultraviolet Light on Germination, Infection, and Survival of Teliospores and Basidiospores.

Apple rust caused by is a significant disease in China's main apple production areas. We evaluated the effects of temperature, moisture, and ultraviolet (UV) light on the germination, infection, and survival of teliospore horns and basidiospores under artificially controlled environmental conditions. The temperature required for the germination and infection of teliospores and basidiospores of ranged from 5 to 25°C, with an optimum temperature of approximately 17°C.

Genome-Wide Analysis of B3 Transcription Factors in L.

The B3 transcription factor has been identified in , , and , among other species. This family of transcription factors regulates seed growth, development, and stress. Cannabis is a valuable crop with numerous applications; however, no B3 transcription factors have been identified in this plant.

[Transcriptomics of therapeutic effect of Huangqi Liuyi decoction in treating type 2 diabetes].

In this study, Illumina sequencing platform was applied in sequencing rat pancreas, counting expression of target points, analyzing expression differences among blank group, model group and Huangqi Liuyi decoction group and exploring the therapeutic effect and mechanism of Huangqi Liuyi decoction on type 2 diabetes mellitus. According to the result, 24.25% of these genes belonged to the unknown functional class, which was the largest classification unit according to the classification analysis of genes by eggNOG.

[Application of high resolution melting curve to identification of Cimicifugae Rhizoma].

High-resolution-melting analysis (HRM) is a new technology derived from q PCR and is widely used in the study of polymorphism, genotyping, and single nucleotide mutation. Advantages of HRM include cost-effectiveness and time-efficiency over PCR-based genotyping. However, the application of HRM in the authentication of herbal products is still limited with few studies on the classification and identification of herbal products.

[Model Predicts of Astragalus membranceus Transcriptional Gene].

Objective: Astragali Radix, one of the most widely used traditional Chinese medicines, has been extracted a variety of efficiently ingredients. However, the formation mechanism of the secondary metabolites is still unclear and the genome of Astragali Radix has not been sequenced yet. This study aimed at predicting gene model according to transcriptome data, which can be helpful to the use of Astragalus membranceus in the medicine field.

[Study on quality evaluation of sequence and SSR information in transcriptome of Astragalus membranacus].

In this study, 454/Roche GS FLX sequencing technology was used to obtain the data of the Astragalus membranaceus. Four hundred and fifty-four Sequencing System Software was applied to carry out the transcription of the group from scratch. Using MISA tools, 9 893 unigenes were selected for the sequence of the genome of A.

[Identification of cattail pollen (puhuang), pine pollen (songhuafen) and its adulterants by ITS2 sequence].

DNA barcoding method was conducted for the authentication of pollen materials due to difficulty of discriminating pollen materials bearing morphological similarity. In this study, a specific focus was to identify cattail pollen (Puhuang) and pine pollen (Songhuafen) samples from their adulterants which are frequently mixed-together. Regions of the internal transcribed spacer (ITS2) from 60 samples were sequenced, and new primers for cattail pollen were designed according to the sequence information.

[Identification of cimicifugae rhizoma and its adulterants using ITS2 sequence].

In order to identify Cimicifugae Rhizoma from its adulterants and to ensure its safe use, the internal transcribed spacer 2 (ITS2) sequence of Cimicifugae Rhizoma and its adulterants were amplified and bidirectionally sequenced by DNA barcoding technology. Sequence assembly and consensus sequence generation were performed by the CodonCode Aligner V3.7.

[Identification of moutan cortex and its adulterants by ITS2 sequence].

To explore a new method to identify Moutan Cortex to guarantee its safe use, internal transcribed spacer 2 (ITS2) sequence was used to identify Moutan Cortex and its adulterants. DNA was extracted and target fragments were amplified. Sequences were analyzed and assembled by CodonCode Aligner V3.